Archive BacDiveID:10.13145/bacdive5401.20221219.7.1

Strain identifier

BacDive ID: 5401

Type strain:

Species: Acetobacterium wieringae

Strain Designation: C

Strain history: <- M. Braun, strain C

NCBI tax ID(s): 52694 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 924

BacDive-ID: 5401

DSM-Number: 1911

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Acetobacterium wieringae C is an anaerobe, mesophilic bacterium that was isolated from anaerobic sewage digester.

NCBI tax id

NCBI tax id: 52694
Matching level: species

strain history: <- M. Braun, strain C

doi: 10.13145/bacdive5401.20221219.7.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Eubacteriales
family: Eubacteriaceae
genus: Acetobacterium
species: Acetobacterium wieringae
full scientific name: Acetobacterium wieringae Braun and Gottschalk 1983

@ref: 924

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Eubacteriaceae

genus: Acetobacterium

species: Acetobacterium wieringae

full scientific name: Acetobacterium wieringae Braun and Gottschalk 1983

strain designation: C

type strain: yes

Morphology

cell morphology

@ref: 69480
gram stain: positive
confidence: 68.018

Culture and growth conditions

culture medium

@ref: 924
name: ACETOBACTERIUM MEDIUM (DSMZ Medium 135)
growth: yes
link: https://mediadive.dsmz.de/medium/135
composition: Name: ACETOBACTERIUM MEDIUM (DSMZ Medium 135) Composition: NaHCO3 9.79432 g/l D-Fructose 9.79432 g/l Yeast extract 1.95886 g/l NH4Cl 0.979432 g/l Na2S x 9 H2O 0.489716 g/l L-Cysteine HCl x H2O 0.489716 g/l K2HPO4 0.440744 g/l KH2PO4 0.323213 g/l MgSO4 x 7 H2O 0.0587659 g/l Nitrilotriacetic acid 0.029383 g/l NaCl 0.0195886 g/l MnSO4 x H2O 0.00979432 g/l ZnSO4 x 7 H2O 0.00352595 g/l CoSO4 x 7 H2O 0.00352595 g/l FeSO4 x 7 H2O 0.00195886 g/l CaCl2 x 2 H2O 0.00195886 g/l NiCl2 x 6 H2O 0.000587659 g/l Sodium resazurin 0.000489716 g/l AlK(SO4)2 x 12 H2O 0.000391773 g/l CuSO4 x 5 H2O 0.000195886 g/l H3BO3 0.000195886 g/l Na2MoO4 x 2 H2O 0.000195886 g/l Pyridoxine hydrochloride 9.79432e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Folic acid 1.95886e-05 g/l Biotin 1.95886e-05 g/l Na2WO4 x 2 H2O 7.83546e-06 g/l Na2SeO3 x 5 H2O 5.87659e-06 g/l Vitamin B12 9.79432e-07 g/l Distilled water

culture temp

@ref: 924
growth: positive
type: growth
temperature: 30
range: mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
924	anaerobe
69480	anaerobe	99.999

spore formation

@ref: 69480
spore formation: no
confidence: 71.563

halophily

@ref: 69480
halophily level: non-halophilic
confidence: 97.865

Isolation, sampling and environmental information

isolation

@ref: 924
sample type: anaerobic sewage digester
geographic location: Göttingen
country: Germany
origin.country: DEU
continent: Europe

isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)

taxonmaps

@ref: 69479
File name: preview.99_3721.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_30;96_1134;97_1330;98_1608;99_3721&stattab=map
Last taxonomy: Acetobacterium
16S sequence: X96955
Sequence Identity:
Total samples: 1926
soil counts: 102
aquatic counts: 1132
animal counts: 647
plant counts: 45

Safety information

risk assessment

@ref: 924
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 924
description: A.wieringae 16S rRNA gene
accession: X96955
length: 1474
database: ena
NCBI tax ID: 52694

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Acetobacterium wieringae DSM 1911	GCA_001766835	contig	ncbi	52694
66792	Acetobacterium wieringae strain DSM 1911	52694.3	wgs	patric	52694
66792	Acetobacterium wieringae C	2690315663	draft	img	52694

GC content

@ref: 924
GC-content: 43.0

External links

@ref: 924

culture collection no.: DSM 1911, ATCC 43740, JCM 2380

straininfo link

@ref	passport
20218	http://www.straininfo.net/strains/668
20218	http://www.straininfo.net/strains/666
20218	http://www.straininfo.net/strains/669

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Genetics	28007862	Genome Sequence of the Acetogenic Bacterium Acetobacterium wieringae DSM 1911T.	Poehlein A, Bengelsdorf FR, Schiel-Bengelsdorf B, Daniel R, Durre P	Genome Announc	10.1128/genomeA.01430-16	2016
Phylogeny	32082285	Enrichment of Anaerobic Syngas-Converting Communities and Isolation of a Novel Carboxydotrophic Acetobacterium wieringae Strain JM.	Arantes AL, Moreira JPC, Diender M, Parshina SN, Stams AJM, Alves MM, Alves JI, Sousa DZ	Front Microbiol	10.3389/fmicb.2020.00058	2020
Metabolism	33990298	Propionate Production from Carbon Monoxide by Synthetic Cocultures of Acetobacterium wieringae and Propionigenic Bacteria.	Moreira JPC, Diender M, Arantes AL, Boeren S, Stams AJM, Alves MM, Alves JI, Sousa DZ	Appl Environ Microbiol	10.1128/AEM.02839-20	2021	Acetobacterium/drug effects/metabolism, Bacterial Proteins/genetics/metabolism, Carbon Monoxide/metabolism, Coculture Techniques, Deltaproteobacteria/drug effects/metabolism, Fermentation, Propionates/metabolism, Proteome/metabolism, Sodium Acetate/pharmacology	Pathogenicity

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
924	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1911)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-1911
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)