Strain identifier
BacDive ID: 5379
Type strain: 
Species: Sharpea azabuensis
Strain Designation: RL 1
Strain history: <- M.E. Sharpe, RL 1
NCBI tax ID(s): 322505 (species)
version 8.1 (current version)
General
@ref: 8792
BacDive-ID: 5379
DSM-Number: 20406
keywords: genome sequence, Bacteria, anaerobe, mesophilic
description: Sharpea azabuensis RL 1 is an anaerobe, mesophilic bacterium that was isolated from calf rumen.
NCBI tax id
- NCBI tax id: 322505
- Matching level: species
strain history
- @ref: 8792
- history: <- M.E. Sharpe, RL 1
doi: 10.13145/bacdive5379.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Erysipelotrichia
- order: Erysipelotrichales
- family: Coprobacillaceae
- genus: Sharpea
- species: Sharpea azabuensis
- full scientific name: Sharpea azabuensis Morita et al. 2008
@ref: 8792
domain: Bacteria
phylum: Firmicutes
class: Erysipelotrichia
order: Erysipelotrichales
family: Coprobacillaceae
genus: Sharpea
species: Sharpea azabuensis
full scientific name: Sharpea azabuensis Morita et al. 2008
strain designation: RL 1
type strain: no
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 92.914 | |
| 69480 | 99.999 | positive |
Culture and growth conditions
culture medium
- @ref: 8792
- name: MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232)
- growth: yes
- link: https://mediadive.dsmz.de/medium/232
- composition: Name: MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)2 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
culture temp
- @ref: 8792
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 8792 | anaerobe | |
| 69480 | anaerobe | 99.163 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 100 |
murein
- @ref: 8792
- murein short key: A31
- type: A1gamma m-Dpm-direct
Isolation, sampling and environmental information
isolation
- @ref: 8792
- sample type: calf rumen
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Bovinae (Cow, Cattle) |
| #Host Body-Site | #Organ | #Rumen |
Safety information
risk assessment
- @ref: 8792
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Sharpea azabuensis DSM 20406 | GCA_900108995 | contig | ncbi | 322505 |
| 66792 | Sharpea azabuensis strain DSM 20406 | 322505.6 | wgs | patric | 322505 |
| 66792 | Sharpea azabuensis DSM 20406 | 2606217806 | draft | img | 322505 |
GC content
- @ref: 8792
- GC-content: 34.9
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| gram-positive | yes | 94.991 | no |
| anaerobic | yes | 99.287 | yes |
| halophile | no | 73.168 | no |
| spore-forming | no | 80.43 | no |
| glucose-util | yes | 86.602 | no |
| motile | no | 89.672 | no |
| flagellated | no | 93.183 | no |
| aerobic | no | 99.446 | yes |
| thermophile | no | 97.412 | no |
| glucose-ferment | yes | 79.776 | no |
External links
@ref: 8792
culture collection no.: DSM 20406, ATCC 27784, JCM 8229
straininfo link
- @ref: 74869
- straininfo: 39174
literature
- topic: Metabolism
- Pubmed-ID: 31100055
- title: Sharpea azabuensis: a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid.
- authors: Dewanckele L, Vlaeminck B, Fievez V
- journal: Microbiology (Reading)
- DOI: 10.1099/mic.0.000811
- year: 2019
- mesh: Animals, Butyrivibrio/chemistry/genetics/metabolism, Cattle/microbiology, Horses/microbiology, Lactobacillaceae/chemistry/genetics/*metabolism, Linoleic Acid/chemistry/*metabolism, Molecular Structure, Rumen/*microbiology, alpha-Linolenic Acid/chemistry/*metabolism
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 8792 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20406) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20406 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 74869 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID39174.1 | StrainInfo: A central database for resolving microbial strain identifiers |