Strain identifier

BacDive ID: 5372

Type strain: Yes

Species: Erysipelothrix inopinata

Strain Designation: 02-143, MF-EP02

Strain history: CIP <- 2003, DSMZ <- H. Rheims:strain MF-EP02

NCBI tax ID(s): 1121873 (strain), 225084 (species)

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General

@ref: 5969

BacDive-ID: 5372

DSM-Number: 15511

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Erysipelothrix inopinata 02-143 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from vegetable CSB medium.

NCBI tax id

NCBI tax idMatching level
225084species
1121873strain

strain history

@refhistory
5969<- H. Rheims; MF-EP02
120133CIP <- 2003, DSMZ <- H. Rheims:strain MF-EP02

doi: 10.13145/bacdive5372.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Erysipelotrichia
  • order: Erysipelotrichales
  • family: Erysipelotrichaceae
  • genus: Erysipelothrix
  • species: Erysipelothrix inopinata
  • full scientific name: Erysipelothrix inopinata Verbarg et al. 2004

@ref: 5969

domain: Bacteria

phylum: Firmicutes

class: Erysipelotrichia

order: Erysipelotrichales

family: Erysipelotrichaceae

genus: Erysipelothrix

species: Erysipelothrix inopinata

full scientific name: Erysipelothrix inopinata Verbarg et al. 2004 emend. Tegtmeier et al. 2016

strain designation: 02-143, MF-EP02

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30066positive1.5-3.0 µm0.5 µmrod-shapedno
120133positiverod-shapedno

colony morphology

  • @ref: 5969
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

@refproductionname
30066yes
120133noPyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5969COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5969BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
36668MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120133CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5969positivegrowth30mesophilic
30066positivegrowth20-40
30066positiveoptimum25-30mesophilic
36668positivegrowth30mesophilic
120133positivegrowth25-37mesophilic
120133nogrowth5psychrophilic
120133nogrowth10psychrophilic
120133nogrowth41thermophilic

culture pH

  • @ref: 30066
  • ability: positive
  • type: optimum
  • pH: 8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5969microaerophile
120133facultative anaerobe

spore formation

  • @ref: 30066
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
120133NaClpositivegrowth0-4 %
120133NaClnogrowth6 %
120133NaClnogrowth8 %
120133NaClnogrowth10 %

observation

  • @ref: 30066
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3006617057cellobiose+carbon source
3006628757fructose+carbon source
3006617234glucose+carbon source
3006617754glycerol+carbon source
3006651850methyl pyruvate+carbon source
3006617814salicin+carbon source
3006627082trehalose+carbon source
3006616704uridine+carbon source
12013316947citrate-carbon source
1201334853esculin+hydrolysis
12013317632nitrate-reduction
12013316301nitrite-reduction
12013317632nitrate+respiration
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

antibiotic resistance

  • @ref: 120133
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
12013335581indoleno
6838115688acetoinno

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
5969catalase-1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
120133oxidase-
120133beta-galactosidase+3.2.1.23
120133alcohol dehydrogenase-1.1.1.1
120133gelatinase-
120133amylase-
120133DNase-
120133caseinase-3.4.21.50
120133catalase+1.11.1.6
120133tween esterase+
120133lecithinase-
120133lipase-
120133lysine decarboxylase-4.1.1.18
120133ornithine decarboxylase-4.1.1.17
120133protease-
120133tryptophan deaminase-
120133urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120133--++-+----++---+----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5969-++-------+--------++-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120133------------------+----------------------------------------+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5969vegetable CSB mediumNordrhein-WestfalenGermanyDEUEurope
120133Food, VegetableGermanyDEUEurope2002

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Host#Plants
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_39680.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15772;96_18597;97_23004;98_29006;99_39680&stattab=map
  • Last taxonomy: Erysipelothrix inopinata subclade
  • 16S sequence: AJ550617
  • Sequence Identity:
  • Total samples: 1380
  • soil counts: 159
  • aquatic counts: 368
  • animal counts: 734
  • plant counts: 119

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59691Risk group (German classification)
1201331Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5969
  • description: Erysipelothrix inopinata 16S rRNA gene, strain 143-02
  • accession: AJ550617
  • length: 1504
  • database: ena
  • NCBI tax ID: 225084

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Erysipelothrix inopinata DSM 15511GCA_014396165completencbi225084
66792Erysipelothrix inopinata strain DSM 15511225084.5completepatric225084
66792Erysipelothrix inopinata DSM 155112894492305completeimg1121873

GC content

@refGC-contentmethod
3006637.5
596937.5high performance liquid chromatography (HPLC)

External links

@ref: 5969

culture collection no.: DSM 15511, CIP 107935

straininfo link

  • @ref: 74862
  • straininfo: 129387

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14742484Erysipelothrix inopinata sp. nov., isolated in the course of sterile filtration of vegetable peptone broth, and description of Erysipelotrichaceae fam. nov.Verbarg S, Rheims H, Emus S, Fruhling A, Kroppenstedt RM, Stackebrandt E, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.02898-02004DNA, Ribosomal/genetics, Erysipelothrix/*classification/genetics/isolation & purification, Molecular Sequence Data, Phylogeny, Plant Extracts, RNA, Ribosomal, 16S/genetics, Terminology as Topic, Vegetables/*microbiologyGenetics
Phylogeny25534074Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae).Bang BH, Rhee MS, Chang DH, Park DS, Kim BCAntonie Van Leeuwenhoek10.1007/s10482-014-0342-x2014Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cluster Analysis, Coleoptera/microbiology, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Erysipelothrix/*classification/*isolation & purification, Fatty Acids/analysis, Gastrointestinal Tract/microbiology, Glycolipids/analysis, Hydrogen-Ion Concentration, Korea, Larva/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
5969Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15511)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15511
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30066Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2642628776041
36668Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5501
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74862Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID129387.1StrainInfo: A central database for resolving microbial strain identifiers
120133Curators of the CIPCollection of Institut Pasteur (CIP 107935)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107935