Strain identifier

BacDive ID: 5358

Type strain: Yes

Species: Enterococcus quebecensis

Strain Designation: CCRI-16985, EN-66

Strain history: <- A. Maheux, Centre de recherche en infectiologie, Québec, Canada; EN-66 <- P. Cantin, Ministère du Développement durable, de l'Environnement et des Parcs du Québec, Canada

NCBI tax ID(s): 903983 (species)

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General

@ref: 16797

BacDive-ID: 5358

DSM-Number: 23327

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic

description: Enterococcus quebecensis CCRI-16985 is a microaerophile, spore-forming, mesophilic bacterium that was isolated from well water.

NCBI tax id

  • NCBI tax id: 903983
  • Matching level: species

strain history

  • @ref: 16797
  • history: <- A. Maheux, Centre de recherche en infectiologie, Québec, Canada; EN-66 <- P. Cantin, Ministère du Développement durable, de l'Environnement et des Parcs du Québec, Canada

doi: 10.13145/bacdive5358.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus quebecensis
  • full scientific name: Enterococcus quebecensis Sistek et al. 2012

@ref: 16797

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus quebecensis

full scientific name: Enterococcus quebecensis Sistek et al. 2012

strain designation: CCRI-16985, EN-66

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no96.686
69480100positive

colony morphology

  • @ref: 16797
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16797TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
16797COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
16797positivegrowth37mesophilic
61899positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16797microaerophile
61899aerobe

spore formation

@refspore formationconfidence
69481yes100
69480no99.964

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine-hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol+builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan+builds acid from
6838117306maltose+builds acid from
6838128053melibiose+builds acid from
683816731melezitose+builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose+builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
16797catalase-1.11.1.6
16797cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
16797-+----+++++++-+/-+--++++/-+-+++++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16797well waterQuébec, St. MaloCanadaCANNorth America
61899Well waterQuébec,St-MaloCanadaCANNorth America2005-10-19

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Engineered#Built environment#Water reservoir (Aquarium/pool)

Safety information

risk assessment

  • @ref: 16797
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16797
  • description: Enterococcus quebecensis strain CCRI-16985 16S ribosomal RNA gene, partial sequence
  • accession: GU457262
  • length: 1290
  • database: ena
  • NCBI tax ID: 903983

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus quebecensis LMG 26306GCA_001730365contigncbi903983
66792Enterococcus quebecensis DSM 23327GCA_001886145scaffoldncbi903983
66792Enterococcus quebecensis strain DSM 23327903983.4wgspatric903983
66792Enterococcus quebecensis strain LMG 26306903983.3wgspatric903983

GC content

  • @ref: 16797
  • GC-content: 43
  • method: fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno97.039no
gram-positiveyes92.464no
anaerobicno95.056yes
aerobicno95.826no
halophileyes85.157no
spore-formingno83.923no
glucose-utilyes88.252no
thermophileno99.52yes
motileno95.631no
glucose-fermentyes87.623no

External links

@ref: 16797

culture collection no.: DSM 23327, CCUG 59306, LMG 26306

straininfo link

  • @ref: 74848
  • straininfo: 376040

literature

  • topic: Phylogeny
  • Pubmed-ID: 21788227
  • title: Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water.
  • authors: Sistek V, Maheux AF, Boissinot M, Bernard KA, Cantin P, Cleenwerck I, De Vos P, Bergeron MG
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.029033-0
  • year: 2011
  • mesh: Bacterial Proteins/genetics, DNA, Bacterial/genetics, Enterococcus/*classification/genetics/*isolation & purification, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16797Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23327)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23327
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
61899Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 59306)https://www.ccug.se/strain?id=59306
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74848Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID376040.1StrainInfo: A central database for resolving microbial strain identifiers