Strain identifier

BacDive ID: 5346

Type strain: Yes

Species: Enterococcus aquimarinus

Strain history: CIP <- 2005, CCM <- 2005, LMG <- L. Devriese, Ghent Univ., Ghent, Belgium <- BioMérieux <- L. Volterra

NCBI tax ID(s): 328396 (species)

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General

@ref: 7131

BacDive-ID: 5346

DSM-Number: 17690

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Enterococcus aquimarinus DSM 17690 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from sea water.

NCBI tax id

  • NCBI tax id: 328396
  • Matching level: species

strain history

@refhistory
7131<- CCM
116457CIP <- 2005, CCM <- 2005, LMG <- L. Devriese, Ghent Univ., Ghent, Belgium <- BioMérieux <- L. Volterra

doi: 10.13145/bacdive5346.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus aquimarinus
  • full scientific name: Enterococcus aquimarinus Švec et al. 2005

@ref: 7131

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus aquimarinus

full scientific name: Enterococcus aquimarinus Švec et al. 2005

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31516positiverod-shapedno
69480positive100

colony morphology

  • @ref: 7131
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

pigmentation

  • @ref: 31516
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7131COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7131TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38378MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116457CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
7131positivegrowth37mesophilic
31516positivegrowth25-42
31516positiveoptimum33.5mesophilic
38378positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 7131
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.903

halophily

@refsaltgrowthtested relationconcentration
31516NaClpositivegrowth6.5 %
31516NaClpositiveoptimum6.5 %

observation

  • @ref: 31516
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3151622599arabinose+carbon source
3151617057cellobiose+carbon source
3151628757fructose+carbon source
3151628260galactose+carbon source
3151617234glucose+carbon source
3151617716lactose+carbon source
3151617306maltose+carbon source
3151637684mannose+carbon source
3151628053melibiose+carbon source
31516506227N-acetylglucosamine+carbon source
3151616634raffinose+carbon source
3151617814salicin+carbon source
3151617992sucrose+carbon source
3151618222xylose+carbon source
315164853esculin+hydrolysis
6837028087glycogen-builds acid from
6837028017starch+builds acid from
6837016634raffinose+builds acid from
6837015443inulin+builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol-builds acid from
6837016899D-mannitol-builds acid from
6837030849L-arabinose+builds acid from
6837016988D-ribose-builds acid from
6837029016arginine-hydrolysis
683704853esculin+hydrolysis
68370606565hippurate-hydrolysis
6838129016arginine-hydrolysis
6838130911sorbitol-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
683816731melezitose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
6837015688acetoin-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381beta-glucuronidase-3.2.1.31
68381arginine dihydrolase-3.5.3.6
68370arginine dihydrolase-3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase-3.1.3.1
68370beta-galactosidase+3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370alpha-galactosidase+3.2.1.22
68370pyrrolidonyl arylamidase+3.4.19.3
68370beta-glucosidase+3.2.1.21

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYG
7131--+++-+-+--+--+++++-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
7131--+-+----+++++----++-+---++-----
7131----+----++/-++-----++-+---++-----
7131-++-+----+++++----++-+---++-++--
7131--+-+----+/-+++-----++-----++-----
7131-++--+++-++--+----++++---+--++/-+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
7131sea waterItalyITAEurope
116457Environment, Sea waterItalyITAEuropeRome

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_5572.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_1097;97_3304;98_4158;99_5572&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: AJ877015
  • Sequence Identity:
  • Total samples: 3709
  • soil counts: 141
  • aquatic counts: 2709
  • animal counts: 799
  • plant counts: 60

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71311Risk group (German classification)
1164572Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus aquimarinus strain LMG 16607 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU089781278ena328396
7131Enterococcus aquamarinus 16S rRNA gene, type strain LMG 16607TAJ8770151510ena328396

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus aquimarinus DSM 17690GCA_001885765contigncbi328396
66792Enterococcus aquimarinus strain DSM 17690328396.3wgspatric328396

GC content

  • @ref: 7131
  • GC-content: 38.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes58no
gram-positiveyes94.155yes
anaerobicno97.78yes
halophileyes90.914yes
spore-formingno88.901no
glucose-utilyes89.863no
motileno95.186no
flagellatedno97.594yes
aerobicno94.889yes
thermophileno99.389no
glucose-fermentyes84.39no

External links

@ref: 7131

culture collection no.: DSM 17690, CCM 7283, CCUG 51308, CIP 108926, LMG 16607, API 8407116

straininfo link

  • @ref: 74835
  • straininfo: 8419

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16166729Enterococcus aquimarinus sp. nov., isolated from sea water.Svec P, Vancanneyt M, Devriese LA, Naser SM, Snauwaert C, Lefebvre K, Hoste B, Swings JInt J Syst Evol Microbiol10.1099/ijs.0.63722-02005Bacterial Typing Techniques, DNA Fingerprinting/methods, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Enterococcus/chemistry/*classification/genetics/isolation & purification, Genes, rRNA, Molecular Sequence Data, Phenotype, Phenylalanine-tRNA Ligase/genetics, Phylogeny, RNA, Ribosomal, 16S, Seawater/*microbiology, Sequence Analysis, DNAGenetics
Phylogeny23416573Enterococcus alcedinis sp. nov., isolated from common kingfisher (Alcedo atthis).Frolkova P, Svec P, Sedlacek I, Maslanova I, Cernohlavkova J, Ghosh A, Zurek L, Radimersky T, Literak IInt J Syst Evol Microbiol10.1099/ijs.0.049833-02013Animals, Bacterial Typing Techniques, Base Composition, Birds/*microbiology, Czech Republic, DNA, Bacterial/genetics, DNA-Directed RNA Polymerases/genetics, Enterococcus/*classification/genetics/isolation & purification, Genes, Bacterial, Molecular Sequence Data, Peptide Mapping, Phenylalanine-tRNA Ligase/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Superoxide Dismutase/geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7131Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17690)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17690
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31516Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127811
38378Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6610
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74835Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8419.1StrainInfo: A central database for resolving microbial strain identifiers
116457Curators of the CIPCollection of Institut Pasteur (CIP 108926)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108926