Strain identifier

BacDive ID: 5342

Type strain: Yes

Species: Enterococcus italicus

Strain Designation: TP 1.5

Strain history: CIP <- 2004, M.G. Fortina, Milan Univ., Milan, Italy: strain TP 1.5 <- G. Ricci

NCBI tax ID(s): 888064 (strain), 246144 (species)

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General

@ref: 6206

BacDive-ID: 5342

DSM-Number: 15952

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Enterococcus italicus TP 1.5 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from toma cheese.

NCBI tax id

NCBI tax idMatching level
246144species
888064strain

strain history

@refhistory
6206<- M. G. Fortina; TP 1.5 <- G. Ricci
120291CIP <- 2004, M.G. Fortina, Milan Univ., Milan, Italy: strain TP 1.5 <- G. Ricci

doi: 10.13145/bacdive5342.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus italicus
  • full scientific name: Enterococcus italicus Fortina et al. 2004

  • synonyms

    • @ref: 20215
    • synonym: Enterococcus saccharominimus

@ref: 6206

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus italicus

full scientific name: Enterococcus italicus Fortina et al. 2004

strain designation: TP 1.5

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31275positivecoccus-shapedno
69480positive100
69480no91.529
120291positivecoccus-shapedno

colony morphology

@reftype of hemolysisincubation period
6206gamma1-2 days
120291

pigmentation

  • @ref: 31275
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6206COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6206TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37442MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
120291CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120291CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
6206positivegrowth37mesophilic
31275positivegrowth10-45
31275positiveoptimum37mesophilic
37442positivegrowth37mesophilic
58866positivegrowth37mesophilic
120291positivegrowth25-41
120291nogrowth10psychrophilic
120291nogrowth45thermophilic

culture pH

  • @ref: 31275
  • ability: positive
  • type: optimum
  • pH: 9.6
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6206microaerophile
31275facultative anaerobe
58866microaerophile
120291facultative anaerobe

spore formation

@refspore formationconfidence
31275no
69480no99.982

halophily

@refsaltgrowthtested relationconcentration
31275NaClpositivegrowth05-06 %
31275NaClpositiveoptimum5 %
120291NaClnogrowth6.5 %

observation

  • @ref: 31275
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3127517057cellobiose+carbon source
3127528757fructose+carbon source
3127528260galactose+carbon source
3127517234glucose+carbon source
3127517716lactose+carbon source
3127517306maltose+carbon source
3127537684mannose+carbon source
3127537657methyl D-glucoside+carbon source
31275506227N-acetylglucosamine+carbon source
3127517814salicin+carbon source
3127517992sucrose+carbon source
3127527082trehalose+carbon source
312754853esculin+hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
1202914853esculin+hydrolysis
120291606565hippurate+hydrolysis
12029117632nitrate-reduction
12029116301nitrite-reduction
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol+builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite tests

  • @ref: 120291
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
6206catalase-1.11.1.6
6206cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
120291oxidase-
120291beta-galactosidase-3.2.1.23
120291alcohol dehydrogenase-1.1.1.1
120291gelatinase-
120291amylase-
120291catalase-1.11.1.6
120291gamma-glutamyltransferase+2.3.2.2
120291lysine decarboxylase-4.1.1.18
120291ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120291--++-+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120291+/---------++++----+---+--+/-+/--++-++---+--+---------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
6206-------++/-++-+--++/-+-+-+---+------
6206-------+-+++/-+--++--+-----+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120291+++++--+---++--------------------+-------------------------+--------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6206toma cheesePiedmontItalyITAEurope
58866Cheese,Italian Toma,from bovine milkPiedmontItalyITAEurope
120291Food, Cheese, Toma PiemontesePiemontItalyITAEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_4369.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_1097;97_1285;98_3282;99_4369&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: AJ582753
  • Sequence Identity:
  • Total samples: 16591
  • soil counts: 493
  • aquatic counts: 2257
  • animal counts: 12994
  • plant counts: 847

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
62061Risk group (German classification)
1202912Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6206
  • description: Enterococcus sp. TP1.5 partial 16S rRNA gene, strain TP1.5
  • accession: AJ582753
  • length: 1396
  • database: ena
  • NCBI tax ID: 888064

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus italicus DSM 15952GCA_001885995contigncbi888064
66792Enterococcus italicus DSM 15952GCA_000185365scaffoldncbi888064
66792Enterococcus italicus DSM 15952888064.3wgspatric888064
66792Enterococcus italicus DSM 15952888064.11wgspatric888064
66792Enterococcus italicus DSM 15952649989943draftimg888064

GC content

@refGC-content
620639.9
3127540.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno65no
motileno96.012yes
flagellatedno96.939no
gram-positiveyes96.053yes
anaerobicno95.095yes
aerobicno98.49yes
halophileyes80.762no
spore-formingno92.037yes
glucose-utilyes89.339yes
glucose-fermentyes90.593no
thermophileno99.476yes

External links

@ref: 6206

culture collection no.: DSM 15952, CCUG 50447, LMG 22039, CIP 108583

straininfo link

  • @ref: 74831
  • straininfo: 129721

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15388734Molecular analysis of artisanal Italian cheeses reveals Enterococcus italicus sp. nov.Fortina MG, Ricci G, Mora D, Manachini PLInt J Syst Evol Microbiol10.1099/ijs.0.63190-02004Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Carbohydrate Metabolism, Cheese/*microbiology, DNA Fingerprinting, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, DNA, Ribosomal Spacer/analysis, Enterococcus/*classification/genetics/*isolation & purification/physiology, Food Microbiology, Genes, rRNA, Italy, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/biosynthesis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic/pharmacology, Sequence Analysis, DNA, TemperatureEnzymology
Phylogeny17397485Rapid identification of Enterococcus italicus by PCR with primers targeted to 16S rRNA gene.Fortina MG, Ricci G, Borgo F, Manachini PLLett Appl Microbiol10.1111/j.1472-765X.2006.02082.x2007Cheese/*microbiology, DNA Primers, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Enterococcus/classification/genetics/*isolation & purification, Polymerase Chain Reaction/*methods, RNA, Ribosomal, 16S/genetics, Sensitivity and Specificity, Species SpecificityEnzymology
Genetics23462976Genomic analysis reveals the biotechnological ability of Enterococcus italicus to produce glutathione.Borgo F, Carpen A, Ferrario C, Iametti S, Fortina MGJ Ind Microbiol Biotechnol10.1007/s10295-013-1239-92013Amino Acid Sequence, Bacterial Proteins/genetics/metabolism, *Bioreactors, Enterococcus/enzymology/*genetics/*metabolism, Fermentation, Genome, Bacterial/genetics, *Genomics, Glutathione/*biosynthesisMetabolism
Phylogeny26346480Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk.Kadri Z, Spitaels F, Cnockaert M, Praet J, El Farricha O, Swings J, Vandamme PAntonie Van Leeuwenhoek10.1007/s10482-015-0579-z2015Animals, Base Composition, Camelus, DNA, Bacterial, Enterococcus/chemistry/*classification/genetics/isolation & purification/*metabolism, *Food Microbiology, Lactic Acid/*biosynthesis, Milk/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBiotechnology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
6206Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15952)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15952
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31275Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2759528776041
37442Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6227
58866Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50447)https://www.ccug.se/strain?id=50447
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74831Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID129721.1StrainInfo: A central database for resolving microbial strain identifiers
120291Curators of the CIPCollection of Institut Pasteur (CIP 108583)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108583