Strain identifier

BacDive ID: 5341

Type strain: Yes

Species: Enterococcus haemoperoxidus

Strain Designation: Svec 440, 440

Strain history: CIP <- 2001, CCM <- B. Otipka: strain 440

NCBI tax ID(s): 155618 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6184

BacDive-ID: 5341

DSM-Number: 15920

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic, Gram-positive

description: Enterococcus haemoperoxidus Svec 440 is a microaerophile, spore-forming, mesophilic bacterium that was isolated from service water.

NCBI tax id

  • NCBI tax id: 155618
  • Matching level: species

strain history

@refhistory
6184<- D. Janssens, LMG <- M. Vancanneyt <- P. Svec; Svec 440
119783CIP <- 2001, CCM <- B. Otipka: strain 440

doi: 10.13145/bacdive5341.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus haemoperoxidus
  • full scientific name: Enterococcus haemoperoxidus Švec et al. 2001

@ref: 6184

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus haemoperoxidus

full scientific name: Enterococcus haemoperoxidus Švec et al. 2001

strain designation: Svec 440, 440

type strain: yes

Morphology

cell morphology

@refgram stainconfidencemotilitycell shape
69480positive100
6948093.815no
119783positivenococcus-shaped

colony morphology

@reftype of hemolysis
6184gamma
119783

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6184COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6184TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
36096MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119783CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6184positivegrowth37mesophilic
36096positivegrowth37mesophilic
56979positivegrowth30-37mesophilic
119783positivegrowth10-37
119783nogrowth41thermophilic
119783nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6184microaerophile
56979aerobe
119783facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480no99.969

halophily

  • @ref: 119783
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
119783esculin+hydrolysis4853
119783hippurate+hydrolysis606565
119783nitrate-reduction17632
119783nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate+hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose+builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11978315688acetoin+

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
119783oxidase-
119783beta-galactosidase-3.2.1.23
119783alcohol dehydrogenase-1.1.1.1
119783gelatinase-
119783amylase+
119783catalase-1.11.1.6
119783gamma-glutamyltransferase+2.3.2.2
119783lysine decarboxylase-4.1.1.18
119783ornithine decarboxylase-4.1.1.17

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119783+---+----++++--------++++++++-++-++/-+--+---------+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
6184-+--+-+--++++---+--++++--+-++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119783---------+--------+-------------------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6184service waterPaskovCzech RepublicCZEEurope
56979Water,service (non-potable)PaskovCzech RepublicCZEEurope
119783Environment, Non-drinkable WaterPaskovCzech RepublicCZEEurope1996

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
61841Risk group (German classification)
1197832Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus haemoperoxidus strain LMG 19487 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU089785287ena155618
6184Enterococcus haemoperoxidus 16S ribosomal RNA gene, partial sequenceAF2868321512ena155618

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus haemoperoxidus DSM 15920GCA_001885985scaffoldncbi155618
66792Enterococcus haemoperoxidus strain DSM 15920155618.3wgspatric155618

GC content

  • @ref: 6184
  • GC-content: 35.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno97.688no
gram-positiveyes93.744no
anaerobicno94.981yes
aerobicno92.27no
halophileyes83.586no
spore-formingno85.139no
glucose-utilyes88.139no
thermophileno99.689yes
motileno94.661no
glucose-fermentyes84.689no

External links

@ref: 6184

culture collection no.: CCUG 45916, DSM 15920, CCM 4851, LMG 19487, CIP 107129

straininfo link

  • @ref: 74830
  • straininfo: 13276

literature

  • topic: Phylogeny
  • Pubmed-ID: 11491359
  • title: Enterococcus haemoperoxidus sp. nov. and Enterococcus moraviensis sp. nov., isolated from water.
  • authors: Svec P, Devriese LA, Sedlacek I, Baele M, Vancanneyt M, Haesebrouck F, Swings J, Doskar J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-4-1567
  • year: 2001
  • mesh: Base Composition, Base Sequence, Czech Republic, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Enterococcus/*classification/genetics/*isolation & purification, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Species Specificity, Terminology as Topic, *Water Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6184Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15920)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15920
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36096Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4531
56979Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45916)https://www.ccug.se/strain?id=45916
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74830Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13276.1StrainInfo: A central database for resolving microbial strain identifiers
119783Curators of the CIPCollection of Institut Pasteur (CIP 107129)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107129