Strain identifier

BacDive ID: 5340

Type strain: Yes

Species: Enterococcus moraviensis

Strain Designation: Svec 330, 330

Strain history: CIP <- 2001, CCM <- B. Otipka: strain 330

NCBI tax ID(s): 155617 (species)

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General

@ref: 6183

BacDive-ID: 5340

DSM-Number: 15919

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic

description: Enterococcus moraviensis Svec 330 is a microaerophile, spore-forming, mesophilic bacterium that was isolated from spring Hajek.

NCBI tax id

  • NCBI tax id: 155617
  • Matching level: species

strain history

@refhistory
6183<- LMG <- M. Vancanneyt <- P. Svec; Svec 330
121138CIP <- 2001, CCM <- B. Otipka: strain 330

doi: 10.13145/bacdive5340.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus moraviensis
  • full scientific name: Enterococcus moraviensis Švec et al. 2001

@ref: 6183

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus moraviensis

full scientific name: Enterococcus moraviensis Švec et al. 2001

strain designation: Svec 330, 330

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.834
69480100positive
121138nopositiveovoid-shaped

colony morphology

  • @ref: 121138
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6183COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6183TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33625MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121138CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6183positivegrowth37mesophilic
33625positivegrowth37mesophilic
56976positivegrowth30-37mesophilic
121138positivegrowth10-37
121138nogrowth41thermophilic
121138nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6183microaerophile
56976aerobe
121138facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480no99.985

halophily

  • @ref: 121138
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
121138esculin+hydrolysis4853
121138hippurate+hydrolysis606565
121138nitrate-reduction17632
121138nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate+hydrolysis606565
68381glycogen-builds acid from28087
68381maltose+builds acid from17306
68381melezitose+builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12113815688acetoin+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
121138oxidase-
121138beta-galactosidase+3.2.1.23
121138alcohol dehydrogenase-1.1.1.1
121138gelatinase-
121138amylase-
121138catalase-1.11.1.6
121138gamma-glutamyltransferase-2.3.2.2
121138lysine decarboxylase-4.1.1.18
121138ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121138--++-+-+-+++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121138+--+/-+----++++---+/-+--++++++++--++-+-+--++/--+------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
6183-+--++/-+++/-++++---+-+++++-+/-++/-++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121138++-++--+--+----+-++------+-------+---------+---------------+--------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6183spring HajekPaskovCzech RepublicCZEEurope
56976Water,Spring HájekFrydek-MístekCzech RepublicCZEEurope
121138Environment, Spring HajekCzech RepublicCZEEurope1996

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Spring

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
61831Risk group (German classification)
1211382Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus moraviensis strain LMG 19486 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU089788276ena155617
6183Enterococcus moraviensis 16S ribosomal RNA gene, partial sequenceAF2868311509ena155617

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus moraviensis DSM 15919GCA_001886065contigncbi155617
66792Enterococcus moraviensis strain DSM 15919155617.3wgspatric155617

GC content

  • @ref: 6183
  • GC-content: 36.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.995no
gram-positiveyes94.146no
anaerobicno95.177no
aerobicno93.321no
halophileyes76.006no
spore-formingno84.245no
thermophileno99.183no
glucose-utilyes87.317no
flagellatedno97.786no
glucose-fermentyes79.284no

External links

@ref: 6183

culture collection no.: DSM 15919, CCM 4856, LMG 19486, CCUG 45913, CIP 107130

straininfo link

  • @ref: 74829
  • straininfo: 13275

literature

  • topic: Phylogeny
  • Pubmed-ID: 11491359
  • title: Enterococcus haemoperoxidus sp. nov. and Enterococcus moraviensis sp. nov., isolated from water.
  • authors: Svec P, Devriese LA, Sedlacek I, Baele M, Vancanneyt M, Haesebrouck F, Swings J, Doskar J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-4-1567
  • year: 2001
  • mesh: Base Composition, Base Sequence, Czech Republic, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Enterococcus/*classification/genetics/*isolation & purification, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Species Specificity, Terminology as Topic, *Water Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6183Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15919)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15919
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33625Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4532
56976Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45913)https://www.ccug.se/strain?id=45913
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74829Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13275.1StrainInfo: A central database for resolving microbial strain identifiers
121138Curators of the CIPCollection of Institut Pasteur (CIP 107130)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107130