Strain identifier
BacDive ID: 534
Type strain:
Species: Aureimonas frigidaquae
Strain Designation: CW5
Strain history: <- CN Seong, Sunchon Natl. Univ.
NCBI tax ID(s): 424757 (species)
General
@ref: 16080
BacDive-ID: 534
DSM-Number: 21987
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming, pigmented
description: Aureimonas frigidaquae CW5 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and has a yellow pigmentation.
NCBI tax id
- NCBI tax id: 424757
- Matching level: species
strain history
@ref | history |
---|---|
16080 | <- JCM/RIKEN <- C. N. Seong, Sunchon Natl. Univ., Korea; CW5 |
67770 | C. N. Seong CW5. |
67771 | <- CN Seong, Sunchon Natl. Univ. |
doi: 10.13145/bacdive534.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Aurantimonadaceae
- genus: Aureimonas
- species: Aureimonas frigidaquae
- full scientific name: Aureimonas frigidaquae (Kim et al. 2008) Rathsack et al. 2011
synonyms
- @ref: 20215
- synonym: Aurantimonas frigidaquae
@ref: 16080
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Aurantimonadaceae
genus: Aureimonas
species: Aureimonas frigidaquae
full scientific name: Aureimonas frigidaquae (Kim et al. 2008) Rathsack et al. 2011
strain designation: CW5
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
23136 | negative | 0.8-1.2 µm | 0.6-0.8 µm | rod-shaped | yes | |
67771 | negative | |||||
69480 | negative | 99.806 |
colony morphology
- @ref: 23136
- colony size: 3.0 mm
- colony shape: circular
- incubation period: 5 days
- medium used: TSA
pigmentation
- @ref: 23136
- production: yes
- color: yellow
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
16080 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
16080 | TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) | yes | https://mediadive.dsmz.de/medium/545 | Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water |
16080 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
23136 | PCA, TSA, NA, R2A, MA and MacConkey agar | yes | ||
23136 | Trypticase Soy Agar (TSA) | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16080 | positive | growth | 28 | mesophilic |
23136 | positive | maximum | 37 | mesophilic |
23136 | positive | minimum | 15 | psychrophilic |
23136 | positive | optimum | 25 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
67771 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
23136 | positive | minimum | 5.0 | |
23136 | positive | maximum | 11.0 | alkaliphile |
23136 | positive | optimum | 7.0 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23136 | facultative anaerobe |
67771 | aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.986 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
23136 | NaCl | positive | maximum | <7 %(w/v) |
23136 | NaCl | positive | optimum | 0 %(w/v) |
observation
- @ref: 67770
- observation: quinones: Q-10
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23136 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
23136 | 17426 | 5-dehydro-D-gluconate | - | builds acid from |
23136 | 16443 | D-tagatose | - | builds acid from |
23136 | 16813 | galactitol | - | builds acid from |
23136 | 24265 | gluconate | - | builds acid from |
23136 | 17754 | glycerol | - | builds acid from |
23136 | 28087 | glycogen | - | builds acid from |
23136 | 15443 | inulin | - | builds acid from |
23136 | 17716 | lactose | - | builds acid from |
23136 | 17306 | maltose | - | builds acid from |
23136 | 6731 | melezitose | - | builds acid from |
23136 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
23136 | 320055 | methyl beta-D-glucopyranoside | - | builds acid from |
23136 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
23136 | 28017 | starch | - | builds acid from |
23357 | 17521 | (-)-quinic acid | - | carbon source |
23357 | 64552 | 2-hydroxybutyrate | - | carbon source |
23357 | 16763 | 2-oxobutanoate | - | carbon source |
23357 | 28644 | 2-oxopentanoate | - | carbon source |
23357 | 37054 | 3-hydroxybutyrate | - | carbon source |
23357 | 16865 | gamma-aminobutyric acid | - | carbon source |
23357 | 16724 | 4-hydroxybutyrate | - | carbon source |
23357 | 30089 | acetate | - | carbon source |
23357 | 15963 | ribitol | - | carbon source |
23357 | 40585 | alpha-cyclodextrin | - | carbon source |
23357 | 36219 | alpha-lactose | - | carbon source |
23357 | 73706 | bromosuccinate | - | carbon source |
23357 | 17057 | cellobiose | - | carbon source |
23357 | 15570 | D-alanine | - | carbon source |
23357 | 18333 | D-arabitol | - | carbon source |
23357 | 15824 | D-fructose | - | carbon source |
23357 | 15895 | D-galactonic acid lactone | - | carbon source |
23357 | 12936 | D-galactose | - | carbon source |
23357 | 30612 | D-glucarate | - | carbon source |
23357 | 8391 | D-gluconate | - | carbon source |
23357 | 17784 | D-glucosaminic acid | - | carbon source |
23357 | 16899 | D-mannitol | - | carbon source |
23357 | 16024 | D-mannose | - | carbon source |
23357 | 17924 | D-sorbitol | - | carbon source |
23357 | 17126 | DL-carnitine | - | carbon source |
23357 | 17113 | erythritol | - | carbon source |
23357 | 28066 | gentiobiose | - | carbon source |
23357 | 32323 | glucuronamide | - | carbon source |
23357 | 17754 | glycerol | - | carbon source |
23357 | 15978 | glycerol 3-phosphate | - | carbon source |
23357 | 28087 | glycogen | - | carbon source |
23357 | 73804 | glycyl L-aspartic acid | - | carbon source |
23357 | 24741 | hydroxyproline | - | carbon source |
23357 | 17268 | myo-inositol | - | carbon source |
23357 | 17240 | itaconate | - | carbon source |
23357 | 21217 | L-alaninamide | - | carbon source |
23357 | 16977 | L-alanine | - | carbon source |
23357 | 73786 | L-alanylglycine | - | carbon source |
23357 | 30849 | L-arabinose | - | carbon source |
23357 | 17196 | L-asparagine | - | carbon source |
23357 | 29991 | L-aspartate | - | carbon source |
23357 | 18287 | L-fucose | - | carbon source |
23357 | 29985 | L-glutamate | - | carbon source |
23357 | 15971 | L-histidine | - | carbon source |
23357 | 15603 | L-leucine | - | carbon source |
23357 | 15729 | L-ornithine | - | carbon source |
23357 | 17203 | L-proline | - | carbon source |
23357 | 18183 | L-pyroglutamic acid | - | carbon source |
23357 | 62345 | L-rhamnose | - | carbon source |
23357 | 17115 | L-serine | - | carbon source |
23357 | 16857 | L-threonine | - | carbon source |
23357 | 24996 | lactate | - | carbon source |
23357 | 17306 | maltose | - | carbon source |
23357 | 28053 | melibiose | - | carbon source |
23357 | 320061 | methyl alpha-D-glucopyranoside | - | carbon source |
23357 | 75146 | monomethyl succinate | - | carbon source |
23357 | 28037 | N-acetylgalactosamine | - | carbon source |
23357 | 73784 | glycyl-l-glutamate | - | carbon source |
23357 | 17272 | propionate | - | carbon source |
23357 | 33951 | psicose | - | carbon source |
23357 | 17148 | putrescine | - | carbon source |
23357 | 15361 | pyruvate | - | carbon source |
23357 | 16634 | raffinose | - | carbon source |
23357 | 143136 | succinamate | - | carbon source |
23357 | 30031 | succinate | - | carbon source |
23357 | 17992 | sucrose | - | carbon source |
23357 | 17748 | thymidine | - | carbon source |
23357 | 32528 | turanose | - | carbon source |
23357 | 53423 | tween 40 | - | carbon source |
23357 | 16704 | uridine | - | carbon source |
23357 | 27248 | urocanic acid | - | carbon source |
23357 | 17151 | xylitol | - | carbon source |
23136 | 30849 | L-arabinose | - | carbon source |
23136 | 65328 | L-xylose | - | carbon source |
23136 | 17306 | maltose | - | carbon source |
23136 | 26546 | rhamnose | - | carbon source |
23136 | 17234 | glucose | - | fermentation |
23136 | casein | - | hydrolysis | |
23136 | 16991 | dna | - | hydrolysis |
23136 | 5291 | gelatin | - | hydrolysis |
23136 | 28017 | starch | - | hydrolysis |
23136 | 53426 | tween 80 | - | hydrolysis |
23136 | 18186 | tyrosine | - | hydrolysis |
23136 | 16136 | hydrogen sulfide | - | other |
23136 | 15963 | ribitol | + | builds acid from |
23136 | 27613 | amygdalin | + | builds acid from |
23136 | 18305 | arbutin | + | builds acid from |
23136 | 17057 | cellobiose | + | builds acid from |
23136 | 18333 | D-arabitol | + | builds acid from |
23136 | 28847 | D-fucose | + | builds acid from |
23136 | 4853 | esculin | + | builds acid from |
23136 | 28757 | fructose | + | builds acid from |
23136 | 17234 | glucose | + | builds acid from |
23136 | 17268 | myo-inositol | + | builds acid from |
23136 | 18403 | L-arabitol | + | builds acid from |
23136 | 29864 | mannitol | + | builds acid from |
23136 | 506227 | N-acetylglucosamine | + | builds acid from |
23136 | 16634 | raffinose | + | builds acid from |
23136 | 17814 | salicin | + | builds acid from |
23136 | 17992 | sucrose | + | builds acid from |
23357 | 23652 | dextrin | + | carbon source |
23357 | 15740 | formate | + | carbon source |
23136 | 17113 | erythritol | + | carbon source |
23136 | 18287 | L-fucose | + | carbon source |
23136 | 29864 | mannitol | + | carbon source |
23136 | 506227 | N-acetylglucosamine | + | carbon source |
23136 | 4853 | esculin | + | hydrolysis |
23136 | 28066 | gentiobiose | +/- | carbon source |
23136 | 24265 | gluconate | +/- | carbon source |
23136 | 33942 | ribose | +/- | carbon source |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | is sensitive | sensitivity conc. |
---|---|---|---|---|---|---|---|
23136 | 17334 | penicillin | yes | yes | 10 Unit | ||
23136 | 28669 | bacitracin | yes | yes | 10 µg (disc) | ||
23136 | 28001 | vancomycin | yes | yes | 30 µg (disc) | ||
23136 | 8309 | polymyxin b | yes | yes | 300 Unit | ||
23136 | 28971 | ampicillin | yes | yes | 10 µg (disc) | ||
23136 | 17833 | gentamicin | yes | yes | 10 µg (disc) | ||
23136 | 17076 | streptomycin | yes | yes | 10 µg (disc) | ||
23136 | 48923 | erythromycin | yes | yes | 15 µg (disc) | ||
23136 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) | ||
23136 | 6104 | kanamycin | yes | yes | 30 µg (disc) | ||
23136 | 100147 | nalidixic acid | yes | yes | 30 µg (disc) | ||
23136 | 27902 | tetracycline | yes | yes | 30 µg (disc) |
metabolite production
- @ref: 23136
- Chebi-ID: 15688
- metabolite: acetoin
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
23136 | 15688 | acetoin | - | |
23136 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23136 | acid phosphatase | + | 3.1.3.2 |
23136 | alpha-chymotrypsin | - | 3.4.21.1 |
23136 | alpha-fucosidase | - | 3.2.1.51 |
23136 | alpha-glucosidase | - | 3.2.1.20 |
23136 | alpha-mannosidase | - | 3.2.1.24 |
23136 | beta-glucuronidase | - | 3.2.1.31 |
23136 | catalase | + | 1.11.1.6 |
23136 | cystine arylamidase | - | 3.4.11.3 |
23136 | cytochrome oxidase | + | 1.9.3.1 |
23136 | esterase (C 4) | + | |
23136 | esterase lipase (C 8) | + | |
23136 | leucine arylamidase | + | 3.4.11.1 |
23136 | lipase (C 14) | - | |
23136 | lysine decarboxylase | - | 4.1.1.18 |
23136 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
23136 | ornithine decarboxylase | - | 4.1.1.17 |
23136 | valine arylamidase | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
16080 | water-cooling system | Gwangyang | Republic of Korea | KOR | Asia | |
23136 | cooled water sample from an oxygen-producing plant | 2005-02 | ||||
67770 | Water-cooling system in Gwangyang | Republic of Korea | KOR | Asia | ||
67771 | From cooling water | Gwangyang | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Industrial | #Plant (Factory) |
#Environmental | #Aquatic | #Freshwater |
#Condition | #Psychrophilic (<10°C) | |
#Engineered | #Industrial | #Engineered product |
taxonmaps
- @ref: 69479
- File name: preview.99_4581.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_2285;97_2772;98_3442;99_4581&stattab=map
- Last taxonomy: Aureimonas frigidaquae
- 16S sequence: EF373540
- Sequence Identity:
- Total samples: 504
- soil counts: 94
- aquatic counts: 54
- animal counts: 330
- plant counts: 26
Safety information
risk assessment
- @ref: 16080
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16080
- description: Aurantimonas frigidaquae strain CW5 16S ribosomal RNA gene, partial sequence
- accession: EF373540
- length: 1378
- database: ena
- NCBI tax ID: 424757
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Aureimonas frigidaquae strain JCM 14755 | 424757.3 | wgs | patric | 424757 |
66792 | Aureimonas frigidaquae JCM 14755 | 2700989461 | draft | img | 424757 |
67770 | Aureimonas frigidaquae JCM 14755 | GCA_001463905 | contig | ncbi | 424757 |
GC content
@ref | GC-content | method |
---|---|---|
16080 | 63.9 | thermal denaturation, midpoint method (Tm) |
23136 | 63.9 | Thermal denaturation, fluorometry |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 96.938 | yes |
anaerobic | no | 97.97 | yes |
halophile | no | 90.96 | yes |
spore-forming | no | 96.4 | no |
glucose-util | yes | 89.486 | no |
aerobic | yes | 91.042 | no |
flagellated | no | 94.211 | no |
motile | no | 85.662 | no |
thermophile | no | 97.949 | yes |
glucose-ferment | no | 89.877 | yes |
External links
@ref: 16080
culture collection no.: DSM 21987, JCM 14755, KCTC 12893
straininfo link
- @ref: 70212
- straininfo: 353995
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 18450703 | Aurantimonas frigidaquae sp. nov., isolated from a water-cooling system. | Kim MS, Hoa KT, Baik KS, Park SC, Seong CN | Int J Syst Evol Microbiol | 10.1099/ijs.0.65421-0 | 2008 | Alphaproteobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Refrigeration, Sequence Analysis, DNA, Species Specificity | Genetics |
Phylogeny | 21186293 | Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina. | Rathsack K, Reitner J, Stackebrandt E, Tindall BJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.027029-0 | 2010 | Alphaproteobacteria/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
16080 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21987) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21987 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23136 | Mi Sun Kim, Kieu Thi Quynh Hoa, Keun Sik Baik, Seong Chan Park, Chi Nam Seong | 10.1099/ijs.0.65421-0 | Aurantimonas frigidaquae sp. nov., isolated from a water-cooling system | IJSEM 58: 1142-1146 2008 | 18450703 | |
23357 | K. Rathsack, J. Reitner, E. Stackebrandt, B. J. Tindall | 10.1099/ijs.0.027029-0 | Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina | IJSEM 61: 2722-2728 2011 | 21186293 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
70212 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID353995.1 | StrainInfo: A central database for resolving microbial strain identifiers |