Strain identifier

BacDive ID: 5334

Type strain: Yes

Species: Enterococcus asini

Strain history: CIP <- 2004, CCUG <- 2001, DSMZ <- C. Diviès <- A. de Vaux: strain AS2

NCBI tax ID(s): 57732 (species)

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General

@ref: 4387

BacDive-ID: 5334

DSM-Number: 11492

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus asini DSM 11492 is a microaerophile, mesophilic bacterium that was isolated from cecum of donkey.

NCBI tax id

  • NCBI tax id: 57732
  • Matching level: species

strain history

@refhistory
4387<- C. Diviès <- A. de Vaux, AS2
116528CIP <- 2004, CCUG <- 2001, DSMZ <- C. Diviès <- A. de Vaux: strain AS2

doi: 10.13145/bacdive5334.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus asini
  • full scientific name: Enterococcus asini de Vaux et al. 1998

@ref: 4387

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus asini

full scientific name: Enterococcus asini de Vaux et al. 1998

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.225
69480100positive
116528nopositivecoccus-shaped

colony morphology

  • @ref: 116528

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4387COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4387TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33922MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116528CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4387positivegrowth37mesophilic
33922positivegrowth37mesophilic
116528positivegrowth25-41
116528nogrowth10psychrophilic
116528nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4387microaerophile
116528facultative anaerobe

spore formation

@refspore formationconfidence
69481no96
69480no99.97

halophily

  • @ref: 116528
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 4387
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116528esculin+hydrolysis4853
116528hippurate+hydrolysis606565
116528nitrate-reduction17632
116528nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11652815688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
116528oxidase-
116528beta-galactosidase+3.2.1.23
116528alcohol dehydrogenase-1.1.1.1
116528gelatinase-
116528amylase-
116528catalase-1.11.1.6
116528gamma-glutamyltransferase-2.3.2.2
116528lysine decarboxylase-4.1.1.18
116528ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116528--++-+-+-+++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116528-----+---++++--------++++++++/---+/-------+---------+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
4387-+-------++-----+-+-++---+--+-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116528-+--+----++----+--+----------------------------------------+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4387cecum of donkeyFranceFRAEurope
56549Cecum of donkey
116528Donkey, caecum

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Gastrointestinal tract#Large intestine

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43871Risk group (German classification)
1165282Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4387
  • description: Enterococcus asini strain AS2 16S ribosomal RNA, partial sequence
  • accession: NR_029337
  • length: 1551
  • database: nuccore
  • NCBI tax ID: 57732

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus asini DSM 11492GCA_001885725scaffoldncbi57732
66792Enterococcus asini strain DSM 1149257732.3wgspatric57732

GC content

  • @ref: 4387
  • GC-content: 39

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
flagellatedno97.255no
gram-positiveyes94.63no
anaerobicno92.481yes
aerobicno96.973yes
halophileyes77.804no
spore-formingno91.185no
glucose-utilyes90.47no
thermophileno98.505no
motileno96.137no
glucose-fermentyes85.159no

External links

@ref: 4387

culture collection no.: DSM 11492, CCUG 44928, CIP 108239, AS 2

straininfo link

  • @ref: 74823
  • straininfo: 12430

literature

  • topic: Phylogeny
  • Pubmed-ID: 9731276
  • title: Enterococcus asini sp. nov. isolated from the caecum of donkeys (Equus asinus).
  • authors: de Vaux A, Laguerre G, Divies C, Prevost H
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-48-2-383
  • year: 1998
  • mesh: Animals, Base Composition, Base Sequence, Cecum/microbiology, DNA, Bacterial, Enterococcus/*classification/genetics, Equidae/*microbiology, Gram-Positive Bacterial Infections/microbiology/*veterinary, Molecular Sequence Data, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S/analysis, Sequence Analysis, RNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
4387Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11492)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11492
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33922Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5839
56549Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44928)https://www.ccug.se/strain?id=44928
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74823Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12430.1StrainInfo: A central database for resolving microbial strain identifiers
116528Curators of the CIPCollection of Institut Pasteur (CIP 108239)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108239