Strain identifier

BacDive ID: 5325

Type strain: Yes

Species: Enterococcus pseudoavium

Strain Designation: 47-16

Strain history: CIP <- 1993, NCFB <- 1979, M. Roguinsky: strain 47-16, Streptococcus bovis

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General

@ref: 2138

BacDive-ID: 5325

DSM-Number: 5632

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, animal pathogen

description: Enterococcus pseudoavium 47-16 is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from bovine mastitis.

NCBI tax id

NCBI tax idMatching level
1218092strain
44007species

strain history

@refhistory
2138<- NCDO <- M. Roguinsky, 47-16
67770ATCC 49372 <-- NCFB 2138 <-- M. Roguinsky.
121982CIP <- 1993, NCFB <- 1979, M. Roguinsky: strain 47-16, Streptococcus bovis

doi: 10.13145/bacdive5325.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus pseudoavium
  • full scientific name: Enterococcus pseudoavium Collins et al. 1989

@ref: 2138

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus pseudoavium

full scientific name: Enterococcus pseudoavium Collins et al. 1989

strain designation: 47-16

type strain: yes

Morphology

cell morphology

  • @ref: 121982
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 2138
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2138COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
2138TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34409MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
121982CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
2138positivegrowth37mesophilic
34409positivegrowth37mesophilic
51450positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
2138microaerophile
51450microaerophile
121982facultative anaerobe

murein

  • @ref: 2138
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121982nitrate-reduction17632
121982nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
12198235581indoleno
6838115688acetoinyes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
121982oxidase-
121982catalase-1.11.1.6
121982urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121982--++-+----++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121982----+--+-+++++---++-+++++++++--+------+-----+-+++

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
2138-+----++-++-----++-+/--++--+------
2138-+----++-+/-+-----++-+/--+---+------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
2138bovine mastitis
51450Bovine mastitisUnited KingdomGBREurope
67770Bovine mastitis
121982Animal, Bovine, mastitisFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Infection#Disease
#Infection#Inflammation
#Host Body-Site#Organ#Mammary gland

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
2138yes2Risk group (German classification)
1219822Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus pseudoavium strain ATCC 49372 16S ribosomal RNA gene, partial sequenceDQ4118091481ena44007
20218Enterococcus pseudoavium strain ATCC 49372 16S ribosomal RNA gene, partial sequenceKC510239815ena44007
2138Enterococcus pseudoavium 16S ribosomal RNA gene, partial sequenceAF0610021513ena44007
67770Enterococcus pseudoavium 16S rRNA gene, strain LMG 11426AJ3018371636ena44007
67770Enterococcus pseudoavium gene for 16S ribosomal RNA, partial sequence, strain: JCM 8732LC0970701454ena44007
67770Enterococcus pseudoavium 16S rRNA geneY183561424ena44007

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus pseudoavium NBRC 1004911218092.4wgspatric1218092
66792Enterococcus pseudoavium NBRC 1004912681812974draftimg1218092
67770Enterococcus pseudoavium NBRC 100491GCA_001544295contigncbi1218092

GC content

@refGC-contentmethod
213840.0thermal denaturation, midpoint method (Tm)
6777040thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.601no
gram-positiveyes89.378no
anaerobicno96.175no
halophileyes86.95no
spore-formingno92.595no
thermophileno99.529yes
glucose-utilyes89.407no
flagellatedno97.073no
aerobicno97.111yes
glucose-fermentyes85.763no

External links

@ref: 2138

culture collection no.: CCUG 33310, CIP 103647, DSM 5632, ATCC 49372, NCDO 2138, JCM 8732, CCM 4215, CECT 4075, CGMCC 1.2487, LMG 11426, NBRC 100491, NCFB 2138, NCIMB 13084, VTT E-97811

straininfo link

  • @ref: 74814
  • straininfo: 2269

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2138Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5632)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5632
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34409Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15468
51450Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33310)https://www.ccug.se/strain?id=33310
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
74814Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2269.1StrainInfo: A central database for resolving microbial strain identifiers
121982Curators of the CIPCollection of Institut Pasteur (CIP 103647)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103647