Strain identifier
BacDive ID: 5325
Type strain:
Species: Enterococcus pseudoavium
Strain Designation: 47-16
Strain history: CIP <- 1993, NCFB <- 1979, M. Roguinsky: strain 47-16, Streptococcus bovis
NCBI tax ID(s): 1218092 (strain), 44007 (species)
General
@ref: 2138
BacDive-ID: 5325
DSM-Number: 5632
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, animal pathogen
description: Enterococcus pseudoavium 47-16 is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from bovine mastitis.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1218092 | strain |
44007 | species |
strain history
@ref | history |
---|---|
2138 | <- NCDO <- M. Roguinsky, 47-16 |
67770 | ATCC 49372 <-- NCFB 2138 <-- M. Roguinsky. |
121982 | CIP <- 1993, NCFB <- 1979, M. Roguinsky: strain 47-16, Streptococcus bovis |
doi: 10.13145/bacdive5325.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus pseudoavium
- full scientific name: Enterococcus pseudoavium Collins et al. 1989
@ref: 2138
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus pseudoavium
full scientific name: Enterococcus pseudoavium Collins et al. 1989
strain designation: 47-16
type strain: yes
Morphology
cell morphology
- @ref: 121982
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 2138
- type of hemolysis: gamma
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2138 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
2138 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
34409 | MEDIUM 29- Brain heart agar | yes | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) | |
121982 | CIP Medium 29 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2138 | positive | growth | 37 | mesophilic |
34409 | positive | growth | 37 | mesophilic |
51450 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
2138 | microaerophile |
51450 | microaerophile |
121982 | facultative anaerobe |
murein
- @ref: 2138
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | + | builds acid from | |
68371 | gluconate | + | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | + | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | amygdalin | + | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | + | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | + | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | + | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | + | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
121982 | nitrate | - | reduction | 17632 |
121982 | nitrite | - | reduction | 16301 |
68381 | arginine | - | hydrolysis | 29016 |
68381 | D-ribose | + | builds acid from | 16988 |
68381 | D-mannitol | + | builds acid from | 16899 |
68381 | sorbitol | - | builds acid from | 30911 |
68381 | trehalose | + | builds acid from | 27082 |
68381 | raffinose | - | builds acid from | 16634 |
68381 | sucrose | - | builds acid from | 17992 |
68381 | L-arabinose | - | builds acid from | 30849 |
68381 | D-arabitol | - | builds acid from | 18333 |
68381 | alpha-cyclodextrin | - | builds acid from | 40585 |
68381 | glycogen | - | builds acid from | 28087 |
68381 | pullulan | - | builds acid from | 27941 |
68381 | maltose | + | builds acid from | 17306 |
68381 | melibiose | - | builds acid from | 28053 |
68381 | melezitose | - | builds acid from | 6731 |
68381 | methyl beta-D-glucopyranoside | - | builds acid from | 320055 |
68381 | D-tagatose | - | builds acid from | 16443 |
68381 | urea | - | hydrolysis | 16199 |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
121982 | 35581 | indole | no |
68381 | 15688 | acetoin | yes |
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68381 | urease | - | 3.5.1.5 |
68381 | beta-mannosidase | - | 3.2.1.25 |
68381 | glycyl tryptophan arylamidase | + | |
68381 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68381 | beta-galactosidase | - | 3.2.1.23 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | + | |
68381 | alkaline phosphatase | - | 3.1.3.1 |
68381 | alpha-galactosidase | - | 3.2.1.22 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | beta-glucosidase | + | 3.2.1.21 |
68381 | arginine dihydrolase | - | 3.5.3.6 |
121982 | oxidase | - | |
121982 | catalase | - | 1.11.1.6 |
121982 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121982 | - | - | + | + | - | + | - | - | - | - | + | + | - | - | - | + | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121982 | - | - | - | - | + | - | - | + | - | + | + | + | + | + | - | - | - | + | + | - | + | + | + | + | + | + | + | + | + | - | - | + | - | - | - | - | - | - | + | - | - | - | - | - | + | - | + | + | + |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2138 | - | + | - | - | - | - | + | + | - | + | + | - | - | - | - | - | + | + | - | +/- | - | + | + | - | - | + | - | - | - | - | - | - |
2138 | - | + | - | - | - | - | + | + | - | +/- | + | - | - | - | - | - | + | + | - | +/- | - | + | - | - | - | + | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
2138 | bovine mastitis | |||
51450 | Bovine mastitis | United Kingdom | GBR | Europe |
67770 | Bovine mastitis | |||
121982 | Animal, Bovine, mastitis | France | FRA | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Bovinae (Cow, Cattle) |
#Infection | #Disease | |
#Infection | #Inflammation | |
#Host Body-Site | #Organ | #Mammary gland |
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
2138 | yes | 2 | Risk group (German classification) |
121982 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Enterococcus pseudoavium strain ATCC 49372 16S ribosomal RNA gene, partial sequence | DQ411809 | 1481 | ena | 44007 |
20218 | Enterococcus pseudoavium strain ATCC 49372 16S ribosomal RNA gene, partial sequence | KC510239 | 815 | ena | 44007 |
2138 | Enterococcus pseudoavium 16S ribosomal RNA gene, partial sequence | AF061002 | 1513 | ena | 44007 |
67770 | Enterococcus pseudoavium 16S rRNA gene, strain LMG 11426 | AJ301837 | 1636 | ena | 44007 |
67770 | Enterococcus pseudoavium gene for 16S ribosomal RNA, partial sequence, strain: JCM 8732 | LC097070 | 1454 | ena | 44007 |
67770 | Enterococcus pseudoavium 16S rRNA gene | Y18356 | 1424 | ena | 44007 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Enterococcus pseudoavium NBRC 100491 | 1218092.4 | wgs | patric | 1218092 |
66792 | Enterococcus pseudoavium NBRC 100491 | 2681812974 | draft | img | 1218092 |
67770 | Enterococcus pseudoavium NBRC 100491 | GCA_001544295 | contig | ncbi | 1218092 |
GC content
@ref | GC-content | method |
---|---|---|
2138 | 40.0 | thermal denaturation, midpoint method (Tm) |
67770 | 40 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 95.601 | no |
gram-positive | yes | 89.378 | no |
anaerobic | no | 96.175 | no |
halophile | yes | 86.95 | no |
spore-forming | no | 92.595 | no |
thermophile | no | 99.529 | yes |
glucose-util | yes | 89.407 | no |
flagellated | no | 97.073 | no |
aerobic | no | 97.111 | yes |
glucose-ferment | yes | 85.763 | no |
External links
@ref: 2138
culture collection no.: CCUG 33310, CIP 103647, DSM 5632, ATCC 49372, NCDO 2138, JCM 8732, CCM 4215, CECT 4075, CGMCC 1.2487, LMG 11426, NBRC 100491, NCFB 2138, NCIMB 13084, VTT E-97811
straininfo link
- @ref: 74814
- straininfo: 2269
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2138 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5632) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5632 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
34409 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15468 | ||||
51450 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 33310) | https://www.ccug.se/strain?id=33310 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68381 | Automatically annotated from API rID32STR | |||||
68382 | Automatically annotated from API zym | |||||
74814 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID2269.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121982 | Curators of the CIP | Collection of Institut Pasteur (CIP 103647) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103647 |