Strain identifier

BacDive ID: 5317

Type strain: Yes

Species: Enterococcus malodoratus

Strain Designation: 6

Strain history: CIP <- 1987, NCFB <- 1956, T.E. Galesloot <- J.W. Pette: strain 6

NCBI tax ID(s): 1158601 (strain), 71451 (species)

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General

@ref: 9003

BacDive-ID: 5317

DSM-Number: 20681

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic

description: Enterococcus malodoratus 6 is a microaerophile, spore-forming, mesophilic bacterium that was isolated from gouda cheese.

NCBI tax id

NCBI tax idMatching level
1158601strain
71451species

strain history

@refhistory
9003<- NCDO <- E. Galesloot
67770ATCC 43197 <-- NCDO 846 <-- T. E. Galesloot 6.
120762CIP <- 1987, NCFB <- 1956, T.E. Galesloot <- J.W. Pette: strain 6

doi: 10.13145/bacdive5317.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus malodoratus
  • full scientific name: Enterococcus malodoratus (ex Pette 1955) Collins et al. 1984

@ref: 9003

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus malodoratus

full scientific name: Enterococcus malodoratus (ex Pette 1955) Collins et al. 1984

strain designation: 6

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no90.51
69480100positive
120762nopositivecoccus-shaped

colony morphology

  • @ref: 120762

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9003COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
9003TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40505MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
120762CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
9003positivegrowth37mesophilic
40505positivegrowth37mesophilic
50160positivegrowth37mesophilic
67770positivegrowth37mesophilic
120762positivegrowth10-41
120762nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
9003microaerophile
120762facultative anaerobe

spore formation

@refspore formationconfidence
69481yes92
69480no99.904

halophily

  • @ref: 120762
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 9003
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol+builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside+builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120762esculin+hydrolysis4853
120762hippurate-hydrolysis606565
120762nitrate-reduction17632
120762nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12076215688acetoin+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
120762oxidase-
120762beta-galactosidase+3.2.1.23
120762alcohol dehydrogenase-1.1.1.1
120762catalase-1.11.1.6
120762gamma-glutamyltransferase-2.3.2.2
120762lysine decarboxylase-4.1.1.18
120762ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120762--++-+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120762----+--+-++++++--+++-+++++++++++--+--+/-++-+--+++-+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
9003-+--++++/-+/-+++/-+---+++++++/---+++/-++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120762+++++-++--+++--+--++--+---++--+--+--+-------------------+--+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
9003gouda cheese
50160Cheese,Gouda
67770Gouda cheese
120762Food, Cheese, GoudaNetherlandsNLDEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
90031Risk group (German classification)
1207622Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus malodoratus 16S ribosomal RNA gene, partial sequenceAF0610121500ena71451
20218Enterococcus malodoratus strain ATCC 43197 16S ribosomal RNA gene, partial sequenceKC510237815ena71451
9003Enterococcus malodoratus 16S rRNA geneY183391461ena71451
67770Enterococcus malodoratus 16S rRNA gene, strain LMG 10747AJ3018351701ena71451
67770Enterococcus malodoratus gene for 16S ribosomal RNA, partial sequence, strain: JCM 8730LC0970681497ena71451

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus malodoratus ATCC 43197 [PRJNA202672]1158601.3wgspatric1158601
66792Enterococcus malodoratus ATCC 43197 [PRJNA206352]1158601.4wgspatric1158601
66792Enterococcus malodoratus strain DSM 2068171451.3wgspatric71451
66792Enterococcus malodoratus strain NCTC1236571451.7wgspatric71451
66792Enterococcus malodoratus ATCC 431972545824647draftimg1158601
66792Enterococcus malodoratus ATCC 431972558860908draftimg1158601
67770Enterococcus malodoratus ATCC 43197GCA_000393875scaffoldncbi1158601
67770Enterococcus malodoratus ATCC 43197GCA_000407185scaffoldncbi1158601
67770Enterococcus malodoratus DSM 20681GCA_001886025scaffoldncbi71451
67770Enterococcus malodoratus NCTC12365GCA_900447955contigncbi71451

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes92no
motileno93.845no
gram-positiveyes87.353no
anaerobicno93no
aerobicno96.074no
halophileyes70.235no
spore-formingno89.378no
flagellatedno96.856no
thermophileno99.776yes
glucose-utilyes88.18no
glucose-fermentyes86.175no

External links

@ref: 9003

culture collection no.: DSM 20681, ATCC 43197, NCDO 846, CCUG 30572, CCM 4056, JCM 8730, BCRC 17125, CECT 971, CGMCC 1.2493, CIP 103012, HAMBI 1569, KCTC 3641, LMG 10747, NBRC 100489, NCIMB 700846, NCTC 12365

straininfo link

  • @ref: 74806
  • straininfo: 2265

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9003Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20681)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20681
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40505Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14762
50160Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30572)https://www.ccug.se/strain?id=30572
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74806Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2265.1StrainInfo: A central database for resolving microbial strain identifiers
120762Curators of the CIPCollection of Institut Pasteur (CIP 103012)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103012