Strain identifier

BacDive ID: 531

Type strain: Yes

Species: Martelella mediterranea

Strain Designation: MACL11

Strain history: CIP <- 2005, CECT <- E. Velazquez, Salamanca Univ., Salamanca, Spain: strain MACL11

NCBI tax ID(s): 1122214 (strain), 293089 (species)

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General

@ref: 6894

BacDive-ID: 531

DSM-Number: 17316

keywords: genome sequence, 16S sequence, Bacteria, aerobe, halotolerant, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Martelella mediterranea MACL11 is an aerobe, halotolerant, mesophilic bacterium that forms circular colonies and was isolated from water sample from the subterranean saline Lake Martel .

NCBI tax id

NCBI tax idMatching level
293089species
1122214strain

strain history

@refhistory
6894<- R. Rivas, Depart. Microbiol. y Gen., Univ. Salamanca, Spain; MACL11
120873CIP <- 2005, CECT <- E. Velazquez, Salamanca Univ., Salamanca, Spain: strain MACL11

doi: 10.13145/bacdive531.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Rhizobiaceae
  • genus: Martelella
  • species: Martelella mediterranea
  • full scientific name: Martelella mediterranea Rivas et al. 2005

@ref: 6894

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Rhizobiaceae

genus: Martelella

species: Martelella mediterranea

full scientific name: Martelella mediterranea Rivas et al. 2005

strain designation: MACL11

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23133negative1.3-1.5 µm0.4-1.1 µmrod-shapedno
69480negative99.975
120873negativerod-shapedno

colony morphology

  • @ref: 23133
  • colony size: 1-3 mm
  • colony color: white to cream
  • colony shape: circular
  • incubation period: 5 days
  • medium used: YED medium

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_17316_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17316_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17316_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17316_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17316_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6894YED MEDIUM, SALTED (DSMZ Medium 1123)yeshttps://mediadive.dsmz.de/medium/1123Name: YED MEDIUM, salted (DSMZ Medium 1123) Composition: Agar 20.0 g/l NaCl 10.0 g/l Glucose 7.0 g/l Yeast extract 4.0 g/l Distilled water
23133YED mediumyes5% (w/v) NaCl
38297Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120873CIP Medium 343yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=343
120873CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6894positivegrowth28mesophilic
23133positivemaximum37mesophilic
23133positiveminimum4psychrophilic
23133positiveoptimum28mesophilic
38297positivegrowth30mesophilic
120873positivegrowth25-30mesophilic
120873nogrowth10psychrophilic
120873nogrowth37mesophilic
120873nogrowth41thermophilic
120873nogrowth45thermophilic

culture pH

@refabilitytypepHPH range
23133positiveminimum5.0
23133positivemaximum8.5alkaliphile
23133positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23133aerobe
120873obligate aerobe

spore formation

@refspore formationconfidence
23133no
69481no100
69480no99.992

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23133halotolerantNaClnogrowth10 %(w/v)
23133NaClpositivegrowth<5 %(w/v)
23133NaClpositiveoptimum2.0 %(w/v)
120873NaClpositivegrowth2-10 %
120873NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2313327897tryptophan-energy source
2313317128adipate-growth
2313327613amygdalin-growth
2313322599arabinose-growth
2313327689decanoate-growth
2313316947citrate-growth
2313317268myo-inositol-growth
2313337684mannose-growth
2313328053melibiose-growth
23133506227N-acetylglucosamine-growth
2313318401phenylacetate-growth
2313326546rhamnose-growth
2313330911sorbitol-growth
2313317992sucrose-growth
2313316136hydrogen sulfide-other
2313324265gluconate+carbon source
2313317234glucose+carbon source
2313325115malate+carbon source
2313317306maltose+carbon source
2313329864mannitol+carbon source
231334853esculin+hydrolysis
2313317632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837128847D-fucose-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837112936D-galactose-builds acid from
12087316947citrate-carbon source
1208734853esculin+hydrolysis
120873606565hippurate+hydrolysis
12087317632nitrate+reduction
12087316301nitrite-reduction
12087317632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
2313335581indoleno
2313315688acetoinyes
12087335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
2313315688acetoin+
2313335581indole-
12087315688acetoin-
12087317234glucose-

enzymes

@refvalueactivityec
23133acid phosphatase+3.1.3.2
23133alkaline phosphatase+3.1.3.1
23133alpha-fucosidase-3.2.1.51
23133alpha-galactosidase+3.2.1.22
23133alpha-mannosidase-3.2.1.24
23133arginine dihydrolase-3.5.3.6
23133beta-galactosidase+3.2.1.23
23133beta-glucuronidase+3.2.1.31
23133caseinase-3.4.21.50
23133chymotrypsin-3.4.4.5
23133cystine arylamidase+3.4.11.3
23133esterase (C 4)+
23133esterase lipase (C 8)+
23133gelatinase-
23133leucine arylamidase+3.4.11.1
23133lipase (C 14)+
23133lysine decarboxylase-4.1.1.18
23133N-acetyl-beta-glucosaminidase+3.2.1.52
23133naphthol-AS-BI-phosphohydrolase-
23133ornithine decarboxylase-4.1.1.17
23133trypsin+3.4.21.4
23133tryptophan deaminase-4.1.99.1
23133urease+3.5.1.5
23133valine arylamidase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120873oxidase+
120873beta-galactosidase+3.2.1.23
120873alcohol dehydrogenase-1.1.1.1
120873gelatinase-
120873amylase-
120873DNase-
120873caseinase-3.4.21.50
120873catalase+1.11.1.6
120873tween esterase-
120873gamma-glutamyltransferase+2.3.2.2
120873lecithinase-
120873lipase-
120873lysine decarboxylase-4.1.1.18
120873ornithine decarboxylase-4.1.1.17
120873phenylalanine ammonia-lyase-4.3.1.24
120873tryptophan deaminase-
120873urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120873-+++-+--+-++-+-+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120873+/-+/-+/-+/-+/-+/-+/-+/-+/--+/----+/---+/------+/-+/-+/-+/-+/-+/-+/-+/-+/---+/---+/-+/-+/-+/-+/--+/-+/-+/----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120873---+--+++-++-+-++-----+---++--++-+-+-+--++------++---------+----+--------+------+-------+--+-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6894water sample from the subterranean saline Lake Martel (at a depth of 10 cm)MallorcaSpainESPEurope
120873Environment, Water from a subterranean marine lakeMartel lake, MallorcaSpainESPEurope2003

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_1996.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_433;97_495;98_567;99_1996&stattab=map
  • Last taxonomy: Martelella
  • 16S sequence: AY649762
  • Sequence Identity:
  • Total samples: 2153
  • soil counts: 356
  • aquatic counts: 1132
  • animal counts: 514
  • plant counts: 151

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68941Risk group (German classification)
1208731Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6894
  • description: Martelella mediterranea strain MACL11 16S ribosomal RNA gene, partial sequence
  • accession: AY649762
  • length: 1474
  • database: ena
  • NCBI tax ID: 293089

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Martelella mediterranea DSM 17316 MACL11GCA_002043005completencbi1122214
66792Martelella mediterranea DSM 17316 MACL11GCA_000376125contigncbi1122214
66792Martelella mediterranea DSM 173161122214.3wgspatric1122214
66792Martelella mediterranea DSM 17316 strain MACL111122214.9plasmidpatric1122214
66792Martelella mediterranea DSM 17316 strain MACL111122214.8plasmidpatric1122214
66792Martelella mediterranea DSM 17316 strain MACL111122214.10plasmidpatric1122214
66792Martelella mediterranea DSM 173162515154079draftimg1122214

GC content

@refGC-contentmethod
689457.4
2313357.4Thermal denaturation, fluorometry

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno96.21yes
gram-positiveno97.27yes
anaerobicno99.176no
aerobicyes87.929no
halophileno76.415no
spore-formingno95.569yes
glucose-utilyes91.415yes
thermophileno97.176yes
motileno88.978yes
glucose-fermentno86.508no

External links

@ref: 6894

culture collection no.: DSM 17316, CECT 5861, LMG 22193, CIP 108716

straininfo link

  • @ref: 70209
  • straininfo: 128369

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15774691Martelella mediterranea gen. nov., sp. nov., a novel alpha-proteobacterium isolated from a subterranean saline lake.Rivas R, Sanchez-Marquez S, Mateos PF, Martinez-Molina E, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.63438-02005Aerobiosis, Alphaproteobacteria/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Spain, Species SpecificityGenetics
Phylogeny23355694Martelella endophytica sp. nov., an antifungal bacterium associated with a halophyte.Bibi F, Chung EJ, Khan A, Jeon CO, Chung YRInt J Syst Evol Microbiol10.1099/ijs.0.048785-02013Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rosa/*microbiology, Salt-Tolerant Plants/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/analysisGenetics
Phylogeny27393138Martelella suaedae sp. nov. and Martelella limonii sp. nov., isolated from the root of halophytes.Chung EJ, Hwang JM, Kim KH, Jeon CO, Chung YRInt J Syst Evol Microbiol10.1099/ijsem.0.0012882016Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Chenopodiaceae/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/*microbiology, Plumbaginaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Salt-Tolerant Plants/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistryTranscriptome
28983283The Composite 259-kb Plasmid of Martelella mediterranea DSM 17316(T)-A Natural Replicon with Functional RepABC Modules from Rhodobacteraceae and Rhizobiaceae.Bartling P, Brinkmann H, Bunk B, Overmann J, Goker M, Petersen JFront Microbiol10.3389/fmicb.2017.017872017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6894Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17316)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17316
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23133Raúl Rivas, Salud Sánchez-Márquez, Pedro F. Mateos, Eustoquio Martínez-Molina, Encarna Velázquez10.1099/ijs.0.63438-0Martelella mediterranea gen. nov., sp. nov., a novel a-proteobacterium isolated from a subterranean saline lakeIJSEM 55: 955-959 200515774691
38297Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6376
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70209Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID128369.1StrainInfo: A central database for resolving microbial strain identifiers
120873Curators of the CIPCollection of Institut Pasteur (CIP 108716)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108716