Strain identifier

BacDive ID: 5307

Type strain: No

Species: Enterococcus faecium

Strain history: <- National Culture Collection of Pathogens, South Korea; NCCP 12353 <- Korean National Institute of Health

NCBI tax ID(s): 1352 (species)

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General

@ref: 18121

BacDive-ID: 5307

DSM-Number: 25868

keywords: Bacteria, microaerophile, mesophilic

description: Enterococcus faecium DSM 25868 is a microaerophile, mesophilic bacterium that was isolated from human stool.

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history

  • @ref: 18121
  • history: <- National Culture Collection of Pathogens, South Korea; NCCP 12353 <- Korean National Institute of Health

doi: 10.13145/bacdive5307.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 18121

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

type strain: no

Morphology

colony morphology

  • @ref: 18121
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18121COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
18121TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 18121
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 18121
  • oxygen tolerance: microaerophile

antibiogram

  • @ref: 18121
  • medium: Mueller-Hinton Agar
  • incubation temperature: 37
  • incubation time: 1
  • oxygen condition: microaerophil
  • Penicillin G: 28
  • oxacillin: 20
  • ampicillin: 30
  • ticarcillin: 30
  • mezlocillin: 26
  • cefalotin: 22
  • cefazolin: 18
  • cefotaxime: 20
  • aztreonam: 0
  • imipenem: 28
  • tetracycline: 0
  • chloramphenicol: 24
  • gentamycin: 12-14
  • amikacin: 12
  • vancomycin: 20
  • erythromycin: 12
  • lincomycin: 0
  • ofloxacin: 14
  • norfloxacin: 14
  • colistin: 6
  • pipemidic acid: 6
  • nitrofurantoin: 16
  • bacitracin: 16
  • polymyxin b: 0
  • kanamycin: 6
  • neomycin: 8
  • doxycycline: 12
  • ceftriaxone: 20
  • clindamycin: 12-14
  • fosfomycin: 20
  • moxifloxacin: 16
  • linezolid: 30
  • nystatin: 0
  • quinupristin/dalfopristin: 10
  • teicoplanin: 18
  • piperacillin/tazobactam: 24-26

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
18121catalase+1.11.1.6
18121cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
18121+++-+-++-++-++-++-+++++--+--+-+-

Isolation, sampling and environmental information

isolation

  • @ref: 18121
  • sample type: human stool
  • geographic location: Gunsan
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human

Safety information

risk assessment

  • @ref: 18121
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 18121

culture collection no.: DSM 25868, NCCP 12353

straininfo link

  • @ref: 74797
  • straininfo: 401777

Reference

@idauthorscataloguedoi/urltitle
18121Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25868)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25868
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68381Automatically annotated from API rID32STR
74797Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401777.1StrainInfo: A central database for resolving microbial strain identifiers