Strain identifier

BacDive ID: 5297

Type strain: No

Species: Enterococcus faecium

Strain Designation: LRA 55 03 77

Strain history: <- ATCC; ATCC 35667 <- D. Androphy; LRA 55 03 77

NCBI tax ID(s): 1352 (species)

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General

@ref: 2509

BacDive-ID: 5297

DSM-Number: 6177

keywords: genome sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus faecium LRA 55 03 77 is a microaerophile, mesophilic bacterium of the family Enterococcaceae.

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history

  • @ref: 2509
  • history: <- ATCC; ATCC 35667 <- D. Androphy; LRA 55 03 77

doi: 10.13145/bacdive5297.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 2509

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

strain designation: LRA 55 03 77

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.045
69480100positive

colony morphology

  • @ref: 2509
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2509COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
2509TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 2509
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2509
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.916

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837028087glycogen-builds acid from
6837028017starch+builds acid from
6837016634raffinose-builds acid from
6837015443inulin-builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol-builds acid from
6837016899D-mannitol+builds acid from
6837030849L-arabinose+builds acid from
6837016988D-ribose+builds acid from
6837029016arginine+hydrolysis
683704853esculin+hydrolysis
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838118333D-arabitol-builds acid from
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68370
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68370
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
2509catalase+1.11.1.6
2509cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase-3.1.3.1
68370beta-galactosidase+3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370alpha-galactosidase-3.2.1.22
68370pyrrolidonyl arylamidase+3.4.19.3
68370beta-glucosidase+3.2.1.21

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYGbeta HEM
52567+-++--+-+++++-++--+--
52567++++--+-+++++-++--+-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
2509+++---++-++-++-++-++++---+--+/--+-
52567++----++-++-++-+--++-----+----+-
2509++----++-++-++-++-+++++--+----+/--
2509+++---++-++-++/---+-++-----+------

Safety information

risk assessment

  • @ref: 2509
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus faecium NCTC7176GCA_900447785contigncbi1352
66792Enterococcus faecium strain NCTC71761352.4068wgspatric1352

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes69no
motileno94.15no
gram-positiveyes95.611no
anaerobicno96.882yes
halophileyes94.117no
spore-formingno91.984no
glucose-utilyes89.474no
thermophileno99.681yes
flagellatedno98.539no
aerobicno94.076yes
glucose-fermentyes86.887no

External links

@ref: 2509

culture collection no.: CCUG 35172, NCTC 7176, DSM 6177, ATCC 35667

straininfo link

  • @ref: 74787
  • straininfo: 46000

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14607400Construction and use of a computerized DNA fingerprint database for lactic acid bacteria from silage.Chan RK, Wortman CR, Smiley BK, Hendrick CAJ Microbiol Methods10.1016/s0167-7012(03)00186-62003DNA Fingerprinting/*methods, DNA, Bacterial/chemistry/genetics/metabolism, *Databases, Nucleic Acid, Deoxyribonuclease EcoRI/metabolism, Electrophoresis, Agar Gel, Image Processing, Computer-Assisted, Lactobacillus/*classification/genetics, Phylogeny, Ribotyping, Silage/*microbiologyMetabolism
Enzymology25519527Effects of the probiotic Enterococcus faecium NCIMB 10415 on selected lactic acid bacteria and enterobacteria in co-culture.Starke IC, Zentek J, Vahjen WBenef Microbes10.3920/BM2014.00522015Animals, Coculture Techniques, Enterococcus/chemistry/genetics/*growth & development, Enterococcus faecium/chemistry/genetics/*growth & development, Gastrointestinal Tract/microbiology, Kinetics, Lactobacillus/chemistry/genetics/*growth & development, Probiotics/*chemistry, Swine
Metabolism28068600Design, synthesis and antibacterial evaluation of novel pleuromutilin derivatives possessing piperazine linker.Gao ML, Zeng J, Fang X, Luo J, Jin Z, Liu YH, Tang YZEur J Med Chem10.1016/j.ejmech.2017.01.0042017Anti-Bacterial Agents/*chemical synthesis/chemistry/metabolism/*pharmacology, Chemistry Techniques, Synthetic, Diterpenes/chemical synthesis/chemistry/metabolism/pharmacology, *Drug Design, Methicillin-Resistant Staphylococcus aureus/drug effects, Microbial Sensitivity Tests, Molecular Conformation, Molecular Docking Simulation, Piperazine, Piperazines/*chemistry, Polycyclic Compounds, Ribosome Subunits, Large, Bacterial/chemistry/metabolism, Structure-Activity RelationshipPathogenicity
Enzymology28326840A new benzophenone glycoside from the leaves of Mitracarpus villosus.Ngwoke KG, Orame N, Liu S, Okoye FBC, Daletos G, Proksch PNat Prod Res10.1080/14786419.2017.13067012017Animals, Anti-Bacterial Agents/isolation & purification/*pharmacology, Bacteria/drug effects, Benzophenones/isolation & purification/*pharmacology, Cell Line, Tumor, Glycosides/isolation & purification/*pharmacology, Mice, Microbial Sensitivity Tests, Molecular Structure, Nigeria, Phytochemicals/isolation & purification/pharmacology, Plant Leaves/chemistry, Plants, Medicinal/chemistry, Rubiaceae/*chemistryPhylogeny

Reference

@idauthorscataloguedoi/urltitle
2509Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6177)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6177
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
52567Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35172)https://www.ccug.se/strain?id=35172
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74787Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46000.1StrainInfo: A central database for resolving microbial strain identifiers