Strain identifier

BacDive ID: 5296

Type strain: No

Species: Enterococcus faecium

Strain Designation: DVG 8582, Kulmbach 2, 2

Strain history: CIP <- 2009, DSMZ <- T. Schiellesser, Kulmbach: strain 2

NCBI tax ID(s): 1352 (species)

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General

@ref: 1248

BacDive-ID: 5296

DSM-Number: 2918

keywords: Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Enterococcus faecium DVG 8582 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from uncooked sausage.

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history

@refhistory
1248<- T. Schliesser, Kulmbach strain 2 <- L. Leistner
119225CIP <- 2009, DSMZ <- T. Schiellesser, Kulmbach: strain 2

doi: 10.13145/bacdive5296.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 1248

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

strain designation: DVG 8582, Kulmbach 2, 2

type strain: no

Morphology

cell morphology

  • @ref: 119225
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 1248
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1248COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1248TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41825MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119225CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
119225CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328

culture temp

@refgrowthtypetemperaturerange
1248positivegrowth37mesophilic
41825positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
1248microaerophile
119225facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11922517632nitrate-reduction
11922516301nitrite-reduction
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from

metabolite production

  • @ref: 119225
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
1248catalase+1.11.1.6
1248cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
119225oxidase-
119225catalase-1.11.1.6
119225urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119225--++-+-+-++--+--+---

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
1248++----++-++-++--+-+++----+--+-+-
1248++----++-+/-+-++-++-+++++--+--+-+-
1248++----++-++-++-+--++/------++/--+-++
1248++----++--+-++--+-++/------++-+---

Isolation, sampling and environmental information

isolation

@refsample type
1248uncooked sausage
51301Sausage,uncooked
119225Sausage, uncooked

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
12482Risk group (German classification)
1192252Risk group (French classification)

External links

@ref: 1248

culture collection no.: DSM 2918, CIP 110050, WDCM 00088, CCUG 32985, CECT 4102, LMG 11397, NCIMB 12672, NCTC 13640

straininfo link

  • @ref: 74786
  • straininfo: 2258

Reference

@idauthorscataloguedoi/urltitle
1248Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2918)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2918
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41825Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7864
51301Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32985)https://www.ccug.se/strain?id=32985
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
74786Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2258.1StrainInfo: A central database for resolving microbial strain identifiers
119225Curators of the CIPCollection of Institut Pasteur (CIP 110050)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110050