Strain identifier

BacDive ID: 5277

Type strain: No

Species: Enterococcus casseliflavus

Strain history: <- National Culture Collection of Pathogens, South Korea; NCCP 11515 <- Korean National Institute of Health

NCBI tax ID(s): 37734 (species)

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General

@ref: 14778

BacDive-ID: 5277

DSM-Number: 25781

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-positive, antibiotic resistance

description: Enterococcus casseliflavus DSM 25781 is a microaerophile, mesophilic, Gram-positive bacterium that has multiple antibiotic resistances and was isolated from human stool.

NCBI tax id

  • NCBI tax id: 37734
  • Matching level: species

strain history

  • @ref: 14778
  • history: <- National Culture Collection of Pathogens, South Korea; NCCP 11515 <- Korean National Institute of Health

doi: 10.13145/bacdive5277.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus casseliflavus
  • full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

  • synonyms

    @refsynonym
    20215Streptococcus faecium subsp. casseliflavus
    20215Enterococcus flavescens
    20215Streptococcus casseliflavus

@ref: 14778

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus casseliflavus

full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

  • @ref: 14778
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
14778UREAPLASMA MEDIUM (DSMZ Medium 1096)yeshttps://mediadive.dsmz.de/medium/1096Name: UREAPLASMA MEDIUM (DSMZ Medium 1096) Composition: PPLO broth 14.6269 g/l Yeast extract 0.606965 g/l Urea 0.39801 g/l Fish-Sperm DNA 0.199005 g/l L-Cysteine HCl 0.0995024 g/l Phenol red 0.0199005 g/l Horse serum Isovitalex Distilled water
14778COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
14778TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 14778
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 14778
  • oxygen tolerance: microaerophile

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
14778Mueller-Hinton Agar371microaerophil22-24028-3024-26262018082434-36282016828-30102022-2408-1026-2814-1612161834-36012202632-340201022
14778Mueller-Hinton Agar37microaerophil20024-26202016-1812-14002032-34261814-16022-2410-1216180024-2614-161012-1416-1834001824-2630-320181020
14778Mueller-Hinton Agar37microaerophil20024-26202016-1812-14002032-34261814-16022-2410-1216180024-2614-161012-1416-1834001824-2630-320181020

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.941

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine+hydrolysis
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose+builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
14778catalase+1.11.1.6
14778cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
14778+++-+--+-+++++--+-++++---++-+-+-
14778+++-+-+/-+-+++++--+-++++---+++++/-+-

Isolation, sampling and environmental information

isolation

  • @ref: 14778
  • sample type: human stool
  • geographic location: Seoul
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human

Safety information

risk assessment

  • @ref: 14778
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Enterococcus casseliflavus strain FDAARGOS_1122
  • accession: 37734.152
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 37734

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes70no
gram-positiveyes89.612no
anaerobicno96.92yes
halophileyes70.289no
spore-formingno75.566no
glucose-utilyes88.855no
aerobicno95.338yes
motileyes51.447no
flagellatedno81.448no
thermophileno98.722no
glucose-fermentyes87.204no

External links

@ref: 14778

culture collection no.: DSM 25781, NCCP 11515

straininfo link

  • @ref: 74767
  • straininfo: 408126

Reference

@idauthorscataloguedoi/urltitle
14778Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25781)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25781
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74767Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID408126.1StrainInfo: A central database for resolving microbial strain identifiers