Strain identifier

BacDive ID: 5275

Type strain: No

Species: Enterococcus casseliflavus

Strain Designation: 32488

Strain history: <- Kiel, 32488 (Streptococcus faecium)

NCBI tax ID(s): 37734 (species)

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General

@ref: 8634

BacDive-ID: 5275

DSM-Number: 20382

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Enterococcus casseliflavus 32488 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from raw milk.

NCBI tax id

  • NCBI tax id: 37734
  • Matching level: species

strain history

  • @ref: 8634
  • history: <- Kiel, 32488 (Streptococcus faecium)

doi: 10.13145/bacdive5275.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus casseliflavus
  • full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

  • synonyms

    @refsynonym
    20215Streptococcus faecium subsp. casseliflavus
    20215Enterococcus flavescens
    20215Streptococcus casseliflavus

@ref: 8634

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus casseliflavus

full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984

strain designation: 32488

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8634COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8634TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 8634
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 8634
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.802

murein

  • @ref: 8634
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381beta-glucosidase+3.2.1.21

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8634+++++-++++++++--+-++++---++++++-
8634++--+/--+++/-+++++--+-++++---++-+++-
8634-+-+/-------------+--+++---+/-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8634raw milk
52000Raw milkKiel ?GermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Host#Mammals

Safety information

risk assessment

  • @ref: 8634
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Enterococcus casseliflavus strain FDAARGOS_1121
  • accession: 37734.150
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 37734

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno75no
gram-positiveyes88.054no
anaerobicno97.258yes
halophileyes67.077no
spore-formingno67.116no
glucose-utilyes85.918no
aerobicno95.127yes
motileyes54.348no
flagellatedno77.522no
thermophileno99.711no
glucose-fermentyes85.56no

External links

@ref: 8634

culture collection no.: CCUG 34231, DSM 20382

straininfo link

  • @ref: 74765
  • straininfo: 49620

Reference

@idauthorscataloguedoi/urltitle
8634Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20382)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20382
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
52000Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34231)https://www.ccug.se/strain?id=34231
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74765Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49620.1StrainInfo: A central database for resolving microbial strain identifiers