Strain identifier

BacDive ID: 5272

Type strain: No

Species: Enterococcus casseliflavus

Strain Designation: CA 2

Strain history: CIP <- 1993, R. Pompei, Cagliari Univ., Cagliari, Italy: strain Ca 2, Enterococcus flavescens

NCBI tax ID(s): 1158603 (strain), 37734 (species)

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General

@ref: 3126

BacDive-ID: 5272

DSM-Number: 7370

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Enterococcus casseliflavus CA 2 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from human sepsis.

NCBI tax id

NCBI tax idMatching level
1158603strain
37734species

strain history

@refhistory
327681993, R. Pompei, Cagliari, Italy: strain Ca 2, Enterococcus flavescens
3126<- CCM <- R. Pompei; CA 2 (Enterococcus flavescens)
117206CIP <- 1993, R. Pompei, Cagliari Univ., Cagliari, Italy: strain Ca 2, Enterococcus flavescens

doi: 10.13145/bacdive5272.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus casseliflavus
  • full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

  • synonyms

    @refsynonym
    20215Streptococcus faecium subsp. casseliflavus
    20215Enterococcus flavescens
    20215Streptococcus casseliflavus

@ref: 3126

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus casseliflavus

full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984

strain designation: CA 2

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
117206positivecoccus-shapedno

colony morphology

  • @ref: 117206

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
32768MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
3126COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
3126TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92
117206CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
3126positivegrowth37mesophilic
32768positivegrowth37mesophilic
50155positivegrowth37mesophilic
117206positivegrowth25-41
117206nogrowth10psychrophilic
117206nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3126microaerophile
50155aerobe
117206facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.944

halophily

  • @ref: 117206
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 3126
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
117206esculin+hydrolysis4853
117206hippurate+hydrolysis606565
117206nitrate-reduction17632
117206nitrite-reduction16301
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-glucose+fermentation17634
68379D-ribose-fermentation16988
68379D-xylose+fermentation65327
68379D-mannitol+fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087
68381arginine+hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose+builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11720615688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381pyrrolidonyl arylamidase+3.4.19.3
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
117206oxidase-
117206beta-galactosidase+3.2.1.23
117206alcohol dehydrogenase+1.1.1.1
117206catalase-1.11.1.6
117206gamma-glutamyltransferase-2.3.2.2
117206lysine decarboxylase-4.1.1.18
117206ornithine decarboxylase-4.1.1.17

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
50155-+++-++++---+-+++++--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
50155--++-+-+-+++-+-+++--
117206--++-+---+++++--++--

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
50155----+-+---++++-+--+--++++++++++++------+----------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
3126+++-+--+-+++++--+-++++---++-+-+-
50155+++-+--+-+++++--+-++++---++-+-+-
3126+++-+--+-+++++--+--+-----++-+-+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3126human sepsis
50155Human blood,sepsis
117206Human, Tip of an intraveinous cannulaItalyITAEurope

isolation source categories

Cat1Cat2
#Host#Human
#Infection#Patient
#Infection#Inflammation

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31262Risk group (German classification)
1172062Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus casseliflavus ATCC 49996GCA_000407405contigncbi1158603
66792Enterococcus casseliflavus strain FDAARGOS_99837734.149completepatric37734
66792Enterococcus flavescens ATCC 49996 [PRJNA202674]1158603.3wgspatric1158603
66792Enterococcus flavescens ATCC 49996 [PRJNA206349]1158603.5wgspatric1158603
66792Enterococcus casseliflavus ATCC 499962558860903draftimg1158603
66792Enterococcus casseliflavus ATCC 499962545824673draftimg1158603
66792Enterococcus casseliflavus ATCC 49996GCA_000393915scaffoldncbi1158603

GC content

  • @ref: 3126
  • GC-content: 42.0
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno75no
gram-positiveyes90.566no
anaerobicno95.941yes
halophileyes66.09no
spore-formingno70.821no
glucose-utilyes86.497no
aerobicno95.356no
motileno50no
flagellatedno79.778no
thermophileno99.34no
glucose-fermentyes87.278no

External links

@ref: 3126

culture collection no.: DSM 7370, ATCC 49996, CCM 4239, CCUG 30567, CIP 103525, CCM 439

straininfo link

  • @ref: 74762
  • straininfo: 13934

literature

  • topic: Phylogeny
  • Pubmed-ID: 1503970
  • title: Enterococcus flavescens sp. nov., a new species of enterococci of clinical origin.
  • authors: Pompei R, Berlutti F, Thaller MC, Ingianni A, Cortis G, Dainelli B
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-42-3-365
  • year: 1992
  • mesh: DNA, Bacterial/chemistry, Enterococcus/chemistry/*classification/genetics/metabolism, Fatty Acids/biosynthesis, Gram-Positive Bacterial Infections/microbiology, Humans, Nucleic Acid Hybridization, Sequence Homology, Nucleic Acid
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitle
3126Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7370)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7370
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32768Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15332
50155Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30567)https://www.ccug.se/strain?id=30567
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74762Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13934.1StrainInfo: A central database for resolving microbial strain identifiers
117206Curators of the CIPCollection of Institut Pasteur (CIP 103525)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103525