Strain identifier
BacDive ID: 5271
Type strain: 
Species: Enterococcus casseliflavus
Strain Designation: 491, H.3.1
Strain history: <- NCFB <- ATCC <- C.B. van Niel, H.3.1, motile enterococcus (Streptococcus faecium)
NCBI tax ID(s): 888066 (strain), 37734 (species)
version 8.1 (current version)
General
@ref: 1847
BacDive-ID: 5271
DSM-Number: 4841
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive
description: Enterococcus casseliflavus 491 is a microaerophile, mesophilic, Gram-positive bacterium of the family Enterococcaceae.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 888066 | strain |
| 37734 | species |
strain history
- @ref: 1847
- history: <- NCFB <- ATCC <- C.B. van Niel, H.3.1, motile enterococcus (Streptococcus faecium)
doi: 10.13145/bacdive5271.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus casseliflavus
- full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984
synonyms
@ref synonym 20215 Streptococcus faecium subsp. casseliflavus 20215 Enterococcus flavescens 20215 Streptococcus casseliflavus
@ref: 1847
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus casseliflavus
full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984
strain designation: 491, H.3.1
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1847 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 1847 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
- @ref: 1847
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1847
- oxygen tolerance: microaerophile
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.981
murein
- @ref: 1847
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68381 | 16899 | D-mannitol | + | builds acid from |
| 68381 | 30911 | sorbitol | - | builds acid from |
| 68381 | 16634 | raffinose | - | builds acid from |
| 68381 | 17992 | sucrose | + | builds acid from |
| 68381 | 30849 | L-arabinose | + | builds acid from |
| 68381 | 18333 | D-arabitol | - | builds acid from |
| 68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
| 68381 | 606565 | hippurate | - | hydrolysis |
| 68381 | 28087 | glycogen | - | builds acid from |
| 68381 | 27941 | pullulan | - | builds acid from |
| 68381 | 17306 | maltose | + | builds acid from |
| 68381 | 6731 | melezitose | - | builds acid from |
| 68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
| 68381 | 16443 | D-tagatose | - | builds acid from |
| 68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68381 | urease | - | 3.5.1.5 |
| 68381 | glycyl tryptophan arylamidase | + | |
| 68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
| 68381 | beta-galactosidase | + | 3.2.1.23 |
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
| 68381 | alkaline phosphatase | - | 3.1.3.1 |
| 68381 | alpha-galactosidase | + | 3.2.1.22 |
| 68381 | beta-glucuronidase | - | 3.2.1.31 |
| 68381 | beta-glucosidase | + | 3.2.1.21 |
API rID32STR
| @ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1847 | + | + | + | - | + | - | - | + | - | + | + | - | + | + | - | - | + | - | + | + | + | + | - | - | - | + | - | - | + | - | + | - |
| 1847 | - | + | - | - | + | - | + | + | - | + | + | - | + | + | - | - | + | - | + | + | + | + | - | - | - | + | + | - | + | - | + | - |
| 1847 | - | + | - | - | + | - | - | + | - | - | - | - | + | + | - | - | + | - | + | + | + | + | - | - | - | + | - | - | + | - | +/- | - |
Safety information
risk assessment
- @ref: 1847
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Enterococcus casseliflavus strain ATCC 12755 16S-23S ribosomal RNA intergenic spacer, partial sequence
- accession: AY351313
- length: 225
- database: ena
- NCBI tax ID: 888066
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Enterococcus casseliflavus ATCC 12755 | GCA_000191365 | scaffold | ncbi | 888066 |
| 66792 | Enterococcus casseliflavus ATCC 12755 | 888066.3 | wgs | patric | 888066 |
| 66792 | Enterococcus casseliflavus ATCC 12755 | 651324030 | draft | img | 888066 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 60 | no |
| motile | yes | 57.424 | no |
| flagellated | no | 79.037 | no |
| gram-positive | yes | 89.143 | no |
| anaerobic | no | 97.472 | no |
| aerobic | no | 95.674 | yes |
| halophile | yes | 71.678 | no |
| spore-forming | no | 72.001 | no |
| glucose-util | yes | 86.162 | no |
| thermophile | no | 99.175 | yes |
| glucose-ferment | yes | 87.066 | no |
External links
@ref: 1847
culture collection no.: DSM 4841, ATCC 12755, NCFB 2725
straininfo link
- @ref: 74761
- straininfo: 35649
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1847 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4841) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4841 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 68381 | Automatically annotated from API rID32STR | |||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 74761 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID35649.1 | StrainInfo: A central database for resolving microbial strain identifiers |