Strain identifier
BacDive ID: 5267
Type strain: 
Species: Vagococcus carniphilus
Strain history: CIP <- 2004, CCUG <- 2002, CDC
NCBI tax ID(s): 218144 (species)
version 8.1 (current version)
General
@ref: 6695
BacDive-ID: 5267
DSM-Number: 17031
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, coccus-shaped
description: Vagococcus carniphilus DSM 17031 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from Food, ground beef.
NCBI tax id
- NCBI tax id: 218144
- Matching level: species
strain history
| @ref | history |
|---|---|
| 6695 | <- CCUG <- P. Shewmaker & R.R. Facklam, CDC <- NARMS, CDC, Atlanta; CDC SS1714 |
| 117187 | CIP <- 2004, CCUG <- 2002, CDC |
doi: 10.13145/bacdive5267.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Vagococcus
- species: Vagococcus carniphilus
- full scientific name: Vagococcus carniphilus Shewmaker et al. 2004
@ref: 6695
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Vagococcus
species: Vagococcus carniphilus
full scientific name: Vagococcus carniphilus Shewmaker et al. 2004
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell shape | motility |
|---|---|---|---|
| 30074 | positive | coccus-shaped | yes |
| 117187 | positive | oval-shaped | no |
colony morphology
- @ref: 117187
pigmentation
- @ref: 30074
- production: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 6695 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 6695 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
| 37426 | MEDIUM 6 - Columbia agar with 10 % horse blood | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml) | |
| 117187 | CIP Medium 6 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6695 | positive | growth | 37 | mesophilic |
| 37426 | positive | growth | 37 | mesophilic |
| 57360 | positive | growth | 30-37 | mesophilic |
| 117187 | positive | growth | 25-41 | |
| 117187 | no | growth | 10 | psychrophilic |
| 117187 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 6695 | microaerophile |
| 6695 | aerobe |
| 57360 | aerobe |
| 117187 | facultative anaerobe |
halophily
- @ref: 117187
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 6.5 %
observation
- @ref: 30074
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30074 | 17754 | glycerol | + | carbon source |
| 30074 | 17306 | maltose | + | carbon source |
| 30074 | 15361 | pyruvate | + | carbon source |
| 30074 | 33942 | ribose | + | carbon source |
| 30074 | 17992 | sucrose | + | carbon source |
| 30074 | 27082 | trehalose | + | carbon source |
| 30074 | 4853 | esculin | + | hydrolysis |
| 117187 | 4853 | esculin | + | hydrolysis |
| 117187 | 606565 | hippurate | + | hydrolysis |
| 117187 | 17632 | nitrate | - | reduction |
| 117187 | 16301 | nitrite | + | reduction |
| 117187 | 17632 | nitrate | + | respiration |
antibiotic resistance
- @ref: 117187
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite tests
- @ref: 117187
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 117187 | oxidase | - | |
| 117187 | beta-galactosidase | - | 3.2.1.23 |
| 117187 | alcohol dehydrogenase | - | 1.1.1.1 |
| 117187 | catalase | - | 1.11.1.6 |
| 117187 | gamma-glutamyltransferase | - | 2.3.2.2 |
| 117187 | lysine decarboxylase | - | 4.1.1.18 |
| 117187 | ornithine decarboxylase | - | 4.1.1.17 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | sampling date | isolation date |
|---|---|---|---|---|---|---|---|
| 6695 | Food, ground beef | Oregon | USA | USA | North America | ||
| 57360 | Food,ground beef | Oregon | USA | USA | North America | 2000 | |
| 117187 | Food, Ground beef | Oregon | United States of America | USA | North America | 2000 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Food production | #Meat |
| #Host | #Mammals | #Bovinae (Cow, Cattle) |
taxonmaps
- @ref: 69479
- File name: preview.99_12024.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_1881;97_2259;98_9029;99_12024&stattab=map
- Last taxonomy: Vagococcus fluvialis
- 16S sequence: AY179329
- Sequence Identity:
- Total samples: 9217
- soil counts: 1044
- aquatic counts: 1703
- animal counts: 6217
- plant counts: 253
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 6695 | 1 | Risk group (German classification) |
| 117187 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 6695
- description: Vagococcus carniphilus 16S ribosomal RNA gene, partial sequence
- accession: AY179329
- length: 1513
- database: ena
- NCBI tax ID: 218144
GC content
- @ref: 6695
- GC-content: 34
External links
@ref: 6695
culture collection no.: DSM 17031, ATCC BAA 640, CCUG 46823, CIP 108561, CDC SS1714, CCM 8414
straininfo link
- @ref: 74757
- straininfo: 97613
literature
- topic: Phylogeny
- Pubmed-ID: 15388702
- title: Vagococcus carniphilus sp. nov., isolated from ground beef.
- authors: Shewmaker PL, Steigerwalt AG, Morey RE, Carvalho MDGS, Elliott JA, Joyce K, Barrett TJ, Teixeira LM, Facklam RR
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02908-0
- year: 2004
- mesh: Animals, Bacterial Proteins/analysis/isolation & purification, Bacterial Typing Techniques, Cattle, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Electrophoresis, Polyacrylamide Gel, Enterococcus/classification, Esters, Food Microbiology, Genes, rRNA, Gram-Positive Bacteria/*classification/genetics/*isolation & purification, Meat/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oligopeptides, Oregon, Phylogeny, Proteome/analysis/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Enzymology
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 6695 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17031) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17031 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 30074 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26432 | 28776041 | ||
| 37426 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6197 | |||||
| 57360 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 46823) | https://www.ccug.se/strain?id=46823 | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 74757 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID97613.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
| 117187 | Curators of the CIP | Collection of Institut Pasteur (CIP 108561) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108561 |