Strain identifier
BacDive ID: 525
Type strain:
Species: Thermocrinis minervae
Strain Designation: CR11
Strain history: <- A.-L. Reysenbach, Dept. Biology, Portland State Univ., USA; CR11
NCBI tax ID(s): 381751 (species)
General
@ref: 8174
BacDive-ID: 525
DSM-Number: 19557
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, chemolithotroph, thermophilic, Gram-negative, motile, rod-shaped
description: Thermocrinis minervae CR11 is a microaerophile, chemolithotroph, thermophilic bacterium that was isolated from terrestrial hot spring.
NCBI tax id
- NCBI tax id: 381751
- Matching level: species
strain history
- @ref: 8174
- history: <- A.-L. Reysenbach, Dept. Biology, Portland State Univ., USA; CR11
doi: 10.13145/bacdive525.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/aquificota
- domain: Bacteria
- phylum: Aquificota
- class: Aquificia
- order: Aquificales
- family: Aquificaceae
- genus: Thermocrinis
- species: Thermocrinis minervae
- full scientific name: Thermocrinis minervae Caldwell et al. 2010
@ref: 8174
domain: Bacteria
phylum: Aquificae
class: Aquificae
order: Aquificales
family: Aquificaceae
genus: Thermocrinis
species: Thermocrinis minervae
full scientific name: Thermocrinis minervae Caldwell et al. 2010
strain designation: CR11
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
23130 | negative | 2.4-3.9 µm | 0.5-0.6 µm | rod-shaped | yes | |
69480 | negative | 99.999 |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
66793 | EM_DSM_19557_1.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_19557_2.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_19557_3.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_19557_4.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_19557_5.jpg | electron microscopic image | © HZI/Manfred Rohde |
Culture and growth conditions
culture medium
- @ref: 8174
- name: MS-MEDIUM (MODIFIED) (DSMZ Medium 1145)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1145
- composition: Name: MS-MEDIUM (MODIFIED) (DSMZ Medium 1145) Composition: MgSO4 x 7 H2O 7.0 g/l Elemental sulphur 5.0 g/l MgCl2 x 6 H2O 2.0 g/l NH4Cl 2.0 g/l NaS2O3 x 5 H2O 2.0 g/l CaCl2 x 2 H2O 0.8 g/l NaOH 0.79994 g/l KCl 0.48 g/l K2HPO4 x 3 H2O 0.3 g/l Na2-EDTA x 2 H2O 0.005 g/l CoCl2 x 6 H2O 0.0015 g/l ZnCl2 0.001 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l AlCl3 x 6 H2O 0.0004 g/l Na2WO4 x 2 H2O 0.0003 g/l CuCl2 x 2 H2O 0.0002 g/l NiSO4 x 6 H2O 0.0002 g/l H3BO3 0.0001 g/l H2SeO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Double distilled water Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
8174 | positive | growth | 75 | thermophilic | |
23130 | positive | growth | 65-85 | hyperthermophilic | |
23130 | positive | optimum | 75 | thermophilic | |
69480 | thermophilic | 100 |
culture pH
@ref | ability | type | pH |
---|---|---|---|
23130 | positive | minimum | 4.8 |
23130 | positive | maximum | 7.8 |
23130 | positive | growth | 4.8-7.8 |
23130 | positive | optimum | 5.9 |
Physiology and metabolism
oxygen tolerance
- @ref: 8174
- oxygen tolerance: microaerophile
nutrition type
- @ref: 23130
- type: chemolithotroph
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.908
halophily
- @ref: 23130
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-0.4 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23130 | 73706 | bromosuccinate | + | carbon source |
23130 | casamino acids | + | carbon source | |
23130 | 16947 | citrate | + | carbon source |
23130 | 17234 | glucose | + | carbon source |
23130 | 17306 | maltose | + | carbon source |
23130 | 37684 | mannose | + | carbon source |
23130 | peptone | + | carbon source | |
23130 | 28017 | starch | + | carbon source |
23130 | yeast extract | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 8174
- sample type: terrestrial hot spring
- geographic location: Rincón de la Vieja volcano, south-western foothills
- country: Costa Rica
- origin.country: CRI
- continent: Middle and South America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Environmental | #Terrestrial | |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_4978.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15840;96_2439;97_2972;98_3722;99_4978&stattab=map
- Last taxonomy: Thermocrinis minervae subclade
- 16S sequence: AM260555
- Sequence Identity:
- Total samples: 58
- soil counts: 3
- aquatic counts: 47
- animal counts: 8
Safety information
risk assessment
- @ref: 8174
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 8174
- description: Thermocrinis minervae partial 16S rRNA gene, strain CR11
- accession: AM260555
- length: 1491
- database: ena
- NCBI tax ID: 381751
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermocrinis minervae DSM 19557 | GCA_900142435 | chromosome | ncbi | 381751 |
66792 | Thermocrinis minervae strain DSM 19557 | 381751.3 | complete | patric | 381751 |
66792 | Thermocrinis minervae DSM 19557 | 2698536703 | draft | img | 381751 |
GC content
@ref | GC-content | method |
---|---|---|
8174 | 40.3 | thermal denaturation, midpoint method (Tm) |
23130 | 40.3 | Thermal denaturation, fluorometry |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 51 | no |
gram-positive | no | 99.491 | no |
anaerobic | no | 58.552 | yes |
halophile | no | 90.96 | no |
spore-forming | no | 96.881 | no |
glucose-util | yes | 60.246 | yes |
aerobic | no | 94.762 | yes |
motile | yes | 89.199 | yes |
flagellated | no | 91.924 | no |
thermophile | yes | 100 | yes |
glucose-ferment | no | 87.12 | no |
External links
@ref: 8174
culture collection no.: DSM 19557, ATCC BAA 1533
straininfo link
- @ref: 70203
- straininfo: 405654
literature
- topic: Phylogeny
- Pubmed-ID: 19651724
- title: Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring.
- authors: Caldwell SL, Liu Y, Ferrera I, Beveridge T, Reysenbach AL
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.010496-0
- year: 2009
- mesh: Base Composition, Costa Rica, DNA, Bacterial/chemistry, Gram-Negative Bacteria/*classification/genetics/metabolism, Hot Springs/*microbiology, Hydrogen/metabolism, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Bacterial/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA, Sulfur/metabolism, *Water Microbiology
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8174 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19557) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19557 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23130 | Sara L. Caldwell, Yitai Liu, Isabel Ferrera, Terry Beveridge, Anna-Louise Reysenbach | 10.1099/ijs.0.010496-0 | Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring | IJSEM 60: 338-343 2010 | 19651724 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
70203 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405654.1 | StrainInfo: A central database for resolving microbial strain identifiers |