Strain identifier

BacDive ID: 525

Type strain: Yes

Species: Thermocrinis minervae

Strain Designation: CR11

Strain history: <- A.-L. Reysenbach, Dept. Biology, Portland State Univ., USA; CR11

NCBI tax ID(s): 381751 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8174

BacDive-ID: 525

DSM-Number: 19557

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, chemolithotroph, thermophilic, Gram-negative, motile, rod-shaped

description: Thermocrinis minervae CR11 is a microaerophile, chemolithotroph, thermophilic bacterium that was isolated from terrestrial hot spring.

NCBI tax id

  • NCBI tax id: 381751
  • Matching level: species

strain history

  • @ref: 8174
  • history: <- A.-L. Reysenbach, Dept. Biology, Portland State Univ., USA; CR11

doi: 10.13145/bacdive525.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/aquificota
  • domain: Bacteria
  • phylum: Aquificota
  • class: Aquificia
  • order: Aquificales
  • family: Aquificaceae
  • genus: Thermocrinis
  • species: Thermocrinis minervae
  • full scientific name: Thermocrinis minervae Caldwell et al. 2010

@ref: 8174

domain: Bacteria

phylum: Aquificae

class: Aquificae

order: Aquificales

family: Aquificaceae

genus: Thermocrinis

species: Thermocrinis minervae

full scientific name: Thermocrinis minervae Caldwell et al. 2010

strain designation: CR11

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23130negative2.4-3.9 µm0.5-0.6 µmrod-shapedyes
69480negative99.999

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_19557_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_19557_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_19557_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_19557_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_19557_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 8174
  • name: MS-MEDIUM (MODIFIED) (DSMZ Medium 1145)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1145
  • composition: Name: MS-MEDIUM (MODIFIED) (DSMZ Medium 1145) Composition: MgSO4 x 7 H2O 7.0 g/l Elemental sulphur 5.0 g/l MgCl2 x 6 H2O 2.0 g/l NH4Cl 2.0 g/l NaS2O3 x 5 H2O 2.0 g/l CaCl2 x 2 H2O 0.8 g/l NaOH 0.79994 g/l KCl 0.48 g/l K2HPO4 x 3 H2O 0.3 g/l Na2-EDTA x 2 H2O 0.005 g/l CoCl2 x 6 H2O 0.0015 g/l ZnCl2 0.001 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l AlCl3 x 6 H2O 0.0004 g/l Na2WO4 x 2 H2O 0.0003 g/l CuCl2 x 2 H2O 0.0002 g/l NiSO4 x 6 H2O 0.0002 g/l H3BO3 0.0001 g/l H2SeO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Double distilled water Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
8174positivegrowth75thermophilic
23130positivegrowth65-85hyperthermophilic
23130positiveoptimum75thermophilic
69480thermophilic100

culture pH

@refabilitytypepH
23130positiveminimum4.8
23130positivemaximum7.8
23130positivegrowth4.8-7.8
23130positiveoptimum5.9

Physiology and metabolism

oxygen tolerance

  • @ref: 8174
  • oxygen tolerance: microaerophile

nutrition type

  • @ref: 23130
  • type: chemolithotroph

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.908

halophily

  • @ref: 23130
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-0.4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2313073706bromosuccinate+carbon source
23130casamino acids+carbon source
2313016947citrate+carbon source
2313017234glucose+carbon source
2313017306maltose+carbon source
2313037684mannose+carbon source
23130peptone+carbon source
2313028017starch+carbon source
23130yeast extract+carbon source

Isolation, sampling and environmental information

isolation

  • @ref: 8174
  • sample type: terrestrial hot spring
  • geographic location: Rincón de la Vieja volcano, south-western foothills
  • country: Costa Rica
  • origin.country: CRI
  • continent: Middle and South America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Environmental#Terrestrial
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4978.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15840;96_2439;97_2972;98_3722;99_4978&stattab=map
  • Last taxonomy: Thermocrinis minervae subclade
  • 16S sequence: AM260555
  • Sequence Identity:
  • Total samples: 58
  • soil counts: 3
  • aquatic counts: 47
  • animal counts: 8

Safety information

risk assessment

  • @ref: 8174
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8174
  • description: Thermocrinis minervae partial 16S rRNA gene, strain CR11
  • accession: AM260555
  • length: 1491
  • database: ena
  • NCBI tax ID: 381751

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermocrinis minervae DSM 19557GCA_900142435chromosomencbi381751
66792Thermocrinis minervae strain DSM 19557381751.3completepatric381751
66792Thermocrinis minervae DSM 195572698536703draftimg381751

GC content

@refGC-contentmethod
817440.3thermal denaturation, midpoint method (Tm)
2313040.3Thermal denaturation, fluorometry

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno51no
gram-positiveno99.491no
anaerobicno58.552yes
halophileno90.96no
spore-formingno96.881no
glucose-utilyes60.246yes
aerobicno94.762yes
motileyes89.199yes
flagellatedno91.924no
thermophileyes100yes
glucose-fermentno87.12no

External links

@ref: 8174

culture collection no.: DSM 19557, ATCC BAA 1533

straininfo link

  • @ref: 70203
  • straininfo: 405654

literature

  • topic: Phylogeny
  • Pubmed-ID: 19651724
  • title: Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring.
  • authors: Caldwell SL, Liu Y, Ferrera I, Beveridge T, Reysenbach AL
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.010496-0
  • year: 2009
  • mesh: Base Composition, Costa Rica, DNA, Bacterial/chemistry, Gram-Negative Bacteria/*classification/genetics/metabolism, Hot Springs/*microbiology, Hydrogen/metabolism, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Bacterial/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA, Sulfur/metabolism, *Water Microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8174Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19557)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19557
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23130Sara L. Caldwell, Yitai Liu, Isabel Ferrera, Terry Beveridge, Anna-Louise Reysenbach10.1099/ijs.0.010496-0Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot springIJSEM 60: 338-343 201019651724
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
70203Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405654.1StrainInfo: A central database for resolving microbial strain identifiers