Strain identifier

BacDive ID: 5242

Type strain: Yes

Species: Cronobacter muytjensii

Strain Designation: 83-07-023, E603, LRA 023 07 83

Strain history: CIP <- 1992, R. Mercier, bioMérieux, La Balme-les-Grottes, France, Enterobacter sakazakii: strain LRA 023 07 83 <- 1983, INSERM, Villeneuve d'Ascq, France

NCBI tax ID(s): 1159613 (strain), 413501 (species)

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General

@ref: 16003

BacDive-ID: 5242

DSM-Number: 21870

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Cronobacter muytjensii 83-07-023 is an aerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

NCBI tax idMatching level
413501species
1159613strain

strain history

@refhistory
16003<- CIP <- R. Mercier, bioMérieux, La Balme-les-Grottes, France; LRA 023 07 83 (Enterobacter sakazakii) <- INSERM, Villeneuve d'Ascq
121207CIP <- 1992, R. Mercier, bioMérieux, La Balme-les-Grottes, France, Enterobacter sakazakii: strain LRA 023 07 83 <- 1983, INSERM, Villeneuve d'Ascq, France

doi: 10.13145/bacdive5242.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Cronobacter
  • species: Cronobacter muytjensii
  • full scientific name: Cronobacter muytjensii Iversen et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Cronobacter muytjensii

@ref: 16003

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Cronobacter

species: Cronobacter muytjensii

full scientific name: Cronobacter muytjensii Iversen et al. 2008

strain designation: 83-07-023, E603, LRA 023 07 83

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32494negative3 µm1 µmrod-shapedyes
69480yes95.483
69480negative99.865
121207negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16003TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
41817MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
16003TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
16003COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
121207CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
121207CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
16003positivegrowth28mesophilic
32494positivegrowth06-45
41817positivegrowth30mesophilic
16003positivegrowth30mesophilic

culture pH

  • @ref: 32494
  • ability: positive
  • type: growth
  • pH: 05-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32494aerobe
121207facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.101

halophily

  • @ref: 32494
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3249415792malonate+carbon source
3249417268myo-inositol+carbon source
3249417148putrescine+carbon source
324944853esculin+hydrolysis
3249417632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
12120717632nitrate+reduction
12120716301nitrite+reduction

metabolite production

@refChebi-IDmetaboliteproduction
3249435581indoleyes
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno
12120735581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
3249435581indole+
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
32494catalase+1.11.1.6
68368cytochrome oxidase-1.9.3.1
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
121207oxidase-
121207catalase+1.11.1.6
121207urease-3.5.1.5

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
16003++-++---++++++-+++++-
16003++-++---++-+++-+++++-

Isolation, sampling and environmental information

isolation

  • @ref: 16003
  • country: France
  • origin.country: FRA
  • continent: Europe

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
160032Risk group (German classification)
1212072Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterobacter sakazakii partial 16S-23S IGS, strain ATCC 51329AM295804154ena28141
20218Enterobacter sakazakii strain ATCC 51329 16S ribosomal RNA gene, partial sequenceAY579153528ena28141
20218Enterobacter sakazakii strain ATCC 51329 16S ribosomal RNA gene, partial sequenceAY7529371480ena28141
20218Cronobacter muytjensii strain ATCC 51329 16S ribosomal RNA gene, partial sequenceGU1222171507ena1159613
20218Cronobacter muytjensii strain ATCC 51329 16S ribosomal RNA gene, partial sequenceHM0692261413ena1159613
20218Cronobacter muytjensii strain ATCC 51329 16S ribosomal RNA gene, partial sequenceHQ8804211443ena1159613
16003Cronobacter muytjensii ATCC 51329 strain E603 16S ribosomal RNA gene, partial sequenceEF0598451425ena1159613

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cronobacter muytjensii ATCC 51329GCA_001277195completencbi1159613
66792Cronobacter muytjensii ATCC 51329GCA_000409285contigncbi1159613
66792Cronobacter muytjensii ATCC 513291159613.6completepatric1159613
66792Cronobacter muytjensii ATCC 513291159613.3wgspatric1159613
66792Cronobacter muytjensii ATCC 513292548876993draftimg1159613
66792Cronobacter muytjensii ATCC 513292667527495completeimg1159613

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes91.855no
flagellatedno77.763no
gram-positiveno98.059no
anaerobicno97.054yes
halophileno71.428yes
spore-formingno93.991no
thermophileno99.056yes
glucose-utilyes95.71no
aerobicyes82.492yes
glucose-fermentyes91.534no

External links

@ref: 16003

culture collection no.: DSM 21870, ATCC 51329, CIP 103581, WDCM 00213, CECT 9143

straininfo link

  • @ref: 74733
  • straininfo: 42926

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18523192Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten HInt J Syst Evol Microbiol10.1099/ijs.0.65577-02008Bacterial Typing Techniques, Cronobacter sakazakii/*classification/genetics/metabolism/physiology, DNA, Bacterial/analysis, Enterobacteriaceae/*classification/genetics/metabolism/physiology, Genes, rRNA, Malonates/metabolism, Microarray Analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Reagent Kits, Diagnostic, Sequence Analysis, DNA, Species SpecificityMetabolism
Enzymology31521366Plasmonic ELISA based on DNA-directed gold nanoparticle growth for Cronobacter detection in powdered infant formula samples.Wu Y, Xiong Y, Chen X, Luo D, Gao B, Chen J, Huang X, Leng Y, Xiong YJ Dairy Sci10.3168/jds.2019-170672019Cronobacter/genetics/*isolation & purification, Cronobacter sakazakii/genetics, DNA, Bacterial/isolation & purification, Enzyme-Linked Immunosorbent Assay/*methods, Food Microbiology, Food Safety, *Gold/chemistry, Humans, Hydrogen Peroxide, Infant Formula/*microbiology, Iron, *Metal Nanoparticles/chemistry, PowdersPhylogeny
Phylogeny31675291Mixta tenebrionis sp. nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.Xia M, Wang J, Huo YX, Yang YInt J Syst Evol Microbiol10.1099/ijsem.0.0038262020Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/isolation & purification, Gastrointestinal Tract/*microbiology, Nucleic Acid Hybridization, *Phylogeny, *Plastics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tenebrio/*microbiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
16003Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21870)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21870
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32494Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128714
41817Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15394
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74733Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42926.1StrainInfo: A central database for resolving microbial strain identifiers
121207Curators of the CIPCollection of Institut Pasteur (CIP 103581)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103581