Strain identifier
BacDive ID: 524
Type strain: 
Species: Thermocrinis albus
Strain Designation: HI 11/12
Strain history: W. Eder and K. O. Stetter HI 11/12.
NCBI tax ID(s): 638303 (strain), 136094 (species)
version 8.1 (current version)
General
@ref: 5375
BacDive-ID: 524
DSM-Number: 14484
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, hyperthermophilic, Gram-negative
description: Thermocrinis albus HI 11/12 is a microaerophile, hyperthermophilic, Gram-negative bacterium that was isolated from whitisch steamers of the Hveragerthi area.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 136094 | species |
| 638303 | strain |
strain history
| @ref | history |
|---|---|
| 5375 | <- W. Eder, Univ. Regensburg; HI 11/12 <- W. Eder and R. Huber |
| 67770 | W. Eder and K. O. Stetter HI 11/12. |
doi: 10.13145/bacdive524.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/aquificota
- domain: Bacteria
- phylum: Aquificota
- class: Aquificia
- order: Aquificales
- family: Aquificaceae
- genus: Thermocrinis
- species: Thermocrinis albus
- full scientific name: Thermocrinis albus Eder and Huber 2002
@ref: 5375
domain: Bacteria
phylum: Aquificae
class: Aquificae
order: Aquificales
family: Aquificaceae
genus: Thermocrinis
species: Thermocrinis albus
full scientific name: Thermocrinis albus Eder and Huber 2002
strain designation: HI 11/12
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.999
multimedia
- @ref: 66793
- multimedia content: EM_DSM_14484_1.jpg
- caption: electron microscopic image
- intellectual property rights: © HZI/Manfred Rohde
Culture and growth conditions
culture medium
- @ref: 5375
- name: OS MEDIUM (DSMZ Medium 887)
- growth: yes
- link: https://mediadive.dsmz.de/medium/887
- composition: Name: OS MEDIUM (DSMZ Medium 887) Composition: Gelrite 15.0 g/l NaHCO3 1.0 g/l Na2S2O3 x 5 H2O 1.0 g/l MgSO4 x 7 H2O 0.03 g/l Na2SO4 0.023 g/l Nitrilotriacetic acid 0.015 g/l KCl 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l KH2PO4 0.0017 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l NaNO3 0.0003 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l FeCl3 x 6 H2O 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l MnSO4 x 4 H2O 6e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 5375 | positive | growth | 80 | hyperthermophilic | |
| 67770 | positive | growth | 80 | hyperthermophilic | |
| 69480 | thermophilic | 99.999 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 5375 | microaerophile | |
| 69480 | anaerobe | 98.562 |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.964
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 5375 | whitisch steamers of the Hveragerthi area | Iceland | ISL | Europe |
| 67770 | Grayish filaments collected from a hot streamlet in Hveragerthi | Iceland | ISL | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Environmental | #Terrestrial |
Safety information
risk assessment
- @ref: 5375
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 67770
- description: Thermocrinis albus 16S rRNA gene, strain HI 11/12
- accession: AJ278895
- length: 1488
- database: ena
- NCBI tax ID: 638303
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermocrinis albus DSM 14484 | GCA_000025605 | complete | ncbi | 638303 |
| 66792 | Thermocrinis albus DSM 14484 | 638303.3 | complete | patric | 638303 |
| 66792 | Thermocrinis albus HI 11/12, DSM 14484 | 646564582 | complete | img | 638303 |
GC content
- @ref: 67770
- GC-content: 49.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 61 | no |
| motile | yes | 90.249 | no |
| flagellated | no | 86.947 | no |
| gram-positive | no | 99.429 | no |
| anaerobic | no | 54.325 | yes |
| aerobic | no | 95.081 | no |
| halophile | no | 91.94 | no |
| spore-forming | no | 96.187 | no |
| thermophile | yes | 100 | no |
| glucose-util | yes | 56.535 | no |
| glucose-ferment | no | 85.016 | no |
External links
@ref: 5375
culture collection no.: DSM 14484, JCM 11386
straininfo link
- @ref: 70202
- straininfo: 101009
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 12215816 | New isolates and physiological properties of the Aquificales and description of Thermocrinis albus sp. nov. | Eder W, Huber R | Extremophiles | 10.1007/s00792-001-0259-y | 2002 | Bacteria/classification/genetics/*isolation & purification/ultrastructure, Bacterial Physiological Phenomena, Base Sequence, DNA Primers, Microscopy, Electron, Scanning, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics | Enzymology |
| Genetics | 21304702 | Complete genome sequence of Thermocrinis albus type strain (HI 11/12). | Wirth R, Sikorski J, Brambilla E, Misra M, Lapidus A, Copeland A, Nolan M, Lucas S, Chen F, Tice H, Cheng JF, Han C, Detter JC, Tapia R, Bruce D, Goodwin L, Pitluck S, Pati A, Anderson I, Ivanova N, Mavromatis K, Mikhailova N, Chen A, Palaniappan K, Bilek Y, Hader T, Land M, Hauser L, Chang YJ, Jeffries CD, Tindall BJ, Rohde M, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP | Stand Genomic Sci | 10.4056/sigs.761490 | 2010 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 5375 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14484) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14484 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 70202 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID101009.1 | StrainInfo: A central database for resolving microbial strain identifiers |