Strain identifier
BacDive ID: 5238
Type strain:
Species: Cronobacter turicensis
Strain Designation: 3032
Strain history: <- A. Lehner; z3032 <- S. Roger, University Zürich
NCBI tax ID(s): 693216 (strain), 413502 (species)
General
@ref: 7664
BacDive-ID: 5238
DSM-Number: 18703
keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Cronobacter turicensis 3032 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from neonate with meningitidis.
NCBI tax id
NCBI tax id | Matching level |
---|---|
413502 | species |
693216 | strain |
strain history
- @ref: 7664
- history: <- A. Lehner; z3032 <- S. Roger, University Zürich
doi: 10.13145/bacdive5238.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Cronobacter
- species: Cronobacter turicensis
- full scientific name: Cronobacter turicensis Iversen et al. 2008
synonyms
- @ref: 20215
- synonym: Cronobacter turicensis
@ref: 7664
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Cronobacter
species: Cronobacter turicensis
full scientific name: Cronobacter turicensis Iversen et al. 2008
strain designation: 3032
type strain: yes
Morphology
cell morphology
- @ref: 32494
- gram stain: negative
- cell length: 3 µm
- cell width: 1 µm
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7664 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
7664 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7664 | positive | growth | 37 | mesophilic |
32494 | positive | growth | 06-45 |
culture pH
- @ref: 32494
- ability: positive
- type: growth
- pH: 05-10
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 32494
- oxygen tolerance: facultative anaerobe
halophily
- @ref: 32494
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: <7 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
32494 | 15792 | malonate | + | carbon source |
32494 | 17268 | myo-inositol | + | carbon source |
32494 | 17148 | putrescine | + | carbon source |
32494 | 4853 | esculin | + | hydrolysis |
32494 | 17632 | nitrate | + | reduction |
68368 | 30849 | L-arabinose | + | fermentation |
68368 | 27613 | amygdalin | + | fermentation |
68368 | 28053 | melibiose | + | fermentation |
68368 | 62345 | L-rhamnose | + | fermentation |
68368 | 30911 | sorbitol | - | fermentation |
68368 | 17268 | myo-inositol | + | fermentation |
68368 | 17634 | D-glucose | + | fermentation |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | + | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
32494 | catalase | + | 1.11.1.6 |
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | + | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
7664 | - | + | - | + | + | - | - | - | - | + | + | + | +/- | + | - | + | - | + | + | + | - |
7664 | + | + | - | + | + | - | - | - | - | + | +/- | + | + | + | - | + | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
7664 | neonate with meningitidis | Zürich | Switzerland | CHE | Europe | |
60757 | Human blood,neonatal,meningitis | Zürich | Switzerland | CHE | Europe | 2005 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Disease | #Meningitis |
#Infection | #Patient | |
#Host | #Human | #Child |
taxonmaps
- @ref: 69479
- File name: preview.99_317.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_241;98_270;99_317&stattab=map
- Last taxonomy: Cronobacter
- 16S sequence: EF059891
- Sequence Identity:
- Total samples: 26535
- soil counts: 1166
- aquatic counts: 2553
- animal counts: 20787
- plant counts: 2029
Safety information
risk assessment
- @ref: 7664
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7664
- description: Cronobacter turicensis z3032 16S ribosomal RNA gene, partial sequence
- accession: EF059891
- length: 1423
- database: ena
- NCBI tax ID: 693216
External links
@ref: 7664
culture collection no.: DSM 18703, LMG 23827, CCUG 55852
straininfo link
- @ref: 74729
- straininfo: 354308
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 18523192 | Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. | Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H | Int J Syst Evol Microbiol | 10.1099/ijs.0.65577-0 | 2008 | Bacterial Typing Techniques, Cronobacter sakazakii/*classification/genetics/metabolism/physiology, DNA, Bacterial/analysis, Enterobacteriaceae/*classification/genetics/metabolism/physiology, Genes, rRNA, Malonates/metabolism, Microarray Analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Reagent Kits, Diagnostic, Sequence Analysis, DNA, Species Specificity | Metabolism |
Genetics | 21037008 | Complete genome sequence of Cronobacter turicensis LMG 23827, a food-borne pathogen causing deaths in neonates. | Stephan R, Lehner A, Tischler P, Rattei T | J Bacteriol | 10.1128/JB.01162-10 | 2010 | Cronobacter sakazakii/*genetics/pathogenicity, Enterobacteriaceae Infections/*microbiology/mortality, Foodborne Diseases/*microbiology/mortality, *Genome, Bacterial, Humans, Infant, Newborn, Molecular Sequence Data | Pathogenicity |
Phylogeny | 22661070 | Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients. | Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.032292-0 | 2011 | Child, Cronobacter/*classification/genetics/*isolation & purification, DNA, Bacterial/genetics, Enterobacteriaceae Infections/*microbiology, *Food Microbiology, Humans, Leg/*microbiology, Male, Meat Products/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Slovakia, *Water Microbiology | Biotechnology |
Pathogenicity | 26060602 | Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis. | Fehr A, Eshwar AK, Neuhauss SC, Ruetten M, Lehner A, Vaughan L | Emerg Microbes Infect | 10.1038/emi.2015.29 | 2015 | Animals, Animals, Genetically Modified, Anti-Bacterial Agents/pharmacology, Cronobacter/drug effects/genetics/metabolism/*pathogenicity, *Disease Models, Animal, Disease Progression, Enterobacteriaceae Infections/immunology/*microbiology/mortality, Green Fluorescent Proteins/metabolism, Humans, Immunity, Innate, Infant, Newborn, Microscopy, Confocal, Mutation, Opportunistic Infections/immunology/*microbiology/mortality, *Zebrafish | Metabolism |
Metabolism | 29972620 | Interaction of matrix metalloproteinase-9 and Zpx in Cronobacter turicensis LMG 23827(T) mediated infections in the zebrafish model. | Eshwar AK, Wolfrum N, Stephan R, Fanning S, Lehner A | Cell Microbiol | 10.1111/cmi.12888 | 2018 | Animals, Bacterial Load, Cronobacter/genetics/metabolism/*pathogenicity, Disease Models, Animal, Embryo, Nonmammalian/microbiology, Enterobacteriaceae Infections/*metabolism, Enzyme Activation, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Gene Silencing, Host-Pathogen Interactions/*physiology, Matrix Metalloproteinase 9/genetics/*metabolism, Metalloendopeptidases/genetics/*metabolism, Recombinant Proteins/genetics/metabolism, Zebrafish/embryology | Pathogenicity |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7664 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18703) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18703 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
32494 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28714 | 28776041 | |
60757 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 55852) | https://www.ccug.se/strain?id=55852 | |||
68368 | Automatically annotated from API 20E | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
74729 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID354308.1 | StrainInfo: A central database for resolving microbial strain identifiers |