Strain identifier

BacDive ID: 5238

Type strain: Yes

Species: Cronobacter turicensis

Strain Designation: 3032

Strain history: <- A. Lehner; z3032 <- S. Roger, University Zürich

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7664

BacDive-ID: 5238

DSM-Number: 18703

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Cronobacter turicensis 3032 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from neonate with meningitidis.

NCBI tax id

NCBI tax idMatching level
413502species
693216strain

strain history

  • @ref: 7664
  • history: <- A. Lehner; z3032 <- S. Roger, University Zürich

doi: 10.13145/bacdive5238.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Cronobacter
  • species: Cronobacter turicensis
  • full scientific name: Cronobacter turicensis Iversen et al. 2008
  • synonyms

    • @ref: 20215
    • synonym: Cronobacter turicensis

@ref: 7664

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Cronobacter

species: Cronobacter turicensis

full scientific name: Cronobacter turicensis Iversen et al. 2008

strain designation: 3032

type strain: yes

Morphology

cell morphology

  • @ref: 32494
  • gram stain: negative
  • cell length: 3 µm
  • cell width: 1 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7664COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7664TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7664positivegrowth37mesophilic
32494positivegrowth06-45

culture pH

  • @ref: 32494
  • ability: positive
  • type: growth
  • pH: 05-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 32494
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 32494
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3249415792malonate+carbon source
3249417268myo-inositol+carbon source
3249417148putrescine+carbon source
324944853esculin+hydrolysis
3249417632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol+fermentation
6836817634D-glucose+fermentation
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
32494catalase+1.11.1.6
68368cytochrome oxidase-1.9.3.1
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
7664-+-++----++++/-+-+-+++-
7664++-++----++/-+++-+++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7664neonate with meningitidisZürichSwitzerlandCHEEurope
60757Human blood,neonatal,meningitisZürichSwitzerlandCHEEurope2005

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Meningitis
#Infection#Patient
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_317.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_241;98_270;99_317&stattab=map
  • Last taxonomy: Cronobacter
  • 16S sequence: EF059891
  • Sequence Identity:
  • Total samples: 26535
  • soil counts: 1166
  • aquatic counts: 2553
  • animal counts: 20787
  • plant counts: 2029

Safety information

risk assessment

  • @ref: 7664
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7664
  • description: Cronobacter turicensis z3032 16S ribosomal RNA gene, partial sequence
  • accession: EF059891
  • length: 1423
  • database: ena
  • NCBI tax ID: 693216

External links

@ref: 7664

culture collection no.: DSM 18703, LMG 23827, CCUG 55852

straininfo link

  • @ref: 74729
  • straininfo: 354308

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18523192Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten HInt J Syst Evol Microbiol10.1099/ijs.0.65577-02008Bacterial Typing Techniques, Cronobacter sakazakii/*classification/genetics/metabolism/physiology, DNA, Bacterial/analysis, Enterobacteriaceae/*classification/genetics/metabolism/physiology, Genes, rRNA, Malonates/metabolism, Microarray Analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Reagent Kits, Diagnostic, Sequence Analysis, DNA, Species SpecificityMetabolism
Genetics21037008Complete genome sequence of Cronobacter turicensis LMG 23827, a food-borne pathogen causing deaths in neonates.Stephan R, Lehner A, Tischler P, Rattei TJ Bacteriol10.1128/JB.01162-102010Cronobacter sakazakii/*genetics/pathogenicity, Enterobacteriaceae Infections/*microbiology/mortality, Foodborne Diseases/*microbiology/mortality, *Genome, Bacterial, Humans, Infant, Newborn, Molecular Sequence DataPathogenicity
Phylogeny22661070Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients.Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJInt J Syst Evol Microbiol10.1099/ijs.0.032292-02011Child, Cronobacter/*classification/genetics/*isolation & purification, DNA, Bacterial/genetics, Enterobacteriaceae Infections/*microbiology, *Food Microbiology, Humans, Leg/*microbiology, Male, Meat Products/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Slovakia, *Water MicrobiologyBiotechnology
Pathogenicity26060602Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis.Fehr A, Eshwar AK, Neuhauss SC, Ruetten M, Lehner A, Vaughan LEmerg Microbes Infect10.1038/emi.2015.292015Animals, Animals, Genetically Modified, Anti-Bacterial Agents/pharmacology, Cronobacter/drug effects/genetics/metabolism/*pathogenicity, *Disease Models, Animal, Disease Progression, Enterobacteriaceae Infections/immunology/*microbiology/mortality, Green Fluorescent Proteins/metabolism, Humans, Immunity, Innate, Infant, Newborn, Microscopy, Confocal, Mutation, Opportunistic Infections/immunology/*microbiology/mortality, *ZebrafishMetabolism
Metabolism29972620Interaction of matrix metalloproteinase-9 and Zpx in Cronobacter turicensis LMG 23827(T) mediated infections in the zebrafish model.Eshwar AK, Wolfrum N, Stephan R, Fanning S, Lehner ACell Microbiol10.1111/cmi.128882018Animals, Bacterial Load, Cronobacter/genetics/metabolism/*pathogenicity, Disease Models, Animal, Embryo, Nonmammalian/microbiology, Enterobacteriaceae Infections/*metabolism, Enzyme Activation, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Gene Silencing, Host-Pathogen Interactions/*physiology, Matrix Metalloproteinase 9/genetics/*metabolism, Metalloendopeptidases/genetics/*metabolism, Recombinant Proteins/genetics/metabolism, Zebrafish/embryologyPathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7664Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18703)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18703
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32494Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2871428776041
60757Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55852)https://www.ccug.se/strain?id=55852
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74729Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID354308.1StrainInfo: A central database for resolving microbial strain identifiers