Strain identifier

BacDive ID: 5237

Type strain: Yes

Species: Cronobacter malonaticus

Strain Designation: E825

Strain history: <- A. Lehner; E825 <- Nestec, S.A. <- CDC <- Analytabs Products Inc. Plainview, New York, USA

NCBI tax ID(s): 1159491 (strain), 413503 (species)

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General

@ref: 7663

BacDive-ID: 5237

DSM-Number: 18702

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Cronobacter malonaticus E825 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from breast abscess.

NCBI tax id

NCBI tax idMatching level
413503species
1159491strain

strain history

  • @ref: 7663
  • history: <- A. Lehner; E825 <- Nestec, S.A. <- CDC <- Analytabs Products Inc. Plainview, New York, USA

doi: 10.13145/bacdive5237.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Cronobacter
  • species: Cronobacter malonaticus
  • full scientific name: Cronobacter malonaticus Iversen et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Cronobacter sakazakii subsp. malonaticus

@ref: 7663

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Cronobacter

species: Cronobacter malonaticus

full scientific name: Cronobacter malonaticus Iversen et al. 2008

strain designation: E825

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32494negative3 µm1 µmrod-shapedyes
69480yes97.067
69480negative99.926

Culture and growth conditions

culture medium

  • @ref: 7663
  • name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/535
  • composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7663positivegrowth37mesophilic
32494positivegrowth06-45
60835positivegrowth30mesophilic

culture pH

  • @ref: 32494
  • ability: positive
  • type: growth
  • pH: 05-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32494facultative anaerobe
60835aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.048

halophily

  • @ref: 32494
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3249415792malonate+carbon source
3249417148putrescine+carbon source
324944853esculin+hydrolysis
3249417632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
32494catalase+1.11.1.6
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
7663++-++----+-++--+++++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
7663breast abscessUSAUSANorth America
60835Human breast abscessUSAUSANorth AmericaNew York,Plainview

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Abscess
#Host Body-Site#Other#Thoracic segment

taxonmaps

  • @ref: 69479
  • File name: preview.99_338.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_241;98_270;99_338&stattab=map
  • Last taxonomy: Cronobacter
  • 16S sequence: EF059881
  • Sequence Identity:
  • Total samples: 1704
  • soil counts: 36
  • aquatic counts: 140
  • animal counts: 1436
  • plant counts: 92

Safety information

risk assessment

  • @ref: 7663
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 32494
  • description: Cronobacter malonaticus strain E825 16S ribosomal RNA gene, partial sequence
  • accession: EF059881
  • length: 1417
  • database: nuccore
  • NCBI tax ID: 413503

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cronobacter malonaticus LMG 23826GCA_001277215completencbi1159491
66792Cronobacter malonaticus LMG 23826GCA_000409305contigncbi1159491
66792Cronobacter malonaticus LMG 238261159491.20plasmidpatric1159491
66792Cronobacter malonaticus LMG 238261159491.6completepatric1159491
66792Cronobacter malonaticus LMG 238261159491.19plasmidpatric1159491
66792Cronobacter malonaticus LMG 238261159491.3wgspatric1159491
66792Cronobacter malonaticus LMG 238262548876974draftimg1159491
66792Cronobacter malonaticus LMG 23826 GCA_001277215.22827186723completeimg1159491

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes92.128yes
flagellatedno77.744no
gram-positiveno97.983yes
anaerobicno96.472yes
halophileno74.811no
spore-formingno93.221no
thermophileno98.915yes
glucose-utilyes95.967no
aerobicyes78.062no
glucose-fermentyes92.151yes

External links

@ref: 7663

culture collection no.: CCUG 56035, DSM 18702, LMG 23826, API 76-2121, CDC 1058-77

straininfo link

  • @ref: 74728
  • straininfo: 354307

literature

  • topic: Phylogeny
  • Pubmed-ID: 18523192
  • title: Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.
  • authors: Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65577-0
  • year: 2008
  • mesh: Bacterial Typing Techniques, Cronobacter sakazakii/*classification/genetics/metabolism/physiology, DNA, Bacterial/analysis, Enterobacteriaceae/*classification/genetics/metabolism/physiology, Genes, rRNA, Malonates/metabolism, Microarray Analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Reagent Kits, Diagnostic, Sequence Analysis, DNA, Species Specificity
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7663Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18702)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18702
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32494Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2871428776041
60835Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56035)https://www.ccug.se/strain?id=56035
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74728Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID354307.1StrainInfo: A central database for resolving microbial strain identifiers