Strain identifier

BacDive ID: 5205

Type strain: Yes

Species: Xenorhabdus romanii

Strain Designation: PR06-A

Strain history: CIP <- 2005, N. Boemare, INRA, Montpellier Univ., Montpellier, France: strain PR06-A

NCBI tax ID(s): 351677 (species)

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General

@ref: 7241

BacDive-ID: 5205

DSM-Number: 17910

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, animal pathogen

description: Xenorhabdus romanii PR06-A is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from Steinernema puertoricense.

NCBI tax id

  • NCBI tax id: 351677
  • Matching level: species

strain history

@refhistory
7241<- P. Tailliez; PR06-A <- N. E. Boemare <- M. Fischer-Le Saux
122248CIP <- 2005, N. Boemare, INRA, Montpellier Univ., Montpellier, France: strain PR06-A

doi: 10.13145/bacdive5205.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Morganellaceae
  • genus: Xenorhabdus
  • species: Xenorhabdus romanii
  • full scientific name: Xenorhabdus romanii Tailliez et al. 2006

@ref: 7241

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Morganellaceae

genus: Xenorhabdus

species: Xenorhabdus romanii

full scientific name: Xenorhabdus romanii Tailliez et al. 2006

strain designation: PR06-A

type strain: yes

Morphology

cell morphology

  • @ref: 122248
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 122248
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7241TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
122248CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

  • @ref: 7241
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122248
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12224817632nitrate-reduction
12224816301nitrite-reduction

metabolite production

  • @ref: 122248
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122248oxidase-
122248alcohol dehydrogenase-1.1.1.1
122248catalase-1.11.1.6
122248lysine decarboxylase-4.1.1.18
122248ornithine decarboxylase-4.1.1.17
122248urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122248-+++-++---++---+-+--

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentgeographic locationisolation date
7241Steinernema puertoricenseSteinernema puertoricensePuerto RicoPRIMiddle and South America
122248Animal, Nematode Steinernema puertoricenseUnited States of AmericaUSANorth AmericaPuerto Rico1996

isolation source categories

  • Cat1: #Host
  • Cat2: #Invertebrates (Other)
  • Cat3: #Nematoda

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
7241yes1Risk group (German classification)
1222481Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7241
  • description: Xenorhabdus romanii strain PR06-A 16S ribosomal RNA gene, partial sequence
  • accession: DQ211717
  • length: 1486
  • database: ena
  • NCBI tax ID: 351677

External links

@ref: 7241

culture collection no.: DSM 17910, CIP 109070

straininfo link

  • @ref: 74697
  • straininfo: 297375

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17158981New insight into diversity in the genus Xenorhabdus, including the description of ten novel species.Tailliez P, Pages S, Ginibre N, Boemare NInt J Syst Evol Microbiol10.1099/ijs.0.64287-02006Animals, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S, Rhabditida/classification/*microbiology, Sequence Analysis, DNA, *Symbiosis, Xenorhabdus/*classification/geneticsGenetics
Phylogeny21948091Description of Xenorhabdus magdalenensis sp. nov., the symbiotic bacterium associated with Steinernema australe.Tailliez P, Pages S, Edgington S, Tymo LM, Buddie AGInt J Syst Evol Microbiol10.1099/ijs.0.034322-02011Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhabditida/*microbiology, Sequence Analysis, DNA, Symbiosis, Xenorhabdus/*classification/genetics/isolation & purificationGenetics

Reference

@idauthorscataloguedoi/urltitle
7241Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17910)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17910
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68382Automatically annotated from API zym
74697Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297375.1StrainInfo: A central database for resolving microbial strain identifiers
122248Curators of the CIPCollection of Institut Pasteur (CIP 109070)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109070