Strain identifier
BacDive ID: 5205
Type strain:
Species: Xenorhabdus romanii
Strain Designation: PR06-A
Strain history: CIP <- 2005, N. Boemare, INRA, Montpellier Univ., Montpellier, France: strain PR06-A
NCBI tax ID(s): 351677 (species)
General
@ref: 7241
BacDive-ID: 5205
DSM-Number: 17910
keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, animal pathogen
description: Xenorhabdus romanii PR06-A is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from Steinernema puertoricense.
NCBI tax id
- NCBI tax id: 351677
- Matching level: species
strain history
@ref | history |
---|---|
7241 | <- P. Tailliez; PR06-A <- N. E. Boemare <- M. Fischer-Le Saux |
122248 | CIP <- 2005, N. Boemare, INRA, Montpellier Univ., Montpellier, France: strain PR06-A |
doi: 10.13145/bacdive5205.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Morganellaceae
- genus: Xenorhabdus
- species: Xenorhabdus romanii
- full scientific name: Xenorhabdus romanii Tailliez et al. 2006
@ref: 7241
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Morganellaceae
genus: Xenorhabdus
species: Xenorhabdus romanii
full scientific name: Xenorhabdus romanii Tailliez et al. 2006
strain designation: PR06-A
type strain: yes
Morphology
cell morphology
- @ref: 122248
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 122248
- hemolysis ability: 1
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7241 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
122248 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
- @ref: 7241
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 122248
- oxygen tolerance: facultative anaerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
122248 | 17632 | nitrate | - | reduction |
122248 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 122248
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
122248 | oxidase | - | |
122248 | alcohol dehydrogenase | - | 1.1.1.1 |
122248 | catalase | - | 1.11.1.6 |
122248 | lysine decarboxylase | - | 4.1.1.18 |
122248 | ornithine decarboxylase | - | 4.1.1.17 |
122248 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122248 | - | + | + | + | - | + | + | - | - | - | + | + | - | - | - | + | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | country | origin.country | continent | geographic location | isolation date |
---|---|---|---|---|---|---|---|
7241 | Steinernema puertoricense | Steinernema puertoricense | Puerto Rico | PRI | Middle and South America | ||
122248 | Animal, Nematode Steinernema puertoricense | United States of America | USA | North America | Puerto Rico | 1996 |
isolation source categories
- Cat1: #Host
- Cat2: #Invertebrates (Other)
- Cat3: #Nematoda
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
7241 | yes | 1 | Risk group (German classification) |
122248 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 7241
- description: Xenorhabdus romanii strain PR06-A 16S ribosomal RNA gene, partial sequence
- accession: DQ211717
- length: 1486
- database: ena
- NCBI tax ID: 351677
External links
@ref: 7241
culture collection no.: DSM 17910, CIP 109070
straininfo link
- @ref: 74697
- straininfo: 297375
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17158981 | New insight into diversity in the genus Xenorhabdus, including the description of ten novel species. | Tailliez P, Pages S, Ginibre N, Boemare N | Int J Syst Evol Microbiol | 10.1099/ijs.0.64287-0 | 2006 | Animals, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S, Rhabditida/classification/*microbiology, Sequence Analysis, DNA, *Symbiosis, Xenorhabdus/*classification/genetics | Genetics |
Phylogeny | 21948091 | Description of Xenorhabdus magdalenensis sp. nov., the symbiotic bacterium associated with Steinernema australe. | Tailliez P, Pages S, Edgington S, Tymo LM, Buddie AG | Int J Syst Evol Microbiol | 10.1099/ijs.0.034322-0 | 2011 | Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhabditida/*microbiology, Sequence Analysis, DNA, Symbiosis, Xenorhabdus/*classification/genetics/isolation & purification | Genetics |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
7241 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17910) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17910 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
68382 | Automatically annotated from API zym | |||
74697 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297375.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
122248 | Curators of the CIP | Collection of Institut Pasteur (CIP 109070) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109070 |