Strain identifier

BacDive ID: 5164

Type strain: Yes

Species: Serratia entomophila

Strain Designation: A1

Strain history: CIP <- 1987, P.A.D. Grimont, Inst. Pasteur, Paris, France <- T.A. Jackson: strain A1

NCBI tax ID(s): 42906 (species)

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General

@ref: 4619

BacDive-ID: 5164

DSM-Number: 12358

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, oval-shaped, animal pathogen

description: Serratia entomophila A1 is an aerobe, mesophilic, Gram-negative animal pathogen that was isolated from diseased grass grub larva .

NCBI tax id

  • NCBI tax id: 42906
  • Matching level: species

strain history

@refhistory
4619<- CIP <- P.A.D. Grimont <- T.A. Jackson, A1
119087CIP <- 1987, P.A.D. Grimont, Inst. Pasteur, Paris, France <- T.A. Jackson: strain A1

doi: 10.13145/bacdive5164.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Yersiniaceae
  • genus: Serratia
  • species: Serratia entomophila
  • full scientific name: Serratia entomophila Grimont et al. 1988

@ref: 4619

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Yersiniaceae

genus: Serratia

species: Serratia entomophila

full scientific name: Serratia entomophila Grimont et al. 1988

strain designation: A1

type strain: yes

Morphology

cell morphology

  • @ref: 119087
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: yes

colony morphology

  • @ref: 60614
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4619CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
33752MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119087CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
119087CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4619positivegrowth28mesophilic
33752positivegrowth30mesophilic
60614positivegrowth30-37mesophilic
119087positivegrowth5-37
119087nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
60614aerobe
119087facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate+builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371L-fucose+builds acid from18287
68371D-tagatose-builds acid from16443
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371esculin+builds acid from4853
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371L-xylose-builds acid from65328
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
68368nitrate+reduction17632
68368L-arabinose-fermentation30849
68368amygdalin+fermentation27613
68368melibiose+fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol+fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin+hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate+assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
119087mannitol+fermentation29864
119087citrate+carbon source16947
119087glucose+fermentation17234
119087hippurate-hydrolysis606565
119087lactose-fermentation17716
119087nitrate+reduction17632
119087nitrite-reduction16301
119087malonate-assimilation15792
119087sodium thiosulfate-builds gas from132112

metabolite production

@refChebi-IDmetaboliteproduction
6836817997dinitrogenno
6836816301nitriteyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11908735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11908715688acetoin+
11908717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
119087oxidase-
119087beta-galactosidase+3.2.1.23
119087alcohol dehydrogenase-1.1.1.1
119087gelatinase+
119087catalase+1.11.1.6
119087gamma-glutamyltransferase+2.3.2.2
119087lysine decarboxylase-4.1.1.18
119087ornithine decarboxylase-4.1.1.17
119087phenylalanine ammonia-lyase-4.3.1.24
119087tryptophan deaminase-
119087urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119087-++++++++-++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOXNO2N2
4619+---+----+++++--+++--+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119087+/--+/-+++/--+/--+/-++/-+/----+/-+/----+/--+/-++/-+/-+/-+/-+/-++/----+/---+/--+/--+/-++/---++/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119087+++++--+-++----+++++----+-+----+++---+-+---------+++-+++++++++--+--+----++-++++++-----++-++++++---+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
4619diseased grass grub larva (Costelytra zealandica)Costelytra zealandicaFairtonNew ZealandNZLAustralia and Oceania
60614Diseased grass grub larva,Costelytra zealandicaFairtonNew ZealandNZLAustralia and Oceania
119087Environment, Diseased grass grub larva, Costelytra zealandicaFairtonNew ZealandNZLAustralia and Oceania

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
4619yes1Risk group (German classification)
1190871Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Serratia entomophila 16S rRNA gene (strain DSM 12358)
  • accession: AJ233427
  • length: 1516
  • database: ena
  • NCBI tax ID: 42906

Genome sequences

  • @ref: 66792
  • description: Serratia entomophila A1
  • accession: GCA_021462285
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 42906

GC content

  • @ref: 4619
  • GC-content: 58

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno60.499no
gram-positiveno97.833no
anaerobicno98.605yes
aerobicyes89.554no
halophileno93.305no
spore-formingno95.145no
motileyes90.212no
glucose-fermentyes90.619no
thermophileno99.525yes
glucose-utilyes95.5no

External links

@ref: 4619

culture collection no.: CCUG 55496, LMG 8456, KCTC 2934, NCIMB 12800, BCRC 15867, DSM 12358, ATCC 43705, CIP 102919

straininfo link

  • @ref: 74656
  • straininfo: 10849

literature

  • topic: Phylogeny
  • Pubmed-ID: 31089912
  • title: Serratia microhaemolytica sp. nov., isolated from an artificial lake in Southern China.
  • authors: Wang J, Zheng ML, Jiao JY, Wang WJ, Li S, Xiao M, Chen C, Qu PH, Li WJ
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-019-01273-9
  • year: 2019
  • mesh: Anaerobiosis, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fresh Water/microbiology, Hydrogen-Ion Concentration, Lakes/*microbiology, Locomotion, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Serratia/*classification/genetics/*isolation & purification/physiology, Sodium Chloride/metabolism, Temperature
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4619Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12358)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12358
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33752Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14659
60614Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55496)https://www.ccug.se/strain?id=55496
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
74656Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10849.1StrainInfo: A central database for resolving microbial strain identifiers
119087Curators of the CIPCollection of Institut Pasteur (CIP 102919)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102919