Strain identifier
BacDive ID: 515
Type strain: 
Species: Persephonella marina
Strain Designation: Ex-H1T
Strain history: <- D. Götz; Ex-H1T <- A.-L. Reysenbach
NCBI tax ID(s): 309805 (species)
version 8.1 (current version)
General
@ref: 5316
BacDive-ID: 515
DSM-Number: 14350
keywords: 16S sequence, Bacteria, chemolithoautotroph, thermophilic, Gram-negative, motile, rod-shaped
description: Persephonella marina Ex-H1T is a chemolithoautotroph, thermophilic, Gram-negative bacterium that was isolated from deep-sea hydrothermal vent.
NCBI tax id
- NCBI tax id: 309805
- Matching level: species
strain history
- @ref: 5316
- history: <- D. Götz; Ex-H1T <- A.-L. Reysenbach
doi: 10.13145/bacdive515.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/aquificota
- domain: Bacteria
- phylum: Aquificota
- class: Aquificia
- order: Aquificales
- family: Hydrogenothermaceae
- genus: Persephonella
- species: Persephonella marina
- full scientific name: Persephonella marina Götz et al. 2002
@ref: 5316
domain: Bacteria
phylum: Aquificae
class: Aquificae
order: Aquificales
family: Hydrogenothermaceae
genus: Persephonella
species: Persephonella marina
full scientific name: Persephonella marina Götz et al. 2002
strain designation: Ex-H1T
type strain: yes
Morphology
cell morphology
- @ref: 23129
- gram stain: negative
- cell length: 2-4 µm
- cell width: 0.3-0.4 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 23129
- colony color: apricot-pink
Culture and growth conditions
culture medium
- @ref: 5316
- name: PERSEPHONELLA MEDIUM (DSMZ Medium 996)
- growth: yes
- link: https://mediadive.dsmz.de/medium/996
- composition: Name: PERSEPHONELLA MEDIUM (DSMZ Medium 996) Composition: NaCl 29.0 g/l MgSO4 x 7 H2O 7.0 g/l Na2S2O3 x 5 H2O 2.0 g/l NaOH 2.0 g/l MgCl2 x 6 H2O 1.36 g/l KCl 0.5 g/l CaCl2 x 2 H2O 0.4 g/l K2HPO4 x 3 H2O 0.3 g/l NH4Cl 0.2 g/l Na-EDTA x 2 H2O 0.005 g/l CoCl2 x 6 H2O 0.0015 g/l ZnCl2 0.001 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l AlCl3 x 6 H2O 0.0004 g/l Na2O4W x 2 H2O 0.0003 g/l CuCl 0.0002 g/l NiSO4 x 6 H2O 0.0002 g/l H2SeO3 0.0001 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 5316 | positive | growth | 70 | thermophilic |
| 23129 | positive | maximum | 80 | hyperthermophilic |
| 23129 | positive | minimum | 55 | thermophilic |
| 23129 | positive | optimum | 73 | thermophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 23129 | positive | growth | 4.7-7.5 |
| 23129 | positive | optimum | 6.0 |
Physiology and metabolism
nutrition type
- @ref: 23129
- type: chemolithoautotroph
spore formation
- @ref: 23129
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 23129 | NaCl | positive | growth | 1.0-4.5 %(w/v) |
| 23129 | NaCl | positive | optimum | 2.5 %(w/v) |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 23129 | 17632 | nitrate | + | electron acceptor |
| 23129 | 33403 | elemental sulfur | + | electron donor |
| 23129 | 15740 | formate | + | electron donor |
| 23129 | 16094 | thiosulfate | + | electron donor |
Isolation, sampling and environmental information
isolation
- @ref: 5316
- sample type: deep-sea hydrothermal vent
- geographic location: East Pacific Rise, 9°N 104°W
- country: Pacific Ocean
- latitude: 9
- longitude: -104
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Environmental | #Aquatic | #Marine |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_6170.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15894;96_2939;97_3614;98_4574;99_6170&stattab=map
- Last taxonomy: Persephonella marina subclade
- 16S sequence: AF188332
- Sequence Identity:
- Total samples: 60
- soil counts: 5
- aquatic counts: 17
- animal counts: 38
Safety information
risk assessment
- @ref: 5316
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Persephonella marina 16S-23S rRNA intergenic spacer containing tRNA-Ile and tRNA-Ala genes, type strain DSM 14350T | AM259506 | 368 | ena | 123214 |
| 5316 | Persephonella marina 16S ribosomal RNA gene, partial sequence | AF188332 | 1463 | ena | 123214 |
GC content
- @ref: 5316
- GC-content: 52
External links
@ref: 5316
culture collection no.: DSM 14350, OCM 794
straininfo link
- @ref: 70194
- straininfo: 100860
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 5316 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14350) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14350 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 23129 | D Götz, A Banta, T J Beveridge, A I Rushdi, B R T Simoneit, A L Reysenbach | 10.1099/00207713-52-4-1349 | Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents. | IJSEM 52: 1349-1359 2002 | 12148650 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 70194 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100860.1 | StrainInfo: A central database for resolving microbial strain identifiers |