Strain identifier

BacDive ID: 5117

Type strain: Yes

Species: Salmonella enterica

Strain Designation: LT2

Culture col. no.: DSM 17058, ATCC 43971, CIP 60.62, NCIMB 11450, NCTC 12416, CCUG 42060

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6727

BacDive-ID: 5117

DSM-Number: 17058

keywords: aerobe, Bacteria, 16S sequence, genome sequence, mesophilic

description: Salmonella enterica LT2 is an aerobe, mesophilic bacterium of the family Enterobacteriaceae.

strain history: <- CIP; CIP 60.62 <- M. Demerec, Carnegie Institute, Cold Spring Harbor, NY; LT2

doi: 10.13145/bacdive5117.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Salmonella
  • species: Salmonella enterica
  • full scientific name: Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987
  • synonyms

    @refsynonym
    20215Bacillus typhimurium
    20215Bacillus typhi
    20215Salmonella choleraesuis
    20215Salmonella typhimurium
    20215Bacillus enteritidis
    20215Bacillus choleraesuis
    20215Salmonella paratyphi
    20215Salmonella enteritidis
    20215Salmonella typhi

@ref: 6727

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Salmonella

species: Salmonella enterica

full scientific name: Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987

strain designation: LT2

type strain: yes

Morphology

cell morphology

  • @ref: 22945
  • cell length: 2 µm
  • cell width: 0.5-1.5 µm

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6727COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
6727TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf
33584MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
6727positivegrowth37mesophilic
33584positivegrowth30mesophilic
55324positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 55324
  • oxygen tolerance: aerobe

compound production

  • @ref: 22945
  • compound: Hydrogen sulfide

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
22945168082-dehydro-D-gluconate-growth
22945479273-coumarate-growth
22945739183-O-methyl-D-glucose-growth
22945510573-phenylpropionate-growth
22945863945-aminovalerate-growth
2294515963ribitol-builds acid from
2294515963ribitol-growth
2294522599arabinose+builds acid from
2294516150benzoate-growth
2294517750betaine-growth
2294527689decanoate-growth
2294525646octanoate-growth
2294517057cellobiose-builds acid from
2294516383cis-aconitate+growth
2294516947citrate+growth
2294515570D-alanine+growth
2294518333D-arabitol-growth
2294517057cellobiose-growth
2294515824D-fructose+growth
2294512936D-galactose+growth
2294518024D-galacturonic acid-growth
2294530612D-glucarate+growth
229458391D-gluconate+growth
2294517315D-glucosamine+growth
2294517634D-glucose+growth
2294515748D-glucuronate+growth
2294562318D-lyxose-growth
2294516899D-mannitol+growth
2294516024D-mannose+growth
2294528053melibiose+growth
2294516634raffinose-growth
2294516988D-ribose+growth
2294517924D-sorbitol+growth
2294516443D-tagatose+growth
2294516551D-trehalose+growth
2294565327D-xylose+growth
22945370543-hydroxybutyrate-growth
2294533871glycerate+growth
2294524996lactate+growth
2294517113erythritol-growth
229454853esculin-growth
2294516000ethanolamine+growth
2294529806fumarate+growth
2294516537galactarate+builds acid from
2294516537galactarate+growth
2294516813galactitol+builds acid from
2294516813galactitol+growth
2294524175galacturonate-builds acid from
229455291gelatin-hydrolysis
2294528066gentiobiose-growth
2294558044gentisate+growth
2294517234glucose+builds gas from
2294517859glutarate-growth
2294517754glycerol+growth
2294518295histamine-growth
2294517268myo-inositol+builds acid from
2294516977L-alanine+growth
2294530849L-arabinose+growth
2294518403L-arabitol-growth
2294529991L-aspartate+growth
2294518287L-fucose+growth
2294529985L-glutamate+growth
2294515589L-malate+growth
2294517203L-proline+growth
2294562345L-rhamnose+growth
2294517115L-serine+growth
2294517266L-sorbose-growth
2294530924L-tartrate+growth
2294517716lactose-builds acid from
2294517716lactose-growth
229456359lactulose-growth
2294515792malonate-growth
2294568428maltitol-growth
2294517306maltose+builds acid from
2294517306maltose+growth
2294561993maltotriose+growth
2294529864mannitol+builds acid from
2294537684mannose+builds acid from
229456731melezitose-growth
2294528053melibiose+builds acid from
2294530928meso-tartrate+growth
2294555507methyl alpha-D-galactoside+growth
22945320061methyl alpha-D-glucopyranoside+growth
2294517540methyl beta-D-galactoside-growth
2294517268myo-inositol+growth
22945506227N-acetyl-D-glucosamine+growth
22945309162-oxoglutarate-growth
2294518394palatinose-growth
2294518401phenylacetate-growth
2294517272propionate+growth
2294536241protocatechuate-growth
2294517148putrescine-growth
2294526490quinate-growth
2294516634raffinose-builds acid from
2294526546rhamnose+builds acid from
2294517814salicin-builds acid from
2294530911sorbitol+builds acid from
2294530031succinate+growth
2294517992sucrose-builds acid from
2294517992sucrose-growth
2294515708trans-aconitate-growth
2294527082trehalose+builds acid from
2294562517tricarballylate+growth
2294518123trigonelline-growth
2294516765tryptamine-growth
2294532528turanose-growth
2294516199urea-hydrolysis
2294517151xylitol+builds acid from
2294517151xylitol-growth
2294516865gamma-aminobutyric acid-growth
2294517632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
2294535581indoleno
2294516136hydrogen sulfideyes

enzymes

@refvalueactivityec
22945beta-galactosidase-3.2.1.23
22945beta-glucuronidase-3.2.1.31
22945catalase+1.11.1.6
22945gelatinase-
22945lysine decarboxylase+4.1.1.18
22945ornithine decarboxylase+4.1.1.17
22945cytochrome oxidase-1.9.3.1
22945urease-3.5.1.5

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
6727-+++++---+-+++++-+-+-
6727-+++++-----+++++-+-+-
6727-+++++---+-+++++-+-+-
6727-+++++---+/--+++++-+-+-

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
55324+++---+++-++-------+-+--+-++-+--

Safety information

risk assessment

  • @ref: 6727
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Salmonella enterica subsp. enterica strain DSM 17058 16S ribosomal RNA gene, partial sequenceHQ012017936ena59201
20218Salmonella enterica strain LT2 operon rrn, 16S-23S intergenic spacer region and tRNA-Glu gene, complete sequenceAF046813393ena28901
20218Salmonella enterica strain LT2 operon rrn, 16S-23S intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequencesAF046814516ena28901
20218Salmonella enterica subsp. enterica serovar Typhimurium gene for 16S rRNA, partial sequenceD12814326ena90371
20218Salmonella enterica subsp. enterica gene for 16S rRNA, partial sequence, strain: NBRC 13245AB6803801467ena59201
6727Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, complete genomeAE0064684857450ena99287

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Salmonella enterica subsp. enterica NCTC12416GCA_900456445contigncbi59201
66792Salmonella enterica subsp. enterica strain NCTC1241659201.937wgspatric59201

External links

@ref: 6727

culture collection no.: DSM 17058, ATCC 43971, CIP 60.62, NCIMB 11450, NCTC 12416, CCUG 42060

straininfo link

@refpassport
20218http://www.straininfo.net/strains/382808
20218http://www.straininfo.net/strains/43351
20218http://www.straininfo.net/strains/229656
20218http://www.straininfo.net/strains/43355
20218http://www.straininfo.net/strains/210058

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6727Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17058)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17058
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22945Leon Le Minor, Michel Y. Popoff10.1099/00207713-37-4-465Designation of Salmonella enterica sp. nov., nom. rev., as the Type and Only Species of the Genus Salmonella: Request for an OpinionIJSEM 37: 465-468 198710319519
33584Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9880
55324Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42060)https://www.ccug.se/strain?id=42060
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)