Strain identifier
BacDive ID: 508
Type strain: 
Species: Aquifex pyrophilus
Strain Designation: Kol5a
Strain history: DSM 6858 <-- K. O. Stetter Kol5a.
NCBI tax ID(s): 2714 (species)
version 8.1 (current version)
General
@ref: 2861
BacDive-ID: 508
DSM-Number: 6858
keywords: 16S sequence, Bacteria, hyperthermophilic
description: Aquifex pyrophilus Kol5a is a hyperthermophilic bacterium that was isolated from hot marine sediments.
NCBI tax id
- NCBI tax id: 2714
- Matching level: species
strain history
| @ref | history |
|---|---|
| 2861 | <- K.O. Stetter, Kol5a |
| 67770 | DSM 6858 <-- K. O. Stetter Kol5a. |
doi: 10.13145/bacdive508.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/aquificota
- domain: Bacteria
- phylum: Aquificota
- class: Aquificia
- order: Aquificales
- family: Aquificaceae
- genus: Aquifex
- species: Aquifex pyrophilus
- full scientific name: Aquifex pyrophilus Huber and Stetter 1992
@ref: 2861
domain: Bacteria
phylum: Aquificae
class: Aquificae
order: Aquificales
family: Aquificaceae
genus: Aquifex
species: Aquifex pyrophilus
full scientific name: Aquifex pyrophilus Huber and Stetter 1992
strain designation: Kol5a
type strain: yes
Culture and growth conditions
culture medium
- @ref: 2861
- name: MODIFIED SME MEDIUM (DSMZ Medium 534)
- growth: yes
- link: https://mediadive.dsmz.de/medium/534
- composition: Name: MODIFIED SME MEDIUM (DSMZ Medium 534) Composition: MgCl2 x 6 H2O 5.5 g/l Na2S2O3 x 5 H2O 2.0 g/l NaHCO3 2.0 g/l KCl 0.65 g/l NH4Cl 0.15 g/l K2HPO4 0.15 g/l NaBr 0.1 g/l MgSO4 x 7 H2O 0.03 g/l (NH4)2Ni(SO4)2 0.02 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Na2WO4 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2SeO4 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 2861 | positive | growth | 85 | hyperthermophilic |
| 67770 | positive | growth | 85 | hyperthermophilic |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 2861 | hot marine sediments | Kolbeinsey Ridge north of Iceland | Iceland | ISL | Europe |
| 67770 | Submarine hot sandy sediment at a depth of 106 m at the Kolbeinsey Ridge north of Iceland |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_75205.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16076;96_720;97_843;98_52593;99_75205&stattab=map
- Last taxonomy: Aquifex pyrophilus subclade
- 16S sequence: M83548
- Sequence Identity:
- Total samples: 270
- aquatic counts: 270
Safety information
risk assessment
- @ref: 2861
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Aquifex pyrophilus 16S-23S rRNA intergenic spacer containing tRNA-Ile and tRNA-Ala genes, type strain DSM 6858T | AM259526 | 366 | ena | 2714 |
| 2861 | Aquifex pyrophilus 16S ribosomal RNA gene, partial sequence | M83548 | 1564 | ena | 2714 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 67770 | 39 | thermal denaturation, midpoint method (Tm) |
| 67770 | 41.5 |
External links
@ref: 2861
culture collection no.: DSM 6858, JCM 9492
straininfo link
- @ref: 70187
- straininfo: 48306
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2861 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6858) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6858 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 70187 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48306.1 | StrainInfo: A central database for resolving microbial strain identifiers |