Strain identifier

BacDive ID: 5058

Type strain: Yes

Species: Proteus mirabilis

Strain history: R. Sakazaki 4030 <-- ATCC 29906 <-- CDC PR14.

NCBI tax ID(s): 525369 (strain), 584 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1692

BacDive-ID: 5058

DSM-Number: 4479

keywords: human pathogen, antibiotic resistance, mesophilic, Bacteria, 16S sequence, genome sequence

description: Proteus mirabilis DSM 4479 is a mesophilic human pathogen that has multiple antibiotic resistances.

NCBI tax id

NCBI tax idMatching level
584species
525369strain

strain history

doi: 10.13145/bacdive5058.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Morganellaceae
  • genus: Proteus
  • species: Proteus mirabilis
  • full scientific name: Proteus mirabilis Hauser 1885 (Approved Lists 1980)

@ref: 1692

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Morganellaceae

genus: Proteus

species: Proteus mirabilis

full scientific name: Proteus mirabilis Hauser 1885

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1692COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1692NUTRIENT AGAR (DSMZ Medium 1)yeshttps://bacmedia.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37129MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
1692TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://bacmedia.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
1692positivegrowth37mesophilic
37129positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
1692Mueller-Hinton Agar371aerob2603036-383230264442-4432122020-222200036-3840008002220-22642036-383210-12001034
1692Mueller-Hinton Agar371aerob22028-30383028-3034-364242-44328222222-2400038400268002420-22642638-4032800030-32
1692Mueller-Hinton Agar22028-3038323028444632-34820-2222-242400036-38400261000342004604032000034
1692Mueller Hinton30aerob2002838323026-28464834624222400040420281006262064603430-326100034

compound production

  • @ref: 1692
  • compound: volatile organic compounds

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836816136hydrogen sulfideyes

metabolite tests

  • @ref: 68368
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
1692---++++++/--++---------
1692---+++++--++----+/-----

Safety information

risk assessment

  • @ref: 1692
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Proteus mirabilis strain ATCC 29906 16S ribosomal RNA gene, partial sequenceAF0085821497ena584
20218Proteus mirabilis 16S rRNA gene, strain CIP103181TAJ3016821503ena584
20218Proteus vulgaris 16S rRNA gene, strain CIP103181TAJ3016831505ena585
20218Proteus mirabilis gene for 16S rRNA, partial sequence, strain: JCM 1669AB6261231465ena584
1692Proteus mirabilis strain NCTC 11938 16S ribosomal RNA gene, partial sequenceDQ8852561503ena584
67770Proteus mirabilis gene for 16S ribosomal RNA, partial sequence, strain: JCM 1669LC0609121484ena584

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Proteus mirabilis ATCC 29906 ATCC 29906GCA_000160755scaffoldncbi525369
66792Proteus mirabilis NCTC11938GCA_900455245contigncbi584
66792Proteus mirabilis ATCC 29906525369.4wgspatric525369
66792Proteus mirabilis strain NCTC11938584.639wgspatric584
66792Proteus mirabilis ATCC 29906643886024draftimg525369
67770Proteus mirabilis ATCC 29906, whole genome shotgun sequencing projectACLE00000000ncbi525369

External links

@ref: 1692

culture collection no.: DSM 4479, ATCC 29906, CIP 103181, NCTC 11938, WDCM 00023, CDC PR 14, JCM 1669, CCUG 26767, CECT 4168, KCTC 2566, LMG 3257, NBRC 105697

straininfo link

@refpassport
20218http://www.straininfo.net/strains/153957
20218http://www.straininfo.net/strains/16409
20218http://www.straininfo.net/strains/866304

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism8522463Imipenem resistance in clinical isolates of Proteus mirabilis associated with alterations in penicillin-binding proteins.Neuwirth C, Siebor E, Duez JM, Pechinot A, Kazmierczak AJ Antimicrob Chemother10.1093/jac/36.2.3351995Amdinocillin/pharmacology, Bacterial Outer Membrane Proteins/metabolism, *Bacterial Proteins, Carrier Proteins/*metabolism, Drug Resistance, Microbial, Electrophoresis, Polyacrylamide Gel, *Hexosyltransferases, Humans, Imipenem/*pharmacology, Isoelectric Focusing, Muramoylpentapeptide Carboxypeptidase/*metabolism, Penicillin Resistance, Penicillin-Binding Proteins, Penicillins/pharmacology, *Peptidyl Transferases, Proteus Infections/microbiology, Proteus mirabilis/*drug effects/enzymology/metabolism, Thienamycins/*pharmacology, beta-Lactamases/metabolismEnzymology
Genetics12121936DNA gyrase and topoisomerase IV mutations associated with fluoroquinolone resistance in Proteus mirabilis.Weigel LM, Anderson GJ, Tenover FCAntimicrob Agents Chemother10.1128/AAC.46.8.2582-2587.20022002Amino Acid Sequence, Amino Acid Substitution/genetics, Anti-Infective Agents/*pharmacology, DNA Gyrase/*genetics, DNA Primers, DNA Topoisomerase IV/*genetics, DNA, Bacterial/analysis/genetics, Drug Resistance, Microbial, Fluoroquinolones, Microbial Sensitivity Tests, Molecular Sequence Data, Mutation/genetics, Proteus mirabilis/*genetics, Reverse Transcriptase Polymerase Chain Reaction
Enzymology12499163Integration of a transposon Tn1-encoded inhibitor-resistant beta-lactamase gene, bla(TEM-67) from Proteus mirabilis, into the Escherichia coli chromosome.Naas T, Zerbib M, Girlich D, Nordmann PAntimicrob Agents Chemother10.1128/AAC.47.1.19-26.20032003Aged, Aged, 80 and over, Amino Acid Sequence, DNA Transposable Elements, Drug Resistance, Bacterial/*genetics, Electrophoresis, Gel, Pulsed-Field, Escherichia coli/*genetics, Female, Humans, Plasmids, Proteus mirabilis/drug effects/*genetics/isolation & purification, beta-Lactamases/*geneticsPathogenicity
Metabolism12871724Enhanced microbial biomass assay using mutant luciferase resistant to benzalkonium chloride.Hattori N, Sakakibara T, Kajiyama N, Igarashi T, Maeda M, Murakami SAnal Biochem10.1016/s0003-2697(03)00322-12003Adenosine Triphosphate/*analysis/metabolism, Benzalkonium Compounds/*chemistry/pharmacology, *Biomass, Colony Count, Microbial, Drug Resistance, Bacterial, Gram-Negative Bacteria/growth & development/metabolism, Gram-Positive Bacteria/growth & development/metabolism, Kinetics, Luciferases/*analysis/genetics/metabolism, Luminescent Measurements, Trichloroacetic Acid/chemistryEnzymology
Genetics20566768Chromosomally encoded blaCMY-2 located on a novel SXT/R391-related integrating conjugative element in a Proteus mirabilis clinical isolate.Harada S, Ishii Y, Saga T, Tateda K, Yamaguchi KAntimicrob Agents Chemother10.1128/AAC.00111-102010DNA Transposable Elements/*genetics, DNA, Bacterial/genetics, Escherichia coli/genetics, Klebsiella pneumoniae/genetics, Molecular Sequence Data, Polymerase Chain Reaction, Proteus mirabilis/*genetics, Salmonella typhimurium/genetics, beta-Lactamases/*genetics
Enzymology21298676Expression and characterization of a second L-amino acid deaminase isolated from Proteus mirabilis in Escherichia coli.Baek JO, Seo JW, Kwon O, Seong SI, Kim IH, Kim CHJ Basic Microbiol10.1002/jobm.2010000862011Amino Acid Sequence, Ammonia-Lyases/biosynthesis/genetics/isolation & purification/*metabolism, Base Sequence, Cloning, Molecular, DNA, Bacterial/chemistry/genetics, Escherichia coli/enzymology/genetics, Kinetics, Molecular Sequence Data, Polymerase Chain Reaction, Proteus mirabilis/*enzymology/genetics, Recombinant Proteins/biosynthesis/chemistry/genetics, Sequence Alignment, Substrate SpecificityGenetics
Pathogenicity23856977Convenient syntheses of cyanuric chloride-derived NHC ligands, their Ag(I) and Au(I) complexes and antimicrobial activity.Almalioti F, MacDougall J, Hughes S, Hasson MM, Jenkins RL, Ward BD, Tizzard GJ, Coles SJ, Williams DW, Bamford S, Fallis IA, Dervisi ADalton Trans10.1039/c3dt51400e2013Anti-Infective Agents/*chemical synthesis/chemistry/pharmacology, Bacteria/drug effects, Candida albicans/drug effects, Coordination Complexes/*chemical synthesis/chemistry/pharmacology, Gold/*chemistry, Heterocyclic Compounds/chemistry, Imidazoles/chemistry, Ligands, Methane/*analogs & derivatives/chemistry, Silver/*chemistry, Triazines/*chemistry
Metabolism26109004Production of phenylpyruvic acid from L-phenylalanine using an L-amino acid deaminase from Proteus mirabilis: comparison of enzymatic and whole-cell biotransformation approaches.Hou Y, Hossain GS, Li J, Shin HD, Liu L, Du GAppl Microbiol Biotechnol10.1007/s00253-015-6757-02015Amino Acids/*metabolism, Ammonia-Lyases/genetics/*metabolism, Biotransformation, Cloning, Molecular, Escherichia coli/genetics/*metabolism, Gene Expression, Phenylalanine/*metabolism, Phenylpyruvic Acids/*metabolism, Proteus mirabilis/*enzymology/genetics, Recombinant Proteins/genetics/isolation & purification/metabolismEnzymology
Metabolism26552798Combination of phenylpyruvic acid (PPA) pathway engineering and molecular engineering of L-amino acid deaminase improves PPA production with an Escherichia coli whole-cell biocatalyst.Hou Y, Hossain GS, Li J, Shin HD, Du G, Liu LAppl Microbiol Biotechnol10.1007/s00253-015-7048-52015Amidohydrolases/genetics/*metabolism, Biotransformation, Escherichia coli/*enzymology/genetics/*metabolism, Gene Knockout Techniques, *Metabolic Engineering, Metabolic Networks and Pathways/*genetics, Mutant Proteins/genetics/metabolism, Phenylalanine/metabolism, Phenylpyruvic Acids/*metabolism, Protein Engineering, Proteus mirabilis/enzymology/genetics, Recombinant Proteins/genetics/metabolism, Recombination, GeneticBiotechnology
Enzymology26747095qnrA6 genetic environment and quinolone resistance conferred on Proteus mirabilis.Jayol A, Janvier F, Guillard T, Chau F, Merens A, Robert J, Fantin B, Bercot B, Cambau EJ Antimicrob Chemother10.1093/jac/dkv4312016Anti-Bacterial Agents/*pharmacology, Bacterial Proteins/genetics/*metabolism, Chromosomes, Bacterial/genetics, Cloning, Molecular, Drug Resistance, Bacterial/*genetics, Escherichia coli/drug effects/genetics, Microbial Sensitivity Tests, Plasmids/genetics, Promoter Regions, Genetic/genetics, Proteus mirabilis/*drug effects/enzymology/*genetics, Quinolones/*pharmacologyPathogenicity
Phylogeny29509133Proteus alimentorum sp. nov., isolated from pork and lobster in Ma'anshan city, China.Dai H, Wang Y, Fang Y, Huang Z, Kan B, Wang DInt J Syst Evol Microbiol10.1099/ijsem.0.0026892018Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nephropidae, Nucleic Acid Hybridization, *Phylogeny, Proteus/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Red Meat/*microbiology, Seafood/*microbiology, Sequence Analysis, DNA, SwineTranscriptome
Pathogenicity29726711The impact of ethanol extract of propolis on biofilm forming by Proteus Mirabilis strains isolated from chronic wounds infections.Kwiecinska-Pirog J, Skowron K, Sniegowska A, Przekwas J, Balcerek M, Zaluski D, Gospodarek-Komkowska ENat Prod Res10.1080/14786419.2018.14705132018Anti-Bacterial Agents/isolation & purification/*pharmacology, Biofilms/*drug effects, Ethanol/chemistry, Humans, Propolis/*pharmacology, Proteus Infections/*drug therapy, Proteus mirabilis/*ultrastructure, Wound Healing/drug effects, Wounds and Injuries/*microbiologyEnzymology
Pathogenicity31104302Analysis of inflammatory cytokine expression in the urinary tract of BALB/c mice infected with Proteus (P.) mirabilis and enteroaggregative Escherichia (E.) coli (EAEC) strains.Melendez-Avalos A, Sainz-Espunes T, Castrillon-Rivera LE, Mendoza-Perez F, Palma-Ramos A, Castaneda-Sanchez JI, Drago-Serrano EMFolia Microbiol (Praha)10.1007/s12223-019-00714-22019Animals, Cytokines/*genetics/immunology, Escherichia coli/pathogenicity, Escherichia coli Infections/*immunology/urine, Female, Inflammation/*immunology/microbiology, Kidney/microbiology, Mice, Mice, Inbred BALB C, Proteus Infections/*immunology/urine, Proteus mirabilis/pathogenicity, Urinary Bladder/microbiology, Urinary Tract/immunology/microbiology, Urinary Tract Infections/*immunology/microbiology
31921012Characterization of a Novel Diarrheagenic Strain of Proteus mirabilis Associated With Food Poisoning in China.Gong Z, Shi X, Bai F, He X, Zhang H, Li Y, Wan Y, Lin Y, Qiu Y, Chen Q, Hu Q, Cao HFront Microbiol10.3389/fmicb.2019.028102019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1692Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4479)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4479
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37129Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14950
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E