Strain identifier

BacDive ID: 5055

Type strain: Yes

Species: Plesiomonas shigelloides

Strain Designation: M51, Bader M51

Strain history: CIP <- 1963, NCIB <- 1961, W.H. Ewing: strain Bader M51

NCBI tax ID(s): 1354269 (strain), 703 (species)

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General

@ref: 3211

BacDive-ID: 5055

DSM-Number: 8224

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, toxin production, human pathogen

description: Plesiomonas shigelloides M51 is an aerobe, mesophilic, Gram-negative human pathogen that produces toxins and was isolated from dog faeces.

NCBI tax id

NCBI tax idMatching level
1354269strain
703species

strain history

@refhistory
3211<- ATCC <- CDC, 3085-55 (Aeromonas shigelloides)
122586CIP <- 1963, NCIB <- 1961, W.H. Ewing: strain Bader M51

doi: 10.13145/bacdive5055.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Plesiomonas
  • species: Plesiomonas shigelloides
  • full scientific name: Plesiomonas shigelloides corrig. (Bader 1954) Habs and Schubert 1962 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Pseudomonas shigelloides
    20215Pleisomonas shigelloides

@ref: 3211

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacterales, unclassified

genus: Plesiomonas

species: Plesiomonas shigelloides

full scientific name: Plesiomonas shigelloides (Bader 1954) Habs and Schubert 1962

strain designation: M51, Bader M51

type strain: yes

Morphology

cell morphology

  • @ref: 122586
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3211R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
35009MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122586CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
3211positivegrowth28mesophilic
35009positivegrowth30mesophilic
44190positivegrowth37mesophilic
122586positivegrowth30-41
122586nogrowth5psychrophilic
122586nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44190aerobe
122586facultative anaerobe

spore formation

  • @ref: 122586
  • spore formation: no

compound production

  • @ref: 3211
  • compound: tetrodotoxin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
12258629864mannitol+fermentation
12258616947citrate-carbon source
12258617234glucose+fermentation
12258617716lactose-fermentation
12258617632nitrate+reduction
12258616301nitrite-reduction
12258615792malonate-assimilation
122586132112sodium thiosulfate-builds gas from
12258617234glucose+degradation

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno
12258635581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole+
12258615688acetoin-
12258617234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
122586oxidase+
122586beta-galactosidase+3.2.1.23
122586alcohol dehydrogenase+1.1.1.1
122586gelatinase-
122586catalase+1.11.1.6
122586lysine decarboxylase+4.1.1.18
122586ornithine decarboxylase+4.1.1.17
122586tryptophan deaminase-
122586urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44190C14:03.614
    44190C16:030.316
    44190C18:01.418
    44190C11:0 ISO 3OH512.09
    44190C12:1 3OH3.613.289
    44190C13:0 ISO 2OH0.813.814
    44190C14:0 3OH/C16:1 ISO I4.115.485
    44190C16:0 3OH0.617.52
    44190C16:1 ω7c15.715.819
    44190C16:1 ω9c1.615.774
    44190C17:0 CYCLO17.916.888
    44190C18:1 2OH1.319.088
    44190C18:1 ω5c0.317.919
    44190C18:1 ω7c /12t/9t9.717.824
    44190C18:1 ω9c217.769
    44190C19:0 CYCLO ω8c0.918.9
    44190C19:0 ISO0.418.633
    44190unknown 12.4860.612.486
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122586-+-+-+----++---+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
3211-+++----++-+-+------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
3211dog faecesGermanyDEUEurope
44190Dog ?1955
122586Animal, Dog, fecesGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1154.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_676;97_782;98_906;99_1154&stattab=map
  • Last taxonomy: Plesiomonas shigelloides subclade
  • 16S sequence: X74688
  • Sequence Identity:
  • Total samples: 11067
  • soil counts: 630
  • aquatic counts: 1680
  • animal counts: 7865
  • plant counts: 892

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
3211yesyes2Risk group (German classification)
1225862Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Plesiomonas shigelloides 16S rRNAD11185211ena703
20218Plesiomonas shigelloides 16S rRNAD11234213ena703
20218Plesiomonas shigelloides 16S rRNAD11283200ena703
20218Plesiomonas shigelloides 16S ribosomal RNAM591591489ena703
20218P.shigelloides (ATCC 14029T) gene for 16S ribosomal RNAX746881457ena703
20218Plesiomonas shigelloides strain DSM 8224 16S ribosomal RNA gene, partial sequenceHM0075721348ena703
20218P.shigelloides genes for 16S rRNA (partial) tRNA-Glu, and 16S rRNA (parital)X65486480ena703
3211Plesiomonas shigelloides 16S rRNA gene, strain NCIMB9242X604181499ena703

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Plesiomonas shigelloides NCTC10360GCA_900087055completencbi703
66792Plesiomonas shigelloides strain NCTC10360703.8completepatric703
66792Plesiomonas shigelloides NCTC 103602773857999completeimg703

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes93.894no
flagellatedyes82.004no
gram-positiveno98.798no
anaerobicno93.551yes
aerobicyes54.781yes
halophileno79.287no
spore-formingno94.616no
glucose-fermentyes89.781yes
thermophileno98.53yes
glucose-utilyes85.14no

External links

@ref: 3211

culture collection no.: DSM 8224, ATCC 14029, NCIB 9242, RH 798, CCUG 410, NCTC 10360, NCIMB 9242, CDC 3085-55, CIP 63.5, LMG 4242

straininfo link

  • @ref: 74550
  • straininfo: 3853

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3816787Chemical characterization of enterobacterial common antigen isolated from Plesiomonas shigelloides ATCC 14029.Basu S, Kuhn HM, Neszmelyi A, Himmelspach K, Mayer HEur J Biochem10.1111/j.1432-1033.1987.tb10544.x1987Antigens, Bacterial/*isolation & purification, Carbohydrates/isolation & purification, Fatty Acids/analysis, Hydrogen-Ion Concentration, Hydrolysis, Magnetic Resonance Spectroscopy, Mass Spectrometry, Methylation, Vibrionaceae/*immunologyPhylogeny
6320723Inclusion bodies in Plesiomonas shigelloides.Pastian MR, Bromel MCAppl Environ Microbiol10.1128/aem.47.1.216-218.19841984Inclusion Bodies/*ultrastructure, Microscopy, Electron, Vibrionaceae/*ultrastructure
Genetics27660796Complete Genome Sequence of Plesiomonas shigelloides Type Strain NCTC10360.Alexander S, Fazal MA, Burnett E, Deheer-Graham A, Oliver K, Holroyd N, Parkhill J, Russell JEGenome Announc10.1128/genomeA.01031-162016
Genetics30627119Pathogenomics of Virulence Traits of Plesiomonas shigelloides That Were Deemed Inconclusive by Traditional Experimental Approaches.Ekundayo TC, Okoh AIFront Microbiol10.3389/fmicb.2018.030772018
35097270Ciprofloxacin-Tethered 1,2,3-Triazole Conjugates: New Quinolone Family Compounds to Upgrade Our Antiquated Approach against Bacterial Infections.Agarwal A, Singh P, Maurya A, Patel UK, Singh A, Nath GACS Omega10.1021/acsomega.1c053032022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3211Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8224)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8224
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35009Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10010
44190Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 410)https://www.ccug.se/strain?id=410
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74550Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3853.1StrainInfo: A central database for resolving microbial strain identifiers
122586Curators of the CIPCollection of Institut Pasteur (CIP 63.5)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2063.5