Strain identifier
BacDive ID: 502
Type strain: 
Species: Anaerolinea thermolimosa
Strain Designation: IMO-1
Strain history: T. Yamada IMO-1.
NCBI tax ID(s): 229919 (species)
version 8.1 (current version)
General
@ref: 6506
BacDive-ID: 502
DSM-Number: 16554
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, filament-shaped
description: Anaerolinea thermolimosa IMO-1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from sludge from a thermophilic UASB reactor.
NCBI tax id
- NCBI tax id: 229919
- Matching level: species
strain history
| @ref | history |
|---|---|
| 6506 | <- T. Yamada, Nagaoka Univ. Technology, Japan; IMO-1 <- T. Yamada {2001} |
| 67770 | T. Yamada IMO-1. |
doi: 10.13145/bacdive502.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Anaerolineae
- order: Anaerolineales
- family: Anaerolineaceae
- genus: Anaerolinea
- species: Anaerolinea thermolimosa
- full scientific name: Anaerolinea thermolimosa Yamada et al. 2006
@ref: 6506
domain: Bacteria
phylum: Chloroflexi
class: Anaerolineae
order: Anaerolineales
family: Anaerolineaceae
genus: Anaerolinea
species: Anaerolinea thermolimosa
full scientific name: Anaerolinea thermolimosa Yamada et al. 2006 emend. Nouioui et al. 2018
strain designation: IMO-1
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 23356 | negative | >100 µm | 0.3-0.4 µm | filament-shaped | no | |
| 69480 | negative | 99.579 |
Culture and growth conditions
culture medium
- @ref: 6506
- name: ANAEROLINEA MEDIUM (DSMZ Medium 1004)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1004
- composition: Name: ANAEROLINEA MEDIUM (DSMZ Medium 1004; with strain-specific modifications) Composition: Sucrose 7.17846 g/l NaHCO3 2.49252 g/l NH4Cl 0.538385 g/l Na2S x 9 H2O 0.249252 g/l L-Cysteine HCl x H2O 0.249252 g/l MgCl2 x 6 H2O 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l KH2PO4 0.139581 g/l Yeast extract 0.0997009 g/l Na2-EDTA x 2 H2O 0.00518445 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 6506 | positive | growth | 50 | thermophilic | |
| 23356 | positive | maximum | 55 | thermophilic | |
| 23356 | positive | minimum | 42 | thermophilic | |
| 23356 | positive | optimum | 50 | thermophilic | |
| 67770 | positive | growth | 50 | thermophilic | |
| 69480 | thermophilic | 96.162 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 23356 | positive | minimum | 6.0 |
| 23356 | positive | maximum | 7.5 |
| 23356 | positive | optimum | 7.0 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 6506 | anaerobe | |
| 23356 | anaerobe | |
| 69480 | anaerobe | 93.398 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 94 |
| 69480 | no | 98.804 |
metabolite utilization
- @ref: 23356
- metabolite: yeast extract
- utilization activity: +
- kind of utilization tested: required for growth
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 6506 | sludge from a thermophilic UASB reactor | Niigata, Nagaoka | Japan | JPN | Asia |
| 23356 | sludge from a thermophilic UASB reactor, in wastewater from manufacture of a Japanese distilled alcohol | ||||
| 67770 | Granular sludge collected from a UASB reactor operated under a thermophilic condition (55C) |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | #Anaerobic digestor |
| #Engineered | #Waste | #Wastewater |
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_7302.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_405;96_3416;97_4193;98_5358;99_7302&stattab=map
- Last taxonomy: Anaerolinea thermolimosa subclade
- 16S sequence: AB109437
- Sequence Identity:
- Total samples: 916
- soil counts: 137
- aquatic counts: 434
- animal counts: 320
- plant counts: 25
Safety information
risk assessment
- @ref: 6506
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6506
- description: Anaerolinea thermolimosa gene for 16S rRNA, partial sequence
- accession: AB109437
- length: 1428
- database: ena
- NCBI tax ID: 229919
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Anaerolinea thermolimosa strain IMO-1 | 229919.9 | wgs | patric | 229919 |
| 67770 | Anaerolinea thermolimosa IMO-1 | GCA_001050195 | scaffold | ncbi | 229919 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 6506 | 53.3 | |
| 23356 | 53.3 | high performance liquid chromatography (HPLC) |
| 67770 | 54.6 | genome sequence analysis |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 94 | no |
| motile | no | 56.075 | no |
| gram-positive | no | 95.387 | yes |
| anaerobic | yes | 70.848 | no |
| aerobic | no | 92.273 | yes |
| halophile | no | 95.879 | no |
| spore-forming | no | 82.816 | no |
| glucose-util | yes | 85.091 | no |
| flagellated | no | 89.917 | no |
| thermophile | yes | 98.119 | yes |
| glucose-ferment | no | 75.153 | no |
External links
@ref: 6506
culture collection no.: DSM 16554, JCM 12577
straininfo link
- @ref: 70181
- straininfo: 297125
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16738111 | Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi. | Yamada T, Sekiguchi Y, Hanada S, Imachi H, Ohashi A, Harada H, Kamagata Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.64169-0 | 2006 | Chloroflexi/*classification/genetics/growth & development/isolation & purification, Culture Media, Microscopy, Phase-Contrast, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics | Cultivation |
| Genetics | 26383658 | Draft Genome Sequences of Anaerolinea thermolimosa IMO-1, Bellilinea caldifistulae GOMI-1, Leptolinea tardivitalis YMTK-2, Levilinea saccharolytica KIBI-1, Longilinea arvoryzae KOME-1, Previously Described as Members of the Class Anaerolineae (Chloroflexi). | Matsuura N, Tourlousse DM, Ohashi A, Hugenholtz P, Sekiguchi Y | Genome Announc | 10.1128/genomeA.00975-15 | 2015 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 6506 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16554) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16554 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 23356 | Takeshi Yamada, Yuji Sekiguchi, Satoshi Hanada, Hiroyuki Imachi, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata | 10.1099/ijs.0.64169-0 | Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi | IJSEM 56: 1331-1340 2006 | 16738111 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 70181 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297125.1 | StrainInfo: A central database for resolving microbial strain identifiers |