Strain identifier

BacDive ID: 4983

Type strain: Yes

Species: Moellerella wisconsensis

Strain Designation: 2896-78

Strain history: CIP <- 1987, ATCC <- CDC: strain 2896-78 <- 1978, Wisconsin Ste. Hlth. Hosp., USA

NCBI tax ID(s): 1354267 (strain), 158849 (species)

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General

@ref: 1923

BacDive-ID: 4983

DSM-Number: 5076

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Moellerella wisconsensis 2896-78 is an aerobe, mesophilic, motile bacterium of the family Morganellaceae.

NCBI tax id

NCBI tax idMatching level
1354267strain
158849species

strain history

@refhistory
1923<- ATCC
67770Y. Kosako 85561 <-- R. Sakazaki 85023 <-- CDC 2896-78.
123951CIP <- 1987, ATCC <- CDC: strain 2896-78 <- 1978, Wisconsin Ste. Hlth. Hosp., USA

doi: 10.13145/bacdive4983.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Morganellaceae
  • genus: Moellerella
  • species: Moellerella wisconsensis
  • full scientific name: Moellerella wisconsensis Hickman-Brenner et al. 1984

@ref: 1923

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Morganellaceae

genus: Moellerella

species: Moellerella wisconsensis

full scientific name: Moellerella wisconsensis Hickman-Brenner et al. 1984

strain designation: 2896-78

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes92.82
6948099.858negative
123951negativerod-shaped

colony morphology

  • @ref: 59366
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1923NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
40513MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123951CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123951CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1923positivegrowth37mesophilic
40513positivegrowth30mesophilic
59366positivegrowth37mesophilic
67770positivegrowth37mesophilic
123951positivegrowth10-37
123951nogrowth5psychrophilic
123951nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59366aerobe
123951facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.975

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
123951mannitol+fermentation29864
123951citrate+carbon source16947
123951glucose+fermentation17234
123951lactose+fermentation17716
123951nitrate+reduction17632
123951nitrite-reduction16301
123951malonate-assimilation15792
123951sodium thiosulfate-builds gas from132112

antibiotic resistance

  • @ref: 123951
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123951
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12395115688acetoin-
12395117234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123951oxidase-
123951beta-galactosidase+3.2.1.23
123951alcohol dehydrogenase-1.1.1.1
123951gelatinase-
123951catalase+1.11.1.6
123951lysine decarboxylase-4.1.1.18
123951ornithine decarboxylase-4.1.1.17
123951phenylalanine ammonia-lyase-4.3.1.24
123951tryptophan deaminase-
123951urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    59366C14:08.514
    59366C15:05.515
    59366C16:031.716
    59366C17:00.917
    59366C13:0 3OH/C15:1 i I/H0.314.469
    59366C14:0 3OH/C16:1 ISO I5.615.485
    59366C16:1 ω5c0.415.908
    59366C16:1 ω7c4.415.819
    59366C17:0 CYCLO35.516.888
    59366C18:1 ω7c /12t/9t4.917.824
    59366C19:0 CYCLO ω8c0.418.9
    59366Unidentified0.616.094
    59366Unidentified0.617.755
    59366Unidentified0.717.873
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123951-+++-++-+-++++-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123951+/----+--+-++++----+/-+/---++/-+/-+/---+/-+++--+/-+--+/-+-----+-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123951+++-+-+++--++++-++-+------+----+-+---+----------++++-+-----++--------------+---++--------+++--+--+-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typesampling dateisolation date
40513WisconsinUSAUSANorth America
59366WisconsinUSAUSANorth AmericaHuman feces1978
67770Stool
123951WisconsinUnited States of AmericaUSANorth AmericaHuman, Stool1978

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5540.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_362;97_407;98_4135;99_5540&stattab=map
  • Last taxonomy: Providencia
  • 16S sequence: JN175344
  • Sequence Identity:
  • Total samples: 11469
  • soil counts: 703
  • aquatic counts: 1133
  • animal counts: 9438
  • plant counts: 195

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
19232Risk group (German classification)
1239511Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Moellerella wisconsensis strain CIP 103034 16S ribosomal RNA gene, complete sequenceJN1753441532ena158849
67770Moellerella wisconsensis 16S rRNA gene, type strain DSM 5676TAM0407541482ena158849

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moellerella wisconsensis ATCC 350171354267.4wgspatric1354267
66792Moellerella wisconsensis strain NCTC12132158849.7wgspatric158849
66792Moellerella wisconsensis ATCC 350172636415824draftimg1354267
67770Moellerella wisconsensis NCTC12132GCA_900706815contigncbi158849
67770Moellerella wisconsensis ATCC 35017GCA_001294465contigncbi1354267

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes82.968no
gram-positiveno98.21no
anaerobicno99.137yes
halophileno77.232no
spore-formingno96.918no
thermophileno98.612no
glucose-utilyes89.868no
flagellatedno57.487no
aerobicyes88.255yes
glucose-fermentyes90.164no

External links

@ref: 1923

culture collection no.: DSM 5076, ATCC 35017, CCUG 51928, NCTC 12132, LMG 10145, JCM 5895, CDC 2896-78, CIP 103034

straininfo link

  • @ref: 74479
  • straininfo: 92349

literature

  • topic: Phylogeny
  • Pubmed-ID: 6715516
  • title: Moellerella wisconsensis, a new genus and species of Enterobacteriaceae found in human stool specimens.
  • authors: Hickman-Brenner FW, Huntley-Carter GP, Saitoh Y, Steigerwalt AG, Farmer JJ 3rd, Brenner DJ
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.19.4.460-463.1984
  • year: 1984
  • mesh: Adult, Anti-Bacterial Agents/pharmacology, Child, Preschool, DNA, Bacterial/analysis, Diarrhea/microbiology, Enterobacteriaceae/*classification/drug effects/genetics/isolation & purification/metabolism, Feces/*microbiology, Female, Gastroenteritis/microbiology, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Nucleic Acid Hybridization
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1923Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5076)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5076
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40513Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14786
59366Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51928)https://www.ccug.se/strain?id=51928
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74479Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92349.1StrainInfo: A central database for resolving microbial strain identifiers
123951Curators of the CIPCollection of Institut Pasteur (CIP 103034)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103034