Strain identifier

BacDive ID: 4973

Type strain: Yes

Species: Kluyvera ascorbata

Strain Designation: 0648-74

Strain history: CIP <- 1982, ATCC <- CDC: strain 0648-74

NCBI tax ID(s): 1006000 (strain), 51288 (species)

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General

@ref: 1801

BacDive-ID: 4973

DSM-Number: 4611

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Kluyvera ascorbata 0648-74 is an aerobe, mesophilic bacterium that was isolated from human sputum.

NCBI tax id

NCBI tax idMatching level
1006000strain
51288species

strain history

@refhistory
1801<- CIP <- ATCC
67770IAM 14203 <-- GIFU 10603 <-- ATCC 33433 <-- CDC 0648-74 <-- North Carolina State Health Dept., USA.
116523CIP <- 1982, ATCC <- CDC: strain 0648-74

doi: 10.13145/bacdive4973.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Kluyvera
  • species: Kluyvera ascorbata
  • full scientific name: Kluyvera ascorbata Farmer et al. 1981

@ref: 1801

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Kluyvera

species: Kluyvera ascorbata

full scientific name: Kluyvera ascorbata Farmer et al. 1981

strain designation: 0648-74

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1801COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1801NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37877MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116523CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1801positivegrowth30mesophilic
37877positivegrowth30mesophilic
46329positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 46329
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
1801+/---++---+--++--+++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1801human sputum
67770Human sputumNCUSAUSANorth America
116523SputumNorth CarolinaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Sputum

taxonmaps

  • @ref: 69479
  • File name: preview.99_6966.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_1600;98_1946;99_6966&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: AF310219
  • Sequence Identity:
  • Total samples: 6808
  • soil counts: 165
  • aquatic counts: 508
  • animal counts: 5631
  • plant counts: 504

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
18012Risk group (German classification)
1165231Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Kluyvera ascorbata ATCC 33433 16S ribosomal RNA gene, partial sequenceAF0085791459ena1006000
20218Kluyvera ascorbata 16S ribosomal RNA gene, partial sequenceAF3102191437ena1006000
20218Kluyvera ascorbata gene for 16S rRNA, partial sequence, strain: NBRC 102466AB6818081465ena51288
1801Kluyvera ascorbata strain NBRC 102466 16S ribosomal RNA, partial sequenceNR_1141071465nuccore51288

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kluyvera ascorbata ATCC 334331006000.3wgspatric1006000
66792Kluyvera ascorbata ATCC 334332588253795draftimg1006000
67770Kluyvera ascorbata ATCC 33433GCA_000735365contigncbi1006000

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes86.427no
flagellatedno54.266no
gram-positiveno98.148no
anaerobicno96.173yes
aerobicyes86.367yes
halophileno91.764no
spore-formingno91.488no
thermophileno99.291yes
glucose-utilyes94.156no
glucose-fermentyes90.505no

External links

@ref: 1801

culture collection no.: DSM 4611, ATCC 33433, CDC 0648-74, CIP 82.95, GIFU 10603, IAM 14203, NBRC 102466, CCUG 15716, CECT 861, JCM 21070, LMG 7871

straininfo link

  • @ref: 74469
  • straininfo: 3183

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1801Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4611)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4611
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37877Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11371
46329Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 15716)https://www.ccug.se/strain?id=15716
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74469Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3183.1StrainInfo: A central database for resolving microbial strain identifiers
116523Curators of the CIPCollection of Institut Pasteur (CIP 82.95)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2082.95