Strain identifier
BacDive ID: 4971
Type strain:
Species: Kluyvera intermedia
Strain Designation: E86
Strain history: CIP <- 1979, H. Leclerc, INSERM, Villeneuve-d'Ascq, France: strain CUETM 77-130 <- F. Gavini, INSERM, France: strain E86
NCBI tax ID(s): 1218113 (strain), 61648 (species)
General
@ref: 1779
BacDive-ID: 4971
DSM-Number: 4581
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile
description: Kluyvera intermedia E86 is an aerobe, mesophilic, motile bacterium that was isolated from Water,surface.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1218113 | strain |
61648 | species |
strain history
@ref | history |
---|---|
1779 | <- CIP <- H. Leclerc, CUETM <- F. Gavini, E86 |
67770 | R. Sakazaki 256 <-- H. Leclerc CUETM 77-130 <-- F. Gavini E 86. |
120145 | CIP <- 1979, H. Leclerc, INSERM, Villeneuve-d'Ascq, France: strain CUETM 77-130 <- F. Gavini, INSERM, France: strain E86 |
doi: 10.13145/bacdive4971.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Kluyvera
- species: Kluyvera intermedia
- full scientific name: Kluyvera intermedia (Izard et al. 1980) Pavan et al. 2005
synonyms
@ref synonym 20215 Kluyvera cochleae 20215 Enterobacter intermedium 20215 Enterobacter intermedius
@ref: 1779
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Kluyvera
species: Kluyvera intermedia
full scientific name: Kluyvera intermedia (Izard et al. 1980) Pavan et al. 2005
strain designation: E86
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 95.366 | ||
69480 | 99.981 | negative | ||
120145 | yes | negative | rod-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
1779 | REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) | yes | https://mediadive.dsmz.de/medium/1a | Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
37713 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
120145 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1779 | positive | growth | 30 | mesophilic |
37713 | positive | growth | 30 | mesophilic |
46037 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
120145 | positive | growth | 10-37 | |
120145 | no | growth | 5 | psychrophilic |
120145 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
46037 | aerobe |
120145 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.835 |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | + | builds acid from | |
68371 | gluconate | + | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | + | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | + | builds acid from | 28053 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | + | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | + | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | L-rhamnose | + | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | + | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | + | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
68368 | L-arabinose | + | fermentation | 30849 |
68368 | amygdalin | + | fermentation | 27613 |
68368 | melibiose | + | fermentation | 28053 |
68368 | sucrose | + | fermentation | 17992 |
68368 | L-rhamnose | + | fermentation | 62345 |
68368 | sorbitol | + | fermentation | 30911 |
68368 | myo-inositol | - | fermentation | 17268 |
68368 | D-mannitol | + | fermentation | 16899 |
68368 | D-glucose | + | fermentation | 17634 |
68368 | gelatin | - | hydrolysis | 5291 |
68368 | tryptophan | - | energy source | 27897 |
68368 | urea | - | hydrolysis | 16199 |
68368 | citrate | + | assimilation | 16947 |
68368 | ornithine | + | degradation | 18257 |
68368 | lysine | - | degradation | 25094 |
68368 | arginine | - | hydrolysis | 29016 |
120145 | mannitol | + | fermentation | 29864 |
120145 | citrate | + | carbon source | 16947 |
120145 | glucose | + | fermentation | 17234 |
120145 | lactose | + | fermentation | 17716 |
120145 | nitrate | + | reduction | 17632 |
120145 | nitrite | - | reduction | 16301 |
120145 | malonate | + | assimilation | 15792 |
120145 | sodium thiosulfate | - | builds gas from | 132112 |
120145 | glucose | + | degradation | 17234 |
antibiotic resistance
- @ref: 120145
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
120145 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test | methylred-test |
---|---|---|---|---|---|
68368 | 15688 | acetoin | + | ||
68368 | 35581 | indole | - | ||
120145 | 15688 | acetoin | + | ||
120145 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | + | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
120145 | oxidase | - | |
120145 | beta-galactosidase | + | 3.2.1.23 |
120145 | alcohol dehydrogenase | - | 1.1.1.1 |
120145 | gelatinase | - | |
120145 | catalase | + | 1.11.1.6 |
120145 | lysine decarboxylase | - | 4.1.1.18 |
120145 | ornithine decarboxylase | + | 4.1.1.17 |
120145 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
120145 | tryptophan deaminase | - | |
120145 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120145 | - | + | - | + | - | + | - | - | - | - | + | + | - | + | - | + | - | - | - | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1779 | + | - | - | + | + | - | - | - | - | + | - | + | + | - | + | + | + | + | + | + | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120145 | + | - | - | + | + | + | - | - | - | + | + | + | + | - | + | +/- | - | + | + | - | + | + | +/- | + | + | + | + | + | + | + | + | + | - | - | + | - | - | - | + | +/- | - | +/- | - | +/- | - | - | + | + | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120145 | + | + | + | + | + | - | + | + | + | - | + | + | - | + | + | + | - | + | + | + | + | + | - | + | - | - | - | - | - | + | + | + | - | + | + | - | + | - | - | - | - | + | - | + | + | - | - | - | - | + | - | - | - | + | + | + | + | + | - | + | + | + | + | + | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | + | - | + | - | - | - | + | - | - | + | + | - | - | + | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
46037 | Water,surface |
67770 | Surface water |
120145 | Environment, Surface water |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Surface water
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
1779 | 2 | Risk group (German classification) |
120145 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Enterobacter intermedius 16S ribosomal RNA gene, partial sequence | AF310217 | 1453 | ena | 61648 |
20218 | Kluyvera intermedia gene for 16S ribosomal RNA, partial sequence | AB004747 | 1438 | ena | 61648 |
20218 | Kluyvera intermedia gene for 16S rRNA, partial sequence, strain: NBRC 102594 | AB681871 | 1465 | ena | 61648 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Kluyvera intermedia NCTC12125 | GCA_900635475 | complete | ncbi | 61648 |
66792 | Kluyvera intermedia NBRC 102594 = ATCC 33110 | 1218113.4 | wgs | patric | 1218113 |
66792 | Kluyvera intermedia strain NCTC12125 | 61648.17 | complete | patric | 61648 |
66792 | Kluyvera intermedia NBRC 102594 | 2731957696 | draft | img | 1218113 |
67770 | Kluyvera intermedia NBRC 102594 = ATCC 33110 | GCA_001598315 | contig | ncbi | 1218113 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 53.468 | no |
gram-positive | no | 98.131 | no |
anaerobic | no | 96.597 | yes |
aerobic | yes | 90.123 | yes |
halophile | no | 91.387 | no |
spore-forming | no | 93.824 | no |
thermophile | no | 99.493 | yes |
glucose-util | yes | 91.629 | no |
motile | yes | 85.243 | no |
glucose-ferment | yes | 92.059 | yes |
External links
@ref: 1779
culture collection no.: CCUG 14183, CIP 79-27, NCTC 12125, LMG 2785, DSM 4581, ATCC 33110, CIP 79.27, CUETM 77.130, IAM 14238, JCM 1238, BCRC 14808, CCM 3433, CUETM 77-130, HAMBI 1299, HUT 8114, NBRC 102594, NCIMB 13626, VTT E-97862
straininfo link
- @ref: 74467
- straininfo: 389410
literature
- topic: Phylogeny
- Pubmed-ID: 15653915
- title: Phylogenetic relationships of the genus Kluyvera: transfer of Enterobacter intermedius Izard et al. 1980 to the genus Kluyvera as Kluyvera intermedia comb. nov. and reclassification of Kluyvera cochleae as a later synonym of K. intermedia.
- authors: Pavan ME, Franco RJ, Rodriguez JM, Gadaleta P, Abbott SL, Janda JM, Zorzopulos J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63071-0
- year: 2005
- mesh: Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Enterobacter/*classification/genetics/metabolism, Enterobacteriaceae/classification/genetics/metabolism, Kluyvera/*classification/genetics/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terminology as Topic
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1779 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4581) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4581 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37713 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/11053 | ||||
46037 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 14183) | https://www.ccug.se/strain?id=14183 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
74467 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID389410.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120145 | Curators of the CIP | Collection of Institut Pasteur (CIP 79.27) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2079.27 |