Strain identifier
BacDive ID: 4951
Type strain:
Species: Klebsiella pneumoniae
Strain Designation: 62-1
Strain history: <- ATCC <- F. Gibson, 62-1 (Aerobacter aerogenes)
NCBI tax ID(s): 573 (species)
General
@ref: 2316
BacDive-ID: 4951
DSM-Number: 5758
keywords: Bacteria, mesophilic, human pathogen
description: Klebsiella pneumoniae 62-1 is a mesophilic human pathogen of the family Enterobacteriaceae.
NCBI tax id
- NCBI tax id: 573
- Matching level: species
strain history
- @ref: 2316
- history: <- ATCC <- F. Gibson, 62-1 (Aerobacter aerogenes)
doi: 10.13145/bacdive4951.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Klebsiella
- species: Klebsiella pneumoniae
- full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Hyalococcus pneumoniae
@ref: 2316
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Klebsiella
species: Klebsiella pneumoniae
full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887
strain designation: 62-1
type strain: no
Culture and growth conditions
culture medium
- @ref: 2316
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
- @ref: 2316
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
compound production
@ref | compound |
---|---|
2316 | anthralinat synthetase |
2316 | chorismic acid |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68368 | 30849 | L-arabinose | + | fermentation |
68368 | 27613 | amygdalin | + | fermentation |
68368 | 28053 | melibiose | + | fermentation |
68368 | 17992 | sucrose | + | fermentation |
68368 | 62345 | L-rhamnose | + | fermentation |
68368 | 30911 | sorbitol | + | fermentation |
68368 | 17268 | myo-inositol | + | fermentation |
68368 | 16899 | D-mannitol | + | fermentation |
68368 | 17634 | D-glucose | + | fermentation |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | + | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2316 | + | - | + | - | - | - | - | - | - | + | - | + | + | + | + | + | + | + | + | + | - |
Safety information
risk assessment
- @ref: 2316
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 2316
culture collection no.: DSM 5758, ATCC 25306, DSM 1470
straininfo link
- @ref: 74447
- straininfo: 38478
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 1750676 | Bulk purification of isochorismic acid by low-pressure octadecyl (C18) reverse-phase liquid chromatography. | Seeger JW Jr, Bentley R | Anal Biochem | 10.1016/0003-2697(91)90326-o | 1991 | Chorismic Acid/*isolation & purification, Chromatography, Liquid/*methods, Cyclohexenes, *Intramolecular Transferases, Isomerases/pharmacology, Isomerism, Klebsiella pneumoniae/enzymology | Phylogeny |
Metabolism | 3300552 | Biosynthesis of o-succinylbenzoic acid. I: Cell free synthesis of o-succinylbenzoic acid from isochorismic acid in enzyme preparations from vitamin K producing bacteria. | Weische A, Johanni M, Leistner E | Arch Biochem Biophys | 10.1016/0003-9861(87)90439-5 | 1987 | *Carbon-Carbon Lyases, Chorismic Acid/*metabolism, Chromatography, High Pressure Liquid, Cyclohexanecarboxylic Acids/*metabolism, Cyclohexenes, Enterobacteriaceae/enzymology/*metabolism, Escherichia coli/metabolism, Ketoglutarate Dehydrogenase Complex/metabolism, Ketoglutaric Acids/metabolism, Lyases/metabolism, Mutation, Phenylbutyrates/*biosynthesis, Vitamin K/*biosynthesis | Enzymology |
Enzymology | 6381056 | 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 1. Purification and properties. | Steinrucken HC, Amrhein N | Eur J Biochem | 10.1111/j.1432-1033.1984.tb08378.x | 1984 | 3-Phosphoshikimate 1-Carboxyvinyltransferase, *Alkyl and Aryl Transferases, Anions/pharmacology, Binding Sites, Cations/pharmacology, Chemical Phenomena, Chemistry, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Isoelectric Focusing, Kinetics, Klebsiella pneumoniae/*enzymology/growth & development, Molecular Weight, Substrate Specificity, Temperature, Transferases/antagonists & inhibitors/*isolation & purification | Phylogeny |
Metabolism | 8590665 | Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid. | Muller R, Wagener A, Schmidt K, Leistner E | Appl Microbiol Biotechnol | 10.1007/BF00166913 | 1995 | Anthranilate Synthase/genetics/*metabolism, Bacterial Proteins/genetics/*metabolism, Base Sequence, Carbon Isotopes, Escherichia coli/enzymology/genetics, Genetic Vectors, Glucose/chemistry, Isotope Labeling, Klebsiella pneumoniae/*metabolism, Molecular Sequence Data, Oxo-Acid-Lyases/genetics/*metabolism, Parabens/chemistry/*isolation & purification/metabolism, Recombinant Proteins/metabolism, Transformation, Bacterial | Enzymology |
Enzymology | 8744423 | Small scale biosynthesis and purification of gram quantities of chorismic acid. | Rieger CE, Turnbull JL | Prep Biochem Biotechnol | 10.1080/10826069608000051 | 1996 | Chorismic Acid/*isolation & purification/*metabolism, Crystallization, Culture Media, Conditioned, Drug Stability, Hydrogen-Ion Concentration, Klebsiella pneumoniae/growth & development/*metabolism | Metabolism |
Metabolism | 8936326 | Bacterial production of transdihydroxycyclohexadiene carboxylates by metabolic pathway engineering. | Muller R, Breuer M, Wagener A, Schmidt K, Leistner E | Microbiology (Reading) | 10.1099/00221287-142-4-1005 | 1996 | Amino Acid Sequence, Base Sequence, Carboxylic Acids/chemistry/*metabolism, Chorismic Acid/metabolism, Cyclohexanols/chemistry/*metabolism, Cyclohexenes, DNA Primers/genetics, DNA, Bacterial/genetics, Escherichia coli/genetics, Genes, Bacterial, *Genetic Engineering, Hydrolases/genetics/metabolism, *Intramolecular Transferases, Iron/metabolism, Isomerases/genetics/metabolism, Klebsiella pneumoniae/*genetics/*metabolism, Molecular Sequence Data, Mutation, Plasmids/genetics, Stereoisomerism, Transformation, Genetic | Biotechnology |
Metabolism | 9795253 | The role of isochorismate hydroxymutase genes entC and menF in enterobactin and menaquinone biosynthesis in Escherichia coli. | Dahm C, Muller R, Schulte G, Schmidt K, Leistner E | Biochim Biophys Acta | 10.1016/s0304-4165(98)00089-0 | 1998 | Chorismic Acid/metabolism, Cyclohexenes, Enterobactin/*metabolism, Escherichia coli/enzymology/*genetics, Gene Expression, Intramolecular Transferases/*genetics/metabolism, Klebsiella pneumoniae/genetics, Mutation, Plasmids, Vitamin K/*biosynthesis | Enzymology |
Biotechnology | 18623181 | Microbial production of (+)-trans-isochorismic acid. | Schmidt K, Leistner E | Biotechnol Bioeng | 10.1002/bit.260450402 | 1995 |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
2316 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5758) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5758 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
68368 | Automatically annotated from API 20E | |||
74447 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID38478.1 | StrainInfo: A central database for resolving microbial strain identifiers |