Strain identifier

BacDive ID: 4951

Type strain: No

Species: Klebsiella pneumoniae

Strain Designation: 62-1

Strain history: <- ATCC <- F. Gibson, 62-1 (Aerobacter aerogenes)

NCBI tax ID(s): 573 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2316

BacDive-ID: 4951

DSM-Number: 5758

keywords: Bacteria, mesophilic, human pathogen

description: Klebsiella pneumoniae 62-1 is a mesophilic human pathogen of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 573
  • Matching level: species

strain history

  • @ref: 2316
  • history: <- ATCC <- F. Gibson, 62-1 (Aerobacter aerogenes)

doi: 10.13145/bacdive4951.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae
  • full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Hyalococcus pneumoniae

@ref: 2316

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae

full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887

strain designation: 62-1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2316
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

  • @ref: 2316
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

compound production

@refcompound
2316anthralinat synthetase
2316chorismic acid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
2316+-+------+-+++++++++-

Safety information

risk assessment

  • @ref: 2316
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 2316

culture collection no.: DSM 5758, ATCC 25306, DSM 1470

straininfo link

  • @ref: 74447
  • straininfo: 38478

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1750676Bulk purification of isochorismic acid by low-pressure octadecyl (C18) reverse-phase liquid chromatography.Seeger JW Jr, Bentley RAnal Biochem10.1016/0003-2697(91)90326-o1991Chorismic Acid/*isolation & purification, Chromatography, Liquid/*methods, Cyclohexenes, *Intramolecular Transferases, Isomerases/pharmacology, Isomerism, Klebsiella pneumoniae/enzymologyPhylogeny
Metabolism3300552Biosynthesis of o-succinylbenzoic acid. I: Cell free synthesis of o-succinylbenzoic acid from isochorismic acid in enzyme preparations from vitamin K producing bacteria.Weische A, Johanni M, Leistner EArch Biochem Biophys10.1016/0003-9861(87)90439-51987*Carbon-Carbon Lyases, Chorismic Acid/*metabolism, Chromatography, High Pressure Liquid, Cyclohexanecarboxylic Acids/*metabolism, Cyclohexenes, Enterobacteriaceae/enzymology/*metabolism, Escherichia coli/metabolism, Ketoglutarate Dehydrogenase Complex/metabolism, Ketoglutaric Acids/metabolism, Lyases/metabolism, Mutation, Phenylbutyrates/*biosynthesis, Vitamin K/*biosynthesisEnzymology
Enzymology63810565-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 1. Purification and properties.Steinrucken HC, Amrhein NEur J Biochem10.1111/j.1432-1033.1984.tb08378.x19843-Phosphoshikimate 1-Carboxyvinyltransferase, *Alkyl and Aryl Transferases, Anions/pharmacology, Binding Sites, Cations/pharmacology, Chemical Phenomena, Chemistry, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Isoelectric Focusing, Kinetics, Klebsiella pneumoniae/*enzymology/growth & development, Molecular Weight, Substrate Specificity, Temperature, Transferases/antagonists & inhibitors/*isolation & purificationPhylogeny
Metabolism8590665Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid.Muller R, Wagener A, Schmidt K, Leistner EAppl Microbiol Biotechnol10.1007/BF001669131995Anthranilate Synthase/genetics/*metabolism, Bacterial Proteins/genetics/*metabolism, Base Sequence, Carbon Isotopes, Escherichia coli/enzymology/genetics, Genetic Vectors, Glucose/chemistry, Isotope Labeling, Klebsiella pneumoniae/*metabolism, Molecular Sequence Data, Oxo-Acid-Lyases/genetics/*metabolism, Parabens/chemistry/*isolation & purification/metabolism, Recombinant Proteins/metabolism, Transformation, BacterialEnzymology
Enzymology8744423Small scale biosynthesis and purification of gram quantities of chorismic acid.Rieger CE, Turnbull JLPrep Biochem Biotechnol10.1080/108260696080000511996Chorismic Acid/*isolation & purification/*metabolism, Crystallization, Culture Media, Conditioned, Drug Stability, Hydrogen-Ion Concentration, Klebsiella pneumoniae/growth & development/*metabolismMetabolism
Metabolism8936326Bacterial production of transdihydroxycyclohexadiene carboxylates by metabolic pathway engineering.Muller R, Breuer M, Wagener A, Schmidt K, Leistner EMicrobiology (Reading)10.1099/00221287-142-4-10051996Amino Acid Sequence, Base Sequence, Carboxylic Acids/chemistry/*metabolism, Chorismic Acid/metabolism, Cyclohexanols/chemistry/*metabolism, Cyclohexenes, DNA Primers/genetics, DNA, Bacterial/genetics, Escherichia coli/genetics, Genes, Bacterial, *Genetic Engineering, Hydrolases/genetics/metabolism, *Intramolecular Transferases, Iron/metabolism, Isomerases/genetics/metabolism, Klebsiella pneumoniae/*genetics/*metabolism, Molecular Sequence Data, Mutation, Plasmids/genetics, Stereoisomerism, Transformation, GeneticBiotechnology
Metabolism9795253The role of isochorismate hydroxymutase genes entC and menF in enterobactin and menaquinone biosynthesis in Escherichia coli.Dahm C, Muller R, Schulte G, Schmidt K, Leistner EBiochim Biophys Acta10.1016/s0304-4165(98)00089-01998Chorismic Acid/metabolism, Cyclohexenes, Enterobactin/*metabolism, Escherichia coli/enzymology/*genetics, Gene Expression, Intramolecular Transferases/*genetics/metabolism, Klebsiella pneumoniae/genetics, Mutation, Plasmids, Vitamin K/*biosynthesisEnzymology
Biotechnology18623181Microbial production of (+)-trans-isochorismic acid.Schmidt K, Leistner EBiotechnol Bioeng10.1002/bit.2604504021995

Reference

@idauthorscataloguedoi/urltitle
2316Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5758)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5758
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68368Automatically annotated from API 20E
74447Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38478.1StrainInfo: A central database for resolving microbial strain identifiers