Strain identifier

BacDive ID: 4950

Type strain: No

Species: Klebsiella pneumoniae

Strain Designation: PZH

Strain history: <- ATCC <- Z. Schneider, PZH, 572 (Enterobacter aerogenes) <- B. Zagalak, PZH

NCBI tax ID(s): 1316938 (strain), 573 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1826

BacDive-ID: 4950

DSM-Number: 4799

keywords: genome sequence, Bacteria, mesophilic, Gram-negative, human pathogen

description: Klebsiella pneumoniae PZH is a mesophilic, Gram-negative human pathogen of the family Enterobacteriaceae.

NCBI tax id

NCBI tax idMatching level
1316938strain
573species

strain history

  • @ref: 1826
  • history: <- ATCC <- Z. Schneider, PZH, 572 (Enterobacter aerogenes) <- B. Zagalak, PZH

doi: 10.13145/bacdive4950.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae
  • full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Hyalococcus pneumoniae

@ref: 1826

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae

full scientific name: Klebsiella pneumoniae (Schroeter 1886) Trevisan 1887

strain designation: PZH

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.974

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1826COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1826NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 1826
  • growth: positive
  • type: growth
  • temperature: 25
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.948

compound production

  • @ref: 1826
  • compound: glycerol dehydrase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
1826+-+-+----+-+++++++++-
1826+-+-+-+--+-+++++++++-

Safety information

risk assessment

  • @ref: 1826
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Klebsiella pneumoniae ATCC 25955GCA_000409715contigncbi1316938
66792Klebsiella pneumoniae ATCC 259551316938.3wgspatric1316938
66792Klebsiella pneumoniae ATCC 259552554235128draftimg1316938

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno85.111no
gram-positiveno97.212no
anaerobicno96.399no
halophileno87.614no
spore-formingno93.471no
glucose-utilyes93.864no
flagellatedno92.695no
thermophileno99.64no
aerobicyes90.944no
glucose-fermentyes91.204yes

External links

@ref: 1826

culture collection no.: DSM 4799, ATCC 25955, PZH 572

straininfo link

  • @ref: 74446
  • straininfo: 3120

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology407082Immunochemical evidence for the difference between coenzyme-B12-dependent diol dehydratase and glycerol dehydratase.Toraya T, Fukui SEur J Biochem10.1111/j.1432-1033.1977.tb11594.x1977Cations, Monovalent, Cobamides, Culture Media, Enterobacteriaceae/enzymology, Glycerol/pharmacology, Hydro-Lyases/*metabolism, Immunodiffusion, Kinetics, Klebsiella pneumoniae/drug effects/*enzymology, Propanediol Dehydratase/*metabolism, Propylene Glycols/pharmacology, Species SpecificityCultivation
719056[Effect of the structure of the nucleoside ligand of cobalamines on their enzymatic properties in a glycerol dehydratase system].Iakusheva MI, Gurevich VM, Poznanskaia AA, Iakovlev VA, Khogenkamp GBiokhimiia1978Chemical Phenomena, Chemistry, Circular Dichroism, *Cobamides, Glycerol, *Hydro-Lyases, Ligands, Nucleosides, Structure-Activity Relationship
Metabolism17859291,3-Propanediol production by Escherichia coli expressing genes from the Klebsiella pneumoniae dha regulon.Tong IT, Liao HH, Cameron DCAppl Environ Microbiol10.1128/aem.57.12.3541-3546.19911991Anaerobiosis, Blotting, Southern, Cloning, Molecular, Cosmids, Dihydroxyacetone/metabolism, Escherichia coli/*genetics/metabolism, Gas Chromatography-Mass Spectrometry, Gene Expression Regulation, Bacterial, Glycerol/metabolism, Kinetics, Klebsiella pneumoniae/*genetics/metabolism, Propylene Glycols/*metabolism, Restriction Mapping, Transformation, BacterialEnzymology
Enzymology6753954[Coenzyme properties of adenosylcobalamin analogs with modifications in the purine nucleus of the alpha-ligand].Iakusheva MI, Gurevich VM, Poznanskaia AABiokhimiia1982Cobamides/*pharmacology, Hydro-Lyases/*metabolism, Kinetics, Klebsiella pneumoniae/enzymology, Protein Binding, Structure-Activity RelationshipMetabolism
Metabolism7035429Glycerol fermentation in Klebsiella pneumoniae: functions of the coenzyme B12-dependent glycerol and diol dehydratases.Forage RG, Foster MAJ Bacteriol10.1128/jb.149.2.413-419.19821982Alcohol Oxidoreductases/metabolism, Cobalt/pharmacology, Cobamides/pharmacology, Dihydroxyacetone, Enzyme Induction, Fermentation, Glycerol/*metabolism, Hydro-Lyases/*metabolism, Klebsiella pneumoniae/*enzymology/genetics, Mutation, Propanediol Dehydratase/*metabolismEnzymology
Metabolism12018293Production of 1,3-propanediol by Klebsiella pneumoniae.Huang H, Gong CS, Tsao GTAppl Biochem Biotechnol2002Aerobiosis, Anaerobiosis, Biotechnology/methods, Fermentation, Food Analysis, Glycerol/metabolism, Kinetics, Klebsiella pneumoniae/growth & development/*metabolism, Lubrication, Models, Biological, Polymers, Propylene Glycols/*metabolismBiotechnology
Metabolism19921491Microbial fed-batch production of 1,3-propanediol using raw glycerol with suspended and immobilized Klebsiella pneumoniae.Jun SA, Moon C, Kang CH, Kong SW, Sang BI, Um YAppl Biochem Biotechnol10.1007/s12010-009-8839-x2010Biofuels, Bioreactors, Fermentation, Glycerol/*metabolism, Industrial Microbiology/*methods, Klebsiella pneumoniae/cytology/*metabolism, Propylene Glycols/*metabolismBiotechnology
Pathogenicity22474970Essential oil composition and antibacterial activity of Monticalia greenmaniana (Asteraceae).Cardenas J, Rojas J, Rojas-Fermin L, Lucena M, Buitrago ANat Prod Commun2012Anti-Bacterial Agents/chemistry/*pharmacology, Asteraceae/*chemistry, Bacteria/drug effects, Microbial Sensitivity Tests, Oils, Volatile/chemistry/*pharmacology, Plant Components, Aerial/chemistry, Plant Oils/chemistry/*pharmacology
Genetics23929480Genome Sequence of Klebsiella pneumoniae Strain ATCC 25955, an Oxygen-Insensitive Producer of 1,3-Propanediol.Wang Y, Tao F, Li C, Li L, Xu PGenome Announc10.1128/genomeA.00587-132013

Reference

@idauthorscataloguedoi/urltitle
1826Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4799)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4799
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74446Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3120.1StrainInfo: A central database for resolving microbial strain identifiers