Strain identifier

BacDive ID: 4917

Type strain: No

Species: Escherichia coli

Strain Designation: CF 0034

Strain history: CIP <- 1999, D. Sirot, Fac. Med., Univ. d'Auvergne, Clermont-Ferrand, France: strain CF 0034

NCBI tax ID(s): 562 (species)

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General

@ref: 16273

BacDive-ID: 4917

DSM-Number: 22312

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, antibiotic resistance, human pathogen

description: Escherichia coli CF 0034 is a facultative anaerobe, mesophilic, Gram-negative human pathogen that has multiple antibiotic resistances and was isolated from urinary tract infections.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

@refhistory
16273<- C. Bizet, CIP <- D. Sirot, Fac. Medicine, Univ. d’Auvergne, Clermont-Ferrand, France; CF 0034
118356CIP <- 1999, D. Sirot, Fac. Med., Univ. d'Auvergne, Clermont-Ferrand, France: strain CF 0034

doi: 10.13145/bacdive4917.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 16273

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: CF 0034

type strain: no

Morphology

cell morphology

  • @ref: 118356
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118356

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16273LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
36531MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118356CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
118356CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
16273positivegrowth30mesophilic
36531positivegrowth30mesophilic
118356positivegrowth30-41
118356nogrowth5psychrophilic
118356nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118356
  • oxygen tolerance: facultative anaerobe

antibiogram

  • @ref: 16273
  • medium: Mueller-Hinton Agar
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 0
  • mezlocillin: 10
  • cefalotin: 0
  • cefazolin: 20
  • cefotaxime: 32
  • aztreonam: 30
  • imipenem: 34-36
  • tetracycline: 28
  • chloramphenicol: 0
  • gentamycin: 22
  • amikacin: 22
  • vancomycin: 0
  • erythromycin: 12
  • lincomycin: 0
  • ofloxacin: 40
  • norfloxacin: 40-42
  • colistin: 14
  • pipemidic acid: 32
  • nitrofurantoin: 22
  • bacitracin: 0
  • polymyxin b: 14
  • kanamycin: 10
  • neomycin: 6
  • doxycycline: 22-24
  • ceftriaxone: 32
  • clindamycin: 0
  • fosfomycin: 34
  • moxifloxacin: 34-36
  • linezolid: 0
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 0
  • piperacillin/tazobactam: 20

compound production

@refcompound
16273IRT-3 beta lactamase
16273TEM-32 beta lactamase

observation

  • @ref: 16273
  • observation: ESBL

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
11835629864mannitol+fermentation
11835616947citrate-carbon source
1183564853esculin-hydrolysis
11835617234glucose+fermentation
11835617716lactose+fermentation
11835617632nitrate+reduction
11835616301nitrite+reduction
11835615792malonate-assimilation
118356132112sodium thiosulfate-builds gas from
11835617234glucose+degradation

antibiotic resistance

  • @ref: 118356
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11835635581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole+
11835615688acetoin-
11835617234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
118356oxidase-
118356beta-galactosidase+3.2.1.23
118356alcohol dehydrogenase-1.1.1.1
118356gelatinase-
118356catalase+1.11.1.6
118356lysine decarboxylase+4.1.1.18
118356ornithine decarboxylase+4.1.1.17
118356phenylalanine ammonia-lyase-4.3.1.24
118356tryptophan deaminase-
118356urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118356-+---+----++++-+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
16273++++----+--++-++++-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118356+++++++++++++++-++-+++-++------+-+--+-+----+++-+++----++++-+++------+---+--+---++-++--+-+++++++-+-+

Isolation, sampling and environmental information

isolation

  • @ref: 16273
  • sample type: urinary tract infections
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

Cat1Cat2
#Infection#Disease
#Host Body-Site#Urogenital tract

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
16273yesyes2Risk group (German classification)
1183562Risk group (French classification)

External links

@ref: 16273

culture collection no.: DSM 22312, CIP 105860

straininfo link

  • @ref: 74415
  • straininfo: 68435

Reference

@idauthorscataloguedoi/urltitle
16273Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22312)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22312
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36531Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17926
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
74415Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID68435.1StrainInfo: A central database for resolving microbial strain identifiers
118356Curators of the CIPCollection of Institut Pasteur (CIP 105860)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105860