Strain identifier

BacDive ID: 4900

Type strain: No

Species: Escherichia coli

Strain Designation: E81186

Strain history: CIP <- 2011, CCM <- NCTC <- CPHL, Colindale, UK: strain E81186

NCBI tax ID(s): 1236522 (strain), 562 (species)

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General

@ref: 6743

BacDive-ID: 4900

DSM-Number: 17076

keywords: genome sequence, Bacteria, facultative anaerobe, mesophilic, motile, human pathogen

description: Escherichia coli E81186 is a facultative anaerobe, mesophilic, motile human pathogen that was isolated from clinical specimen.

NCBI tax id

NCBI tax idMatching level
1236522strain
562species

strain history

@refhistory
6743<- NCTC
67770LMG 21756 <-- ATCC 700728 <-- Beckton Dickinson Microbiol. Syst., BDMS T4169 <-- R. Hammann.
118678CIP <- 2011, CCM <- NCTC <- CPHL, Colindale, UK: strain E81186

doi: 10.13145/bacdive4900.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 6743

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: E81186

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.883
6948099.98negative
118678yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6743LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
36445MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118678CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6743positivegrowth37mesophilic
36445positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118678
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.742

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
11867817632nitrate+reduction
11867816301nitrite+reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11867835581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
118678oxidase-
118678catalase+1.11.1.6
118678urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118678-+---++-+-++-+------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
6743+-++----+--++--+-+-+-
6743+-++----+--++--+-+-+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
6743clinical specimenUnited KingdomGBREurope
118678Human, DiarrhoeaAustriaAUTEuropeInnsbruck

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Specimen
#Infection#Medical environment#Clinic

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
6743yesyes2Risk group (German classification)
1186782Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Escherichia coli NCTC12900GCA_900448275contigncbi562
66792Escherichia coli ATCC 700728GCA_000335055contigncbi1051353
66792Escherichia coli JCM 18426GCA_000614425contigncbi1236522
66792Escherichia coli ATCC 7007281051353.3wgspatric1051353
66792Escherichia coli JCM 184261236522.3wgspatric1236522
66792Escherichia coli strain NCTC12900562.34151wgspatric562
66792Escherichia coli JCM 184262585427868draftimg1236522
66792Escherichia coli ATCC 7007282537561605draftimg1051353

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes86.882no
gram-positiveno97.959no
anaerobicno94.91no
aerobicyes84.84no
halophileno90.427no
spore-formingno94.173no
glucose-utilyes94.318no
flagellatedno60.27no
thermophileno99.13no
glucose-fermentyes93.157yes

External links

@ref: 6743

culture collection no.: DSM 17076, NCTC 12900, JCM 18426, ATCC 700728, CCM 4787, CECT 4972, CIP 110281, LMG 21756, LMG 21763, NCIMB 14487, NCIMB 50139, VTT E-011782, WDCM 00014

straininfo link

  • @ref: 74398
  • straininfo: 46252

Reference

@idauthorscataloguedoi/urltitle
6743Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17076)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17076
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36445Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8122
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74398Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46252.1StrainInfo: A central database for resolving microbial strain identifiers
118678Curators of the CIPCollection of Institut Pasteur (CIP 110281)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110281