Strain identifier
BacDive ID: 489
Type strain:
Species: Marinobacter salsuginis
Strain Designation: SD-14B
Strain history: CIP <- 2008, DSMZ <- A.G.L. Antunes, Coimbra Univ., Coimbra, Portugal: strain SD-14B
NCBI tax ID(s): 418719 (species)
General
@ref: 7518
BacDive-ID: 489
DSM-Number: 18347
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Marinobacter salsuginis SD-14B is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from brine–seawater interface.
NCBI tax id
- NCBI tax id: 418719
- Matching level: species
strain history
@ref | history |
---|---|
7518 | <- A. G. L. Antunes, Univ. Coimbra, Portugal; SD-14B <- A. G. L. Antunes and M. Taborda |
122406 | CIP <- 2008, DSMZ <- A.G.L. Antunes, Coimbra Univ., Coimbra, Portugal: strain SD-14B |
doi: 10.13145/bacdive489.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Alteromonadales
- family: Alteromonadaceae
- genus: Marinobacter
- species: Marinobacter salsuginis
- full scientific name: Marinobacter salsuginis Antunes et al. 2007
@ref: 7518
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Alteromonadales
family: Alteromonadaceae
genus: Marinobacter
species: Marinobacter salsuginis
full scientific name: Marinobacter salsuginis Antunes et al. 2007
strain designation: SD-14B
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
32039 | negative | 3 µm | 1 µm | rod-shaped | yes |
122406 | negative | rod-shaped | yes |
pigmentation
- @ref: 32039
- production: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7518 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
36402 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
122406 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7518 | positive | growth | 28 | mesophilic |
32039 | positive | growth | 10-45 | |
32039 | positive | optimum | 36 | mesophilic |
36402 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
32039 | positive | growth | 6.5-9.5 | alkaliphile |
32039 | positive | optimum | 7.75 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
32039 | facultative anaerobe |
122406 | facultative anaerobe |
spore formation
- @ref: 32039
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
32039 | NaCl | positive | growth | 01-20 % |
32039 | NaCl | positive | optimum | 5 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
32039 | 58143 | 5-dehydro-D-gluconate | + | carbon source |
32039 | 30089 | acetate | + | carbon source |
32039 | 16449 | alanine | + | carbon source |
32039 | 28757 | fructose | + | carbon source |
32039 | 5291 | gelatin | + | carbon source |
32039 | 17234 | glucose | + | carbon source |
32039 | 29987 | glutamate | + | carbon source |
32039 | 17754 | glycerol | + | carbon source |
32039 | 24996 | lactate | + | carbon source |
32039 | 25115 | malate | + | carbon source |
32039 | 506227 | N-acetylglucosamine | + | carbon source |
32039 | 28044 | phenylalanine | + | carbon source |
32039 | 26271 | proline | + | carbon source |
32039 | 15361 | pyruvate | + | carbon source |
32039 | 30031 | succinate | + | carbon source |
32039 | 53424 | tween 20 | + | carbon source |
32039 | 53423 | tween 40 | + | carbon source |
32039 | 53426 | tween 80 | + | carbon source |
32039 | 17632 | nitrate | + | reduction |
122406 | 17632 | nitrate | + | reduction |
122406 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 122406
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
32039 | acid phosphatase | + | 3.1.3.2 |
32039 | alkaline phosphatase | + | 3.1.3.1 |
32039 | catalase | + | 1.11.1.6 |
32039 | gelatinase | + | |
32039 | cytochrome oxidase | + | 1.9.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
122406 | oxidase | + | |
122406 | catalase | + | 1.11.1.6 |
122406 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122406 | - | + | - | + | + | + | + | - | - | - | + | + | - | - | - | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
7518 | brine–seawater interface | Shaban Deep, Red Sea | Saudi Arabia | SAU | Asia |
122406 | Environment, Brine-seawater interface | Shaban Deep, Red Sea | Saudi Arabia | SAU | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_14463.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_1317;97_1564;98_10960;99_14463&stattab=map
- Last taxonomy: Marinobacter salsuginis subclade
- 16S sequence: EF028328
- Sequence Identity:
- Total samples: 1044
- soil counts: 42
- aquatic counts: 943
- animal counts: 50
- plant counts: 9
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
7518 | 1 | Risk group (German classification) |
122406 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 7518
- description: Marinobacter salsuginis strain SD-14B 16S ribosomal RNA gene, partial sequence
- accession: EF028328
- length: 1487
- database: ena
- NCBI tax ID: 1260625
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Marinobacter salsuginis SD-14B | GCA_004936695 | contig | ncbi | 1260625 |
66792 | Marinobacter salsuginis SD-14B | 1260625.5 | wgs | patric | 1260625 |
66792 | Marinobacter salsuginis SD-14B | 2516653033 | draft | img | 1260625 |
GC content
- @ref: 7518
- GC-content: 55.9
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 92.503 | yes |
gram-positive | no | 98.675 | yes |
anaerobic | no | 98.23 | yes |
aerobic | yes | 78.228 | yes |
halophile | yes | 85.086 | no |
spore-forming | no | 96.363 | yes |
thermophile | no | 98.716 | no |
glucose-util | yes | 51.792 | no |
flagellated | yes | 89.266 | no |
glucose-ferment | no | 90.121 | no |
External links
@ref: 7518
culture collection no.: DSM 18347, CIP 109893, LMG 23697
straininfo link
- @ref: 70168
- straininfo: 292702
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17473254 | Marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the Shaban Deep, Red Sea. | Antunes A, Franca L, Rainey FA, Huber R, Nobre MF, Edwards KJ, da Costa MS | Int J Syst Evol Microbiol | 10.1099/ijs.0.64862-0 | 2007 | Anaerobiosis, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Flagella, Genes, rRNA, Hydrogen-Ion Concentration, Indian Ocean, Marinobacter/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Movement, Nitrates/metabolism, Nitrogen/metabolism, Oxidation-Reduction, Phylogeny, Pigments, Biological/biosynthesis, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Temperature | Metabolism |
Phylogeny | 27902222 | Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment. | Rani S, Koh HW, Kim H, Rhee SK, Park SJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001587 | 2017 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Marinobacter/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry | Transcriptome |
Phylogeny | 27902258 | Marinobacter halotolerans sp. nov., a halophilic bacterium isolated from a saltern crystallizing pond. | Kim JO, Lee HJ, Han SI, Whang KS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001653 | 2017 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Marinobacter/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Salinity, Sequence Analysis, DNA, Ubiquinone/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7518 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18347) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18347 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
32039 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28289 | 28776041 | |
36402 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7688 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
70168 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID292702.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
122406 | Curators of the CIP | Collection of Institut Pasteur (CIP 109893) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109893 |