Strain identifier
BacDive ID: 4698
Type strain: 
Species: Escherichia coli
Strain Designation: EW227
Strain history: <- L. Beutin, NRZEC of the BGA, Berlin
NCBI tax ID(s): 562 (species)
version 8.1 (current version)
General
@ref: 3389
BacDive-ID: 4698
DSM-Number: 9031
keywords: Bacteria, mesophilic, human pathogen
description: Escherichia coli EW227 is a mesophilic human pathogen that was isolated from Human diarrhoea.
NCBI tax id
- NCBI tax id: 562
- Matching level: species
strain history
- @ref: 3389
- history: <- L. Beutin, NRZEC of the BGA, Berlin
doi: 10.13145/bacdive4698.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Escherichia
- species: Escherichia coli
- full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus coli
@ref: 3389
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Escherichia
species: Escherichia coli
full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919
strain designation: EW227
type strain: no
Culture and growth conditions
culture medium
- @ref: 3389
- name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
- growth: yes
- link: https://mediadive.dsmz.de/medium/381
- composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
culture temp
- @ref: 3389
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 30849 | L-arabinose | + | fermentation |
| 68368 | 27613 | amygdalin | - | fermentation |
| 68368 | 28053 | melibiose | + | fermentation |
| 68368 | 17992 | sucrose | - | fermentation |
| 68368 | 62345 | L-rhamnose | - | fermentation |
| 68368 | 30911 | sorbitol | + | fermentation |
| 68368 | 17268 | myo-inositol | - | fermentation |
| 68368 | 16899 | D-mannitol | + | fermentation |
| 68368 | 17634 | D-glucose | + | fermentation |
| 68368 | 5291 | gelatin | - | hydrolysis |
| 68368 | 27897 | tryptophan | + | energy source |
| 68368 | 16199 | urea | - | hydrolysis |
| 68368 | 16947 | citrate | - | assimilation |
| 68368 | 18257 | ornithine | - | degradation |
| 68368 | 25094 | lysine | - | degradation |
| 68368 | 29016 | arginine | - | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 15688 | acetoin | no |
| 68368 | 35581 | indole | yes |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | - | |
| 68368 | 35581 | indole | + |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | cytochrome oxidase | - | 1.9.3.1 |
| 68368 | gelatinase | - | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | - | 3.5.1.5 |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 |
| 68368 | lysine decarboxylase | - | 4.1.1.18 |
| 68368 | arginine dihydrolase | - | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3389 | + | - | - | - | - | - | - | - | + | - | - | + | + | - | + | - | - | + | - | + | |
| 3389 | + | - | - | - | - | - | - | - | + | - | - | + | + | - | + | - | - | + | - | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 3389
- sample type: Human diarrhoea
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 |
|---|---|
| #Infection | #Disease |
| #Host Body-Site | #Gastrointestinal tract |
| #Host | #Human |
Safety information
risk assessment
- @ref: 3389
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 3389
culture collection no.: DSM 9031
straininfo link
- @ref: 74194
- straininfo: 48570
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3389 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9031) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9031 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68368 | Automatically annotated from API 20E | |||
| 74194 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48570.1 | StrainInfo: A central database for resolving microbial strain identifiers |