Strain identifier

BacDive ID: 459

Type strain: No

Species: Marinobacterium iners

Strain Designation: KW-40

Strain history: CIP <- 1997, J. Gonzales, Georgia Univ., Athens, GA, USA, Marinobacterium georgiense: strain KW-40

NCBI tax ID(s): 48076 (species)

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General

@ref: 4401

BacDive-ID: 459

DSM-Number: 11526

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Marinobacterium iners KW-40 is a facultative aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from enrichment community growing on the high molecular weight fraction of a black liquor sample from the Federal Paper Board Company Inc..

NCBI tax id

  • NCBI tax id: 48076
  • Matching level: species

strain history

@refhistory
4401<- J. M. González, KW-40
67770IAM 15212 <-- CIP 105236 <-- J. Gonzales KW-40.
120100CIP <- 1997, J. Gonzales, Georgia Univ., Athens, GA, USA, Marinobacterium georgiense: strain KW-40

doi: 10.13145/bacdive459.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacterium
  • species: Marinobacterium iners
  • full scientific name: Marinobacterium iners (Iizuka and Komagata 1964) Tindall 2020

  • synonyms

    @refsynonym
    20215Pseudomonas iners
    20215Marinobacterium georgiense

@ref: 4401

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacterium

species: Marinobacterium iners

full scientific name: Marinobacterium iners (Iizuka and Komagata 1964) Tindall 2020

strain designation: KW-40

type strain: no

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23292negative1.6-2.3 µm0.5-0.7 µmrod-shapedyesmonotrichous, polar
69480yes96.74
69480negative99.999
120100negativerod-shapedyes

colony morphology

  • @ref: 23292
  • colony size: 0.5 mm
  • colony color: translucent
  • colony shape: circular
  • incubation period: 7 days
  • medium used: Marine agar 2216

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4401BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23292Marine agar (MA)yes
35796Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120100CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
4401positivegrowth28-37mesophilic
23292positivegrowth4-41
23292positiveoptimum37mesophilic
35796positivegrowth30mesophilic
67770positivegrowth30mesophilic
120100positivegrowth30-41
120100nogrowth5psychrophilic
120100nogrowth10psychrophilic

culture pH

@refabilitytypepHPH range
23292positiveoptimum7.5
23292positivegrowth5.5-9.5alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23292facultative aerobe
120100obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.994

halophily

@refsaltgrowthtested relationconcentration
23292NaClpositivegrowth0.1-2 M
23292NaClpositiveoptimum0.1-0.5 M
120100NaClpositivegrowth2-6 %
120100NaClnogrowth0 %
120100NaClnogrowth8 %
120100NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2329217234glucose-builds acid from
2329216716benzene-growth
2329215428glycine-growth
2329216467L-arginine-growth
2329215971L-histidine-growth
2329217191L-isoleucine-growth
2329215603L-leucine-growth
2329216643L-methionine-growth
2329217115L-serine-growth
2329216857L-threonine-growth
2329216828L-tryptophan-growth
2329216414L-valine-growth
2329217716lactose-growth
23292506227N-acetylglucosamine-growth
2329226546rhamnose-growth
2329230762salicylate-growth
2329217992sucrose-growth
232922509agar-hydrolysis
2329217029chitin-hydrolysis
232925291gelatin-hydrolysis
2329228017starch-hydrolysis
2329217632nitrate-reduction
23292323734-coumarate+growth
23292178794-hydroxybenzoate+growth
2329230089acetate+growth
2329216150benzoate+growth
2329228885butanol+growth
2329217968butyrate+growth
2329217057cellobiose+growth
2329223248cinnamate+growth
2329216947citrate+growth
23292370543-hydroxybutyrate+growth
2329216236ethanol+growth
2329229749ferulate+growth
2329215740formate+growth
2329228757fructose+growth
2329217234glucose+growth
2329217754glycerol+growth
2329216977L-alanine+growth
2329217196L-asparagine+growth
2329229991L-aspartate+growth
2329229985L-glutamate+growth
2329218019L-lysine+growth
2329217295L-phenylalanine+growth
2329217203L-proline+growth
2329225115malate+growth
2329237684mannose+growth
2329217790methanol+growth
2329216830methylamine+growth
2329215882phenol+growth
2329228831propanol+growth
23292178242-propanol+growth
2329217272propionate+growth
2329215361pyruvate+growth
2329230031succinate+growth
2329216632vanillate+growth
2329218222xylose+growth
2329253426tween 80+hydrolysis
1201004853esculin-hydrolysis
12010017632nitrate-reduction
12010016301nitrite-reduction
12010017632nitrate-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2329218208penicillin gyesyes
2329228001vancomycinyesyes
2329228971ampicillinyesyes
2329217698chloramphenicolyesyes
232926104kanamycinyesyes
2329217076streptomycinyesyes
2329227902tetracyclineyesyes

metabolite production

@refChebi-IDmetaboliteproduction
2329253388polyhydroxybutyrateno
12010035581indoleno

enzymes

@refvalueactivityec
23292catalase+1.11.1.6
23292cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120100oxidase+
120100beta-galactosidase-3.2.1.23
120100alcohol dehydrogenase-1.1.1.1
120100gelatinase-
120100amylase-
120100caseinase-3.4.21.50
120100catalase+1.11.1.6
120100lecithinase-
120100lipase-
120100lysine decarboxylase-4.1.1.18
120100ornithine decarboxylase-4.1.1.17
120100tryptophan deaminase-
120100urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120100-++--+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120100-------------------------------------------------+------------+---+------------++-------+----+--+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureisolation date
4401enrichment community growing on the high molecular weight fraction of a black liquor sample from the Federal Paper Board Company Inc.Georgia, AugustaUSAUSANorth America
23292salt marsh on the coastmarine enrichment community growing on pulp mill eflluent as the sole carbon source
67770Costal seawaterGAUSAUSANorth America
120100Environment, Coastal seawaterGeorgiaUnited States of AmericaUSANorth America1994

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Industrial production
#Engineered#Laboratory#Lab enrichment
#Environmental#Microbial community

taxonmaps

  • @ref: 69479
  • File name: preview.99_83980.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_240;96_6773;97_8107;98_57388;99_83980&stattab=map
  • Last taxonomy: Marinobacterium georgiense subclade
  • 16S sequence: U58339
  • Sequence Identity:
  • Total samples: 151
  • soil counts: 10
  • aquatic counts: 124
  • animal counts: 17

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
44011Risk group (German classification)
1201001Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4401Marinobacterium georgiense 16S ribosomal RNA gene, partial sequenceU583391435ena48076
67770Marinobacterium georgiense gene for 16S rRNA, partial sequence, strain: NBRC 102606AB6818811468ena48076

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinobacterium georgiense DSM 115261122198.3wgspatric1122198
66792Marinobacterium georgiense DSM 115262634166328draftimg1122198
67770Marinobacterium iners DSM 11526GCA_900107855scaffoldncbi1122198

GC content

  • @ref: 23292
  • GC-content: 54.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes93.988yes
flagellatedyes94.616no
gram-positiveno99.407yes
anaerobicno94.06yes
aerobicyes58.472no
halophileyes73.565no
spore-formingno93.93no
thermophileno99.172yes
glucose-utilyes58.316yes
glucose-fermentno86.751no

External links

@ref: 4401

culture collection no.: DSM 11526, ATCC 700074, JCM 21667, CIP 105236, IAM 15212, NBRC 102606, CECT 7200

straininfo link

  • @ref: 70138
  • straininfo: 43757

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9103623Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community.Gonzalez JM, Mayer F, Moran MA, Hodson RE, Whitman WBInt J Syst Bacteriol10.1099/00207713-47-2-3691997Base Composition, Base Sequence, Biodegradation, Environmental, DNA Primers/genetics, DNA, Bacterial/chemistry/genetics, Fatty Acids/analysis, Gram-Negative Aerobic Bacteria/classification/genetics/*metabolism, Lignin/*metabolism, Marine Biology, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic, Water MicrobiologyMetabolism
Phylogeny17220446Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea.Chang HW, Nam YD, Kwon HY, Park JR, Lee JS, Yoon JH, An KG, Bae JWInt J Syst Evol Microbiol10.1099/ijs.0.64505-02007Alteromonadaceae/*classification/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNAGenetics
Phylogeny19643903Marinobacterium maritimum sp. nov., a marine bacterium isolated from Arctic sediment.Kim SJ, Park SJ, Yoon DN, Park BJ, Choi BR, Lee DH, Roh Y, Rhee SKInt J Syst Evol Microbiol10.1099/ijs.0.009134-02009Alteromonadaceae/*classification/genetics/*isolation & purification/metabolism, Arctic Regions, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4401Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11526)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11526
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23292J. M. GONZáLEZ, F. MAYER, M. A. MORAN, R. E. HODSON, W. B. WHITMAN10.1099/00207713-47-2-369Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., Two Marine Bacteria from a Lignin-Rich Pulp Mill Waste Enrichment CommunityIJSEM 47: 369-376 19979103623
35796Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17233
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70138Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43757.1StrainInfo: A central database for resolving microbial strain identifiers
120100Curators of the CIPCollection of Institut Pasteur (CIP 105236)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105236