Strain identifier

BacDive ID: 4586

Type strain: No

Species: Escherichia coli

Strain Designation: F.S. Barr 1532, 1532

Strain history: CIP <- 1986, R. Sutherland, Beecham Pharmaceuticals, UK: strain 1532

NCBI tax ID(s): 562 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2367

BacDive-ID: 4586

DSM-Number: 5923

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, antibiotic resistance, human pathogen

description: Escherichia coli F.S. Barr 1532 is an aerobe, mesophilic, Gram-negative human pathogen that has multiple antibiotic resistances and was isolated from Canine.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

@refhistory
2367<- ATCC
420921986, R. Sutherland, Beecham Pharmaceuticals, UK: strain 1532
118686CIP <- 1986, R. Sutherland, Beecham Pharmaceuticals, UK: strain 1532

doi: 10.13145/bacdive4586.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 2367

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: F.S. Barr 1532, 1532

type strain: no

Morphology

cell morphology

  • @ref: 118686
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118686
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2367LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
42092MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118686CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
2367positivegrowth37mesophilic
42092positivegrowth30mesophilic
50172positivegrowth30mesophilic
118686positivegrowth10-41
118686nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50172aerobe
118686facultative anaerobe

antibiogram

@refmediumPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
2367Mueller-Hinton Agar00601418-202436343226-2882222010-1203434142822-240142020223403632000026
2367Mueller-Hinton Agar006014-16202640403428-30822-2422-24012036381430240163220-22223603830-32000028

compound production

  • @ref: 2367
  • compound: ß lactamase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
11868629864mannitol+fermentation
11868616947citrate-carbon source
1186864853esculin+hydrolysis
11868617234glucose+fermentation
11868617716lactose+fermentation
11868617632nitrate+reduction
11868616301nitrite+reduction
11868615792malonate-assimilation
118686132112sodium thiosulfate-builds gas from
11868617234glucose+degradation
6837418257ornithine+degradation
6837429016arginine-hydrolysis
6837425094lysine+degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid+builds acid from
68374Potassium 5-ketogluconate+builds acid from
6837416899D-mannitol+builds acid from
6837417306maltose+builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan+energy source
6837417634D-glucose+builds acid from
6837417992sucrose+builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose+builds acid from
6837462345L-rhamnose+builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

antibiotic resistance

  • @ref: 118686
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11868635581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837435581indole+
6836815688acetoin-
6836835581indole+
11868615688acetoin-
11868617234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase+3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase+4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
118686oxidase-
118686beta-galactosidase+3.2.1.23
118686alcohol dehydrogenase-1.1.1.1
118686gelatinase-
118686catalase+1.11.1.6
118686lysine decarboxylase+4.1.1.18
118686ornithine decarboxylase+4.1.1.17
118686phenylalanine ammonia-lyase+4.3.1.24
118686tryptophan deaminase-
118686urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    50172C12:06.312
    50172C14:011.214
    50172C15:01.515
    50172C16:026.616
    50172C14:0 3OH/C16:1 ISO I12.815.485
    50172C16:1 ω7c1015.819
    50172C17:0 CYCLO16.216.888
    50172C18:1 ω7c /12t/9t12.617.824
    50172C19:0 CYCLO ω8c2.118.9
    50172unknown 14.5030.814.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118686-+---+----++-+-+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
2367+-++----+--++-++++--
2367+-++----+--++-++++--
2367+-++----+--++-++++---
50172+-++----+--++-++++---

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
50172+-+--++-+-++--+-+-+++---+++-----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118686+++++++++++++++---++++-++----+++-+--+-++---+++---+---+++-+-++------------+-+---++-+---+--++++++-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
50172CanineTennesseeUSAUSANorth America
118686Animal, Canine

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Canidae (Dog)

taxonmaps

  • @ref: 69479
  • File name: preview.99_8.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_7;99_8&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: AM980865
  • Sequence Identity:
  • Total samples: 97630
  • soil counts: 2111
  • aquatic counts: 5613
  • animal counts: 88071
  • plant counts: 1835

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
2367yesyes2Risk group (German classification)
1186862Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Escherichia coli partial 16S rRNA gene, strain ATCC 35218AM9808651509ena562
20218Escherichia coli 16S-23S ribosomal RNA intergenic spacer, partial sequenceEF436579436ena562

External links

@ref: 2367

culture collection no.: DSM 5923, ATCC 35218, DSM 5564, CCUG 30600, CIP 102181, NCTC 11954

straininfo link

  • @ref: 74079
  • straininfo: 92279

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2367Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5923)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5923
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42092Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13839
50172Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30600)https://www.ccug.se/strain?id=30600
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74079Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92279.1StrainInfo: A central database for resolving microbial strain identifiers
118686Curators of the CIPCollection of Institut Pasteur (CIP 102181)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102181