Strain identifier
BacDive ID: 4474
Type strain:
Species: Escherichia coli
Strain Designation: K12 JM83, JM83
Strain history: <- H. Bahl, Univ. Göttingen <- W. Gruissem, Univ. Berkeley
NCBI tax ID(s): 562 (species)
General
@ref: 1526
BacDive-ID: 4474
DSM-Number: 3947
keywords: Bacteria, mesophilic
description: Escherichia coli K12 JM83 is a mesophilic bacterium of the family Enterobacteriaceae.
NCBI tax id
- NCBI tax id: 562
- Matching level: species
strain history
- @ref: 1526
- history: <- H. Bahl, Univ. Göttingen <- W. Gruissem, Univ. Berkeley
doi: 10.13145/bacdive4474.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Escherichia
- species: Escherichia coli
- full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus coli
@ref: 1526
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Escherichia
species: Escherichia coli
full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919
strain designation: K12 JM83, JM83
type strain: no
Culture and growth conditions
culture medium
- @ref: 1526
- name: MINERAL MEDIUM M9 for E. coli JM strains (DSMZ Medium 382)
- growth: yes
- link: https://mediadive.dsmz.de/medium/382
- composition: Name: MINERAL MEDIUM M9 for E. coli JM strains (DSMZ Medium 382) Composition: Na2HPO4 6.0 g/l KH2PO4 3.0 g/l Glucose 2.0 g/l NH4Cl 1.0 g/l NaCl 0.5 g/l Thiamine-HCl x 2 H2O 0.373301 g/l MgSO4 0.12037 g/l Proline 0.02 g/l CaCl2 0.011098 g/l Distilled water
culture temp
- @ref: 1526
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | + | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | + | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | + | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | + | builds acid from | 17924 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
68368 | L-arabinose | - | fermentation | 30849 |
68368 | amygdalin | - | fermentation | 27613 |
68368 | sucrose | - | fermentation | 17992 |
68368 | L-rhamnose | + | fermentation | 62345 |
68368 | sorbitol | + | fermentation | 30911 |
68368 | myo-inositol | - | fermentation | 17268 |
68368 | D-mannitol | - | fermentation | 16899 |
68368 | D-glucose | + | fermentation | 17634 |
68368 | gelatin | - | hydrolysis | 5291 |
68368 | tryptophan | + | energy source | 27897 |
68368 | urea | - | hydrolysis | 16199 |
68368 | citrate | - | assimilation | 16947 |
68368 | ornithine | - | degradation | 18257 |
68368 | lysine | + | degradation | 25094 |
68368 | arginine | - | hydrolysis | 29016 |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | yes |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1526 | - | - | + | - | - | - | - | - | + | - | - | + | - | - | + | + | - | - | - | - | |
1526 | - | - | + | - | - | - | - | - | + | - | - | + | - | - | + | + | - | + | - | - | |
1526 | - | - | + | - | - | - | - | - | + | - | - | + | - | - | + | + | - | + | - | - | |
1526 | - | - | + | - | - | - | - | - | + | - | - | + | - | - | + | + | - | + | - | - | - |
1526 | - | - | + | - | - | - | - | - | + | - | - | + | - | - | + | + | - | + | - | - | - |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1526 | - | + | - | - | - | + | - | - | - | - | + | + | + | + | - | - | - | - | +/- | + | - | - | + | - | - | - | - | - | + | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | + |
1526 | - | + | - | - | - | + | - | - | - | - | + | + | + | + | - | + | - | - | +/- | + | - | - | + | - | - | - | - | - | + | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | + |
Safety information
risk assessment
- @ref: 1526
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 1526
culture collection no.: DSM 3947
straininfo link
- @ref: 73965
- straininfo: 47782
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
1526 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3947) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3947 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
68368 | Automatically annotated from API 20E | |||
68371 | Automatically annotated from API 50CH acid | |||
73965 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47782.1 | StrainInfo: A central database for resolving microbial strain identifiers |