Strain identifier

BacDive ID: 447

Type strain: Yes

Species: Alteromonas stellipolaris

Strain Designation: ANT69a, R-15466

Strain history: CIP <- 2004, S. Van Trappen, Gent Univ., Gent, Belgium: strain ANT 69a <- J. Mergaert, Gent Univ., Belgium <- T. L. Tan, Bremenhaven, Germany

NCBI tax ID(s): 233316 (species)

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General

@ref: 6058

BacDive-ID: 447

DSM-Number: 15691

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-negative, motile, rod-shaped, colony-forming, pigmented

description: Alteromonas stellipolaris ANT69a is an obligate aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and has a brown pigmentation.

NCBI tax id

  • NCBI tax id: 233316
  • Matching level: species

strain history

@refhistory
6058<- S. Van Trappen, Univ. Ghent, Belgium; R-15466 <- T.-L. Tan, AWI; ANT69a
123306CIP <- 2004, S. Van Trappen, Gent Univ., Gent, Belgium: strain ANT 69a <- J. Mergaert, Gent Univ., Belgium <- T. L. Tan, Bremenhaven, Germany

doi: 10.13145/bacdive447.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Alteromonas
  • species: Alteromonas stellipolaris
  • full scientific name: Alteromonas stellipolaris Van Trappen et al. 2004

  • synonyms

    • @ref: 20215
    • synonym: Alteromonas addita

@ref: 6058

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Alteromonas

species: Alteromonas stellipolaris

full scientific name: Alteromonas stellipolaris Van Trappen et al. 2004

strain designation: ANT69a, R-15466

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23288negative2.0-7.0 µm0.4 µmrod-shapedyesmonotrichous, polar
69480yes98.47
69480negative99.994
123306negativerod-shapedno

colony morphology

  • @ref: 23288
  • colony size: 2-5 mm
  • colony color: creamy white, opaque
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Marine agar

pigmentation

  • @ref: 23288
  • production: yes
  • color: brown

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6058BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23288Marine agar (MA)yes
23288PYG agaryes
33972Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
123306CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6058positivegrowth20psychrophilic
23288positivegrowth5-37
23288nogrowth>40thermophilic
33972positivegrowth25mesophilic
123306positivegrowth5-30
123306nogrowth37mesophilic
123306nogrowth41thermophilic

culture pH

@refabilitytypepHPH range
23288positivegrowth6-9alkaliphile
23288positivegrowth7.0-8.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23288obligate aerobe
123306facultative anaerobe

spore formation

@refspore formationconfidence
23288no
69481no100
69480no99.973

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23288NaClpositivegrowth1-10 %
23288halophilic
123306NaClpositivegrowth4-8 %
123306NaClnogrowth0 %
123306NaClnogrowth2 %
123306NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2328817521(-)-quinic acid-assimilation
23288309162-oxoglutarate-assimilation
23288286442-oxopentanoate-assimilation
23288620642,3-butanediol-assimilation
23288182404-hydroxy-L-proline-assimilation
23288181014-hydroxyphenylacetic acid-assimilation
2328815963ribitol-assimilation
23288645522-hydroxybutyrate-assimilation
2328873706bromosuccinate-assimilation
2328816383cis-aconitate-assimilation
2328816947citrate-assimilation
2328815570D-alanine-assimilation
2328818333D-arabitol-assimilation
2328812931D-galactonate-assimilation
2328817784D-glucosaminic acid-assimilation
2328814314D-glucose 6-phosphate-assimilation
2328815748D-glucuronate-assimilation
2328816523D-serine-assimilation
2328817924D-sorbitol-assimilation
2328817126DL-carnitine-assimilation
2328817113erythritol-assimilation
2328816000ethanolamine-assimilation
2328815740formate-assimilation
2328816865gamma-aminobutyric acid-assimilation
23288167244-hydroxybutyrate-assimilation
2328829042glucose 1-phosphate-assimilation
2328832323glucuronamide-assimilation
2328814336glycerol 1-phosphate-assimilation
2328873804glycyl L-aspartic acid-assimilation
2328817240itaconate-assimilation
2328830849L-arabinose-assimilation
2328817196L-asparagine-assimilation
2328829991L-aspartate-assimilation
2328818287L-fucose-assimilation
2328815971L-histidine-assimilation
2328815729L-ornithine-assimilation
2328817295L-phenylalanine-assimilation
2328818183L-pyroglutamic acid-assimilation
2328862345L-rhamnose-assimilation
2328824996lactate-assimilation
2328825000lactone-assimilation
2328815792malonate-assimilation
2328817268myo-inositol-assimilation
2328851850methyl pyruvate-assimilation
2328875146monomethyl succinate-assimilation
2328828037N-acetylgalactosamine-assimilation
23288506227N-acetylglucosamine-assimilation
2328850048phenylethylamine-assimilation
2328817148putrescine-assimilation
2328841865sebacic acid-assimilation
23288143136succinamate-assimilation
2328817748thymidine-assimilation
2328827248urocanic acid-assimilation
2328817151xylitol-assimilation
2328822599arabinose-builds acid from
2328817234glucose-builds acid from
2328817268myo-inositol-builds acid from
2328826546rhamnose-builds acid from
2328830911sorbitol-builds acid from
2328816947citrate-carbon source
2328858187alginate-degradation
2328817029chitin-degradation
2328816199urea-hydrolysis
2328817632nitrate-reduction
2328830089acetate+assimilation
232888295beta-hydroxybutyrate+assimilation
2328817057cellobiose+assimilation
2328815824D-fructose+assimilation
2328812936D-galactose+assimilation
2328818024D-galacturonic acid+assimilation
2328817634D-glucose+assimilation
2328817716lactose+assimilation
2328816899D-mannitol+assimilation
2328816024D-mannose+assimilation
2328828053melibiose+assimilation
2328827605D-psicose+assimilation
2328816634raffinose+assimilation
2328816551D-trehalose+assimilation
2328823652dextrin+assimilation
23288egg yolk+assimilation
2328828066gentiobiose+assimilation
2328828087glycogen+assimilation
2328821217L-alaninamide+assimilation
2328873786L-alanylglycine+assimilation
2328829985L-glutamate+assimilation
232886359lactulose+assimilation
2328817306maltose+assimilation
2328873784glycyl-l-glutamate+assimilation
2328817272propionate+assimilation
2328817992sucrose+assimilation
2328853423tween 40+assimilation
2328853426tween 80+assimilation
2328827613amygdalin+builds acid from
2328816991dna+degradation
232884853esculin+degradation
232885291gelatin+degradation
2328828017starch+degradation
23288167632-oxobutanoate+/-assimilation
2328823456cyclodextrin+/-assimilation
232888391D-gluconate+/-assimilation
2328817754glycerol+/-assimilation
2328817596inosine+/-assimilation
2328816977L-alanine+/-assimilation
2328815603L-leucine+/-assimilation
2328817203L-proline+/-assimilation
2328816857L-threonine+/-assimilation
2328830031succinate+/-assimilation
2328816704uridine+/-assimilation
2328829864mannitol+/-builds acid from
2328828053melibiose+/-builds acid from
2328817992sucrose+/-builds acid from
1233064853esculin+hydrolysis
12330617632nitrate-reduction
12330616301nitrite-reduction
123306132112sodium thiosulfate-builds gas from

metabolite production

@refChebi-IDmetaboliteproduction
2328853388polyhydroxybutyrateno
2328835581indoleno
2328816136hydrogen sulfideno
2328815688acetoinno
12330635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testcitrate test
2328815688acetoin-
2328816947citrate-

enzymes

@refvalueactivityec
23288acid phosphatase+3.1.3.2
23288alkaline phosphatase+3.1.3.1
23288alpha-chymotrypsin-3.4.21.1
23288alpha-fucosidase-3.2.1.51
23288alpha-galactosidase+/-3.2.1.22
23288alpha-glucosidase+3.2.1.20
23288arginine dihydrolase-3.5.3.6
23288beta-galactosidase+3.2.1.23
23288beta-glucuronidase-3.2.1.31
23288catalase+1.11.1.6
23288cystine arylamidase-3.4.11.3
23288cytochrome oxidase+1.9.3.1
23288esterase (C 4)+/-
23288esterase lipase (C 8)+/-
23288leucine arylamidase+3.4.11.1
23288lipase (C 14)+/-
23288lysine decarboxylase-4.1.1.18
23288naphthol-AS-BI-phosphohydrolase+
23288ornithine decarboxylase-4.1.1.17
23288trypsin+/-3.4.21.4
23288tryptophan deaminase-4.1.99.1
23288valine arylamidase+/-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123306oxidase+
123306beta-galactosidase+3.2.1.23
123306alcohol dehydrogenase-1.1.1.1
123306gelatinase+
123306amylase+
123306DNase+
123306caseinase+3.4.21.50
123306catalase+1.11.1.6
123306tween esterase+
123306lecithinase+
123306lipase+
123306lysine decarboxylase-4.1.1.18
123306ornithine decarboxylase-4.1.1.17
123306protease+
123306tryptophan deaminase-
123306urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123306-++++++---++-+-++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentlatitudelongitudecountryisolation date
6058seawater66°20.0'S, 08°53.4'EAustralia and Oceania-66.33338.89
123306Environment, Sea waterAntarctic Ocean1990

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_700.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_98;96_261;97_285;98_318;99_700&stattab=map
  • Last taxonomy: Alteromonas
  • 16S sequence: AJ295715
  • Sequence Identity:
  • Total samples: 655
  • aquatic counts: 653
  • animal counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
60581Risk group (German classification)
1233061Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
23288Alteromonas stellipolaris partial 16S rRNA gene, type strain LMG 21861TAJ2957151485nuccore233316
6058Isolepis meruensis chloroplast trnL gene intronAJ295791669ena130952

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alteromonas stellipolaris strain LMG 21861233316.13plasmidpatric233316
66792Alteromonas stellipolaris strain LMG 21861233316.3completepatric233316
66792Alteromonas stellipolaris LMG 218612687453500completeimg233316

GC content

  • @ref: 23288
  • GC-content: 43.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes91.062yes
gram-positiveno99.058no
anaerobicno98.508yes
aerobicyes91.243yes
halophileyes75.881no
spore-formingno96.362no
glucose-utilyes90.29yes
flagellatedyes90.405no
thermophileno99.581no
glucose-fermentno86.401no

External links

@ref: 6058

culture collection no.: DSM 15691, LMG 21861, CIP 108513, ANT 69a

straininfo link

  • @ref: 70126
  • straininfo: 28805

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15280285Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas.Van Trappen S, Tan TL, Yang J, Mergaert J, Swings JInt J Syst Evol Microbiol10.1099/ijs.0.02862-02004Adaptation, Biological, Alteromonas/*classification/cytology/*isolation & purification/physiology, Antarctic Regions, Cold Temperature, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Osmotic Pressure, Phenazines, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sequence Homology, Water MicrobiologyEnzymology
Phylogeny23221523Isolation and characterization of an agarase-producing bacterial strain, Alteromonas sp. GNUM-1, from the West Sea, Korea.Kim J, Hong SKJ Microbiol Biotechnol10.4014/jmb.1209.080872012Agar/metabolism, Alteromonas/chemistry/*enzymology/genetics/*isolation & purification, Cell Count, Culture Media, Glycoside Hydrolases/*biosynthesis/genetics/isolation & purification/metabolism, Hydrogen-Ion Concentration, Korea, Molecular Sequence Data, Phenotype, Phylogeny, Sargassum/microbiology, Sodium Chloride, Water MicrobiologyEnzymology
Phylogeny30362935Salinimonas sediminis sp. nov., a piezophilic bacterium isolated from a deep-sea sediment sample from the New Britain Trench.Cao J, Lai Q, Liu P, Wei Y, Wang L, Liu R, Fang JInt J Syst Evol Microbiol10.1099/ijsem.0.0030552018Alteromonadaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cold Temperature, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Geologic Sediments/*microbiology, Pacific Ocean, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6058Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15691)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15691
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23288Stefanie Van Trappen, Tjhing-Lok Tan, Jifang Yang, Joris Mergaert, Jean Swings10.1099/ijs.0.02862-0Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus AlteromonasIJSEM 54: 1157-1163 200415280285
33972Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6144
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70126Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID28805.1StrainInfo: A central database for resolving microbial strain identifiers
123306Curators of the CIPCollection of Institut Pasteur (CIP 108513)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108513