Strain identifier

BacDive ID: 4420

Type strain: No

Species: Escherichia coli

Strain history: 1954, Centre de la Pénicilline, Paris, France: strain MacLeod

NCBI tax ID(s): 562 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 358

BacDive-ID: 4420

DSM-Number: 682

keywords: antibiotic resistance, mesophilic, aerobe, Bacteria, genome sequence, human pathogen

description: Escherichia coli CCUG 3274 is an aerobe, mesophilic human pathogen that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

doi: 10.13145/bacdive4420.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 358

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
358NUTRIENT AGAR (DSMZ Medium 1)yeshttps://bacmedia.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39734MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
358positivegrowth37mesophilic
39734positivegrowth30mesophilic
44629positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44629
  • oxygen tolerance: aerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
358Mueller-Hinton Agar371aerob802432-342820-222838383428-3032-342424-2601003638142824016242026361036341000026
358Mueller-Hinton Agar371aerob10022322622283636322830242601003638142822-240142420-2224-2636-38028-3036800026-28
358Mueller-Hinton Agar37aerob10-12024342822283836-383830-32342828010-12036-3840142824-26016262428-303803636-388-1080028
358Mueller-Hinton Agar802430-3226222836-3836-38322830-32262801003636-3814-162824-26014-16362226-283803636800026-28
358Mueller-Hinton Agar80223228222838383426-2830-32222608-10034-363814282601424242438-4003436000028
358Mueller Hinton37aerob60223430263040403628-30342424012040421630-32260182420284003440000030

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837430911sorbitol-builds acid from
6837417057cellobiose-builds acid from
6837417268myo-inositol-builds acid from
6837462345L-rhamnose-builds acid from
6837427082trehalose+builds acid from
6837418333D-arabitol-builds acid from
6837430849L-arabinose+builds acid from
6837417992sucrose-builds acid from
6837417634D-glucose-builds acid from
6837427897tryptophan+energy source
6837415792malonate-assimilation
6837418394palatinose-builds acid from
6837415963ribitol-builds acid from
6837417306maltose+builds acid from
6837416899D-mannitol+builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837418024D-galacturonic acid+builds acid from
6837418403L-arabitol-builds acid from
6837416199urea-hydrolysis
6837425094lysine+degradation
6837429016arginine-hydrolysis
6837418257ornithine+degradation
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837435581indole+
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase+
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase+3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase+4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
358+-++----+--++-++++-+-
358+-++----+--++-++++-+-

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44629+-+--+--+-++--+-+-+--+--++----+-

Safety information

risk assessment

  • @ref: 358
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Escherichia coli NCTC10418GCA_900448155contigncbi562
66792Escherichia coli strain NCTC10418562.34140wgspatric562

External links

@ref: 358

culture collection no.: CCUG 3274, DSM 682, ATCC 10536, NCIB 8879, NCTC 10418

straininfo link

@refpassport
20218http://www.straininfo.net/strains/11400
20218http://www.straininfo.net/strains/134064
20218http://www.straininfo.net/strains/11403

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
358Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 682)https://www.dsmz.de/collection/catalogue/details/culture/DSM-682
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39734Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18778
44629Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 3274)https://www.ccug.se/strain?id=3274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E