Strain identifier

BacDive ID: 4420

Type strain: No

Species: Escherichia coli

Strain Designation: MacLeod

Strain history: CIP <- 1954, Centre de la Pénicilline, Paris, France: strain MacLeod

NCBI tax ID(s): 562 (species)

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General

@ref: 358

BacDive-ID: 4420

DSM-Number: 682

keywords: genome sequence, Bacteria, aerobe, mesophilic, motile, antibiotic resistance, human pathogen

description: Escherichia coli MacLeod is an aerobe, mesophilic, motile human pathogen that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

@refhistory
358<- NCTC <- E.J. Stokes
397341954, Centre de la Pénicilline, Paris, France: strain MacLeod
118689CIP <- 1954, Centre de la Pénicilline, Paris, France: strain MacLeod

doi: 10.13145/bacdive4420.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 358

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: MacLeod

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes94.552
6948099.996negative
118689yesnegativerod-shaped

colony morphology

  • @ref: 118689
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
358NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39734MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
118689CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
118689CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
358positivegrowth37mesophilic
39734positivegrowth30mesophilic
44629positivegrowth30-37mesophilic
118689positivegrowth30-41
118689nogrowth5psychrophilic
118689nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44629aerobe
118689facultative anaerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
358Mueller-Hinton Agar371aerob802432-342820-222838383428-3032-342424-2601003638142824016242026361036341000026
358Mueller-Hinton Agar371aerob10022322622283636322830242601003638142822-240142420-2224-2636-38028-3036800026-28
358Mueller-Hinton Agar37aerob10-12024342822283836-383830-32342828010-12036-3840142824-26016262428-303803636-388-1080028
358Mueller-Hinton Agar802430-3226222836-3836-38322830-32262801003636-3814-162824-26014-16362226-283803636800026-28
358Mueller-Hinton Agar80223228222838383426-2830-32222608-10034-363814282601424242438-4003436000028
358Mueller Hinton37aerob60223430263040403628-30342424012040421630-32260182420284003440000030

spore formation

@refspore formationconfidence
69481no100
69480no99.943

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
11868929864mannitol+fermentation
11868916947citrate-carbon source
1186894853esculin-hydrolysis
11868917234glucose+fermentation
11868917716lactose+fermentation
11868917632nitrate+reduction
11868916301nitrite+reduction
11868915792malonate-assimilation
118689132112sodium thiosulfate-builds gas from
11868917234glucose+degradation
6837418257ornithine+degradation
6837429016arginine-hydrolysis
6837425094lysine+degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid+builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol+builds acid from
6837417306maltose+builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan+energy source
6837417634D-glucose-builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose+builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose+builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

antibiotic resistance

  • @ref: 118689
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11868935581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837435581indole+
6836815688acetoin-
6836835581indole+
11868915688acetoin-
11868917234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase+
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase+3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase+4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
118689oxidase-
118689beta-galactosidase+3.2.1.23
118689alcohol dehydrogenase-1.1.1.1
118689gelatinase-
118689catalase+1.11.1.6
118689lysine decarboxylase+4.1.1.18
118689ornithine decarboxylase+4.1.1.17
118689tryptophan deaminase-
118689urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118689-++--++-+-++++++----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
358+-++----+--++-++++-+-
358+-++----+--++-++++-+-

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44629+-+--+--+-++--+-+-+--+--++----+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118689---------------------------------------------------------------------------------------------------

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
358yesyes2Risk group (German classification)
1186892Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Escherichia coli NCTC10418GCA_900448155contigncbi562
66792Escherichia coli strain NCTC10418562.34140wgspatric562

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.312no
anaerobicno93.666no
halophileno92.545no
spore-formingno94.054no
glucose-utilyes94.286no
thermophileno98.777no
aerobicyes83.195no
motileyes87.289no
flagellatedno62.91no
glucose-fermentyes90.976yes

External links

@ref: 358

culture collection no.: CCUG 3274, DSM 682, ATCC 10536, NCIB 8879, NCTC 10418, CIP 54.127, IMET 10907, NCIMB 8879

straininfo link

  • @ref: 73910
  • straininfo: 248455

Reference

@idauthorscataloguedoi/urltitle
358Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 682)https://www.dsmz.de/collection/catalogue/details/culture/DSM-682
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39734Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18778
44629Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 3274)https://www.ccug.se/strain?id=3274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
73910Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID248455.1StrainInfo: A central database for resolving microbial strain identifiers
118689Curators of the CIPCollection of Institut Pasteur (CIP 54.127)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2054.127