Strain identifier

BacDive ID: 4401

Type strain: Yes

Species: Winslowiella toletana

Strain Designation: A37

Strain history: CIP <- 2005, CECT <- 1999, G. de los Rios, San Pablo Univ., Madrid, Spain: strain A37

NCBI tax ID(s): 92490 (species)

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General

@ref: 7374

BacDive-ID: 4401

DSM-Number: 18073

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile

description: Winslowiella toletana A37 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from Olea europaea.

NCBI tax id

  • NCBI tax id: 92490
  • Matching level: species

strain history

@refhistory
7374<- CFBP <- J. E. G. de los Rios, A. M. Rojas; A37
123561CIP <- 2005, CECT <- 1999, G. de los Rios, San Pablo Univ., Madrid, Spain: strain A37

doi: 10.13145/bacdive4401.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Erwiniaceae
  • genus: Winslowiella
  • species: Winslowiella toletana
  • full scientific name: Winslowiella toletana (Rojas et al. 2004) Brady et al. 2023

  • synonyms

    • @ref: 20215
    • synonym: Erwinia toletana

@ref: 7374

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Erwiniaceae

genus: Erwinia

species: Erwinia toletana

full scientific name: Erwinia toletana Rojas et al. 2004

strain designation: A37

type strain: yes

Morphology

cell morphology

@refgram stainmotilitycell shape
30090negativeyes
123561negativeyesoval-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7374TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
40061Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
123561CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13
123561CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
7374positivegrowth30
30090positiveoptimum28
40061positivegrowth25
123561positivegrowth10-30
123561nogrowth5
123561nogrowth37
123561nogrowth41

Physiology and metabolism

oxygen tolerance

  • @ref: 123561
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3009030089acetate+carbon source
3009016449alanine+carbon source
3009022599arabinose+carbon source
3009017057cellobiose+carbon source
3009016947citrate+carbon source
3009017234glucose+carbon source
3009015792malonate+carbon source
3009029864mannitol+carbon source
3009028053melibiose+carbon source
30090506227N-acetylglucosamine+carbon source
3009026271proline+carbon source
3009017272propionate+carbon source
3009033942ribose+carbon source
3009017814salicin+carbon source
3009017822serine+carbon source
12356129864mannitol+fermentation
12356116947citrate+carbon source
12356117234glucose+fermentation
12356117716lactose-fermentation
12356117632nitrate+reduction
12356116301nitrite-reduction
12356115792malonate+assimilation
123561132112sodium thiosulfate-builds gas from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117113erythritol-builds acid from
68371Potassium 5-ketogluconate+builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from

antibiotic resistance

  • @ref: 123561
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123561
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12356115688acetoin+
12356117234glucose+

enzymes

@refvalueactivityec
30090catalase+1.11.1.6
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
123561oxidase-
123561beta-galactosidase+3.2.1.23
123561alcohol dehydrogenase-1.1.1.1
123561gelatinase-
123561catalase+1.11.1.6
123561lysine decarboxylase-4.1.1.18
123561ornithine decarboxylase-4.1.1.17
123561phenylalanine ammonia-lyase-4.3.1.24
123561tryptophan deaminase-
123561urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123561-+---+----++-+++-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123561+/--+/-+++--+/-++++-+-++---+++++++++-+------+-----+-++/-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123561+++++-+--+++++++++++++-+--+----+++--------+++----+++-+++++-++--------------+--+++-----+--+++-++----

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
7374Olea europaeaOlea europaeaToledo, Rana de la MascuraSpainESPEurope
123561Plant, Galls, Oliver TreeToledoSpainESPEurope

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Tree

taxonmaps

  • @ref: 69479
  • File name: preview.99_5840.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_502;98_4341;99_5840&stattab=map
  • Last taxonomy: Erwinia
  • 16S sequence: FR870447
  • Sequence Identity:
  • Total samples: 275
  • soil counts: 39
  • aquatic counts: 11
  • animal counts: 149
  • plant counts: 76

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
73741Risk group (German classification)
1235611Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Erwinia toletana partial 16S rRNA gene, type strain CECT 5263TFR8704471508nuccore92490
30090Erwinia toletana strain A4 16S ribosomal RNA gene, partial sequenceAF1308851493nuccore92490
7374Erwinia toletana strain A37 16S ribosomal RNA gene, partial sequenceAF1309101252nuccore92490

GC content

  • @ref: 30090
  • GC-content: 52

External links

@ref: 7374

culture collection no.: DSM 18073, ATCC 700880, CECT 5263, CFBP 6631, CIP 108675

straininfo link

  • @ref: 73892
  • straininfo: 91580

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15545461Erwinia toletana sp. nov., associated with Pseudomonas savastanoi-induced tree knots.Rojas AM, Rios JEGL, Saux MF, Jimenez P, Reche P, Bonneau S, Sutra L, Mathieu-Daude F, McClelland MInt J Syst Evol Microbiol10.1099/ijs.0.02924-02004Bacterial Typing Techniques, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Erwinia/*classification/cytology/*isolation & purification/metabolism, Genes, rRNA, Gentian Violet, Molecular Sequence Data, Nucleic Acid Hybridization, Olea/*microbiology, Phenazines, Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Pathogenicity36466697Transfer of Erwinia toletana and Erwinia iniecta to a novel genus Winslowiella gen. nov. as Winslowiella toletana comb. nov. and Winslowiella iniecta comb. nov. and description of Winslowiella arboricola sp. nov., isolated from bleeding cankers on broadleaf hosts.Brady C, Kaur S, Crampton B, Maddock D, Arnold D, Denman SFront Microbiol10.3389/fmicb.2022.10631072022Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7374Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18073)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18073
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
30090Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126447
40061Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6330
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73892Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID91580.1StrainInfo: A central database for resolving microbial strain identifiers
123561Curators of the CIPCollection of Institut Pasteur (CIP 108675)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108675