Strain identifier

BacDive ID: 44

Type strain: Yes

Species: Gluconacetobacter johannae

Strain Designation: CFN-Cf55, CFN-Cf55T

Strain history: CIP <- 2001, J. Caballero-Mellado, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM. Cuernavaca, Morelos, Mexico, strain: CFN-Cf55T <- T. Jimenez-Salgado

NCBI tax ID(s): 1307935 (strain), 112140 (species)

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General

@ref: 5103

BacDive-ID: 44

DSM-Number: 13595

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Gluconacetobacter johannae CFN-Cf55 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rhizosphere of coffee plants.

NCBI tax id

NCBI tax idMatching level
112140species
1307935strain

strain history

@refhistory
5103<- J. Caballero-Mellado <- T. Jiménez-Salgado
120354CIP <- 2001, J. Caballero-Mellado, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM. Cuernavaca, Morelos, Mexico, strain: CFN-Cf55T <- T. Jimenez-Salgado

doi: 10.13145/bacdive44.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Gluconacetobacter
  • species: Gluconacetobacter johannae
  • full scientific name: Gluconacetobacter johannae Fuentes-Ramírez et al. 2001

@ref: 5103

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Gluconacetobacter

species: Gluconacetobacter johannae

full scientific name: Gluconacetobacter johannae Fuentes-Ramírez et al. 2001

strain designation: CFN-Cf55, CFN-Cf55T

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
22919negative1.5-1.9 µm0.5-0.6 µmrod-shapedyesperitrichous
120354negativerod-shapedno

colony morphology

  • @ref: 22919
  • colony color: light-brown
  • medium used: potato agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5103GLUCONACETOBACTER JOHANNAE AND G. AZOTOCAPTANS MEDIUM (DSMZ Medium 920)yeshttps://mediadive.dsmz.de/medium/920Name: GLUCONACETOBACTER JOHANNAE AND G. AZOTOCAPTANS MEDIUM (DSMZ Medium 920) Composition: Agar 18.0 g/l K2HPO4 4.81 g/l MES buffer 4.4 g/l Glucose 2.7 g/l Yeast extract 2.7 g/l Mannitol 1.8 g/l KH2PO4 0.65 g/l Distilled water
22919LGIyes
22919potato agaryes
40689MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
120354CIP Medium 1yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1

culture temp

@refgrowthtypetemperaturerange
5103positivegrowth28mesophilic
22919positivegrowth29mesophilic
22919nogrowth37mesophilic
40689positivegrowth30mesophilic
120354positivegrowth22-30
120354nogrowth10psychrophilic
120354nogrowth37mesophilic
120354nogrowth41thermophilic
120354nogrowth45thermophilic

culture pH

  • @ref: 22919
  • ability: positive
  • type: growth
  • pH: 4-7
  • PH range: acidophile

Physiology and metabolism

tolerance

@refcompoundpercentage
22919Glucose30
22919Sucrose30

oxygen tolerance

@refoxygen tolerance
22919aerobe
120354obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
22919NaClpositivegrowth0.25-0.5 %
22919NaClnogrowth1 %
120354NaClpositivegrowth0 %
120354NaClnogrowth2 %
120354NaClnogrowth4 %
120354NaClnogrowth6 %
120354NaClnogrowth8 %
120354NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2291917108D-arabinose-growth
2291917754glycerol-growth
2291937684mannose-growth
2291917561L-cysteine-nitrogen source
2291929985L-glutamate-nitrogen source
2291917203L-proline-nitrogen source
2291917632nitrate-reduction
2291917634D-glucose+builds acid from
2291928120L-fructose+builds acid from
2291917992sucrose+builds acid from
2291928938ammonium+growth
2291912936D-galactose+growth
2291917634D-glucose+growth
2291916634raffinose+growth
2291917924D-sorbitol+growth
2291965327D-xylose+growth
2291916236ethanol+growth
2291917306maltose+growth
2291917992sucrose+growth
2291916828L-tryptophan+nitrogen source
2291930089acetate+oxidation
2291916236ethanol+oxidation
2291924996lactate+oxidation
2291928885butanol+/-growth
2291917108D-arabinose+/-growth
2291916634raffinose+/-growth
2291917924D-sorbitol+/-growth
2291917754glycerol+/-growth
2291917306maltose+/-growth
2291928053melibiose+/-growth
120354606565hippurate+hydrolysis
12035417632nitrate-reduction
12035416301nitrite-reduction

antibiotic resistance

  • @ref: 120354
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120354
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
22919catalase+1.11.1.6
22919cytochrome oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
120354oxidase-
120354beta-galactosidase+3.2.1.23
120354gelatinase-
120354catalase+1.11.1.6
120354gamma-glutamyltransferase-2.3.2.2
120354urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120354-----+--+-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120354+------+--++------------------------------------------------+-+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5103rhizosphere of coffee plantsChiapasMexicoMEXNorth America
120354Environment, Coffee plants, rhizosphereChiapasMexicoMEXNorth America1996

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_94670.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_64;97_2865;98_3566;99_94670&stattab=map
  • Last taxonomy: Gluconacetobacter
  • 16S sequence: JF793992
  • Sequence Identity:
  • Total samples: 159
  • soil counts: 25
  • aquatic counts: 18
  • animal counts: 66
  • plant counts: 50

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
51031Risk group (German classification)
1203541Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter sp. DOR 16S ribosomal RNA gene, partial sequenceAF1118411447ena112140
20218Gluconacetobacter johannae strain DSM 13595 16S ribosomal RNA gene, partial sequenceJF7939921358ena112140

GC content

  • @ref: 22919
  • GC-content: 57.96

External links

@ref: 5103

culture collection no.: DSM 13595, ATCC 700987, CIP 107160

straininfo link

  • @ref: 69729
  • straininfo: 86041

literature

  • topic: Phylogeny
  • Pubmed-ID: 11491326
  • title: Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants.
  • authors: Fuentes-Ramirez LE, Bustillos-Cristales R, Tapia-Hernandez A, Jimenez-Salgado T, Wang ET, Martinez-Romero E, Caballero-Mellado J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-4-1305
  • year: 2001
  • mesh: Acetic Acid/metabolism, Acetobacteraceae/*classification/genetics/*isolation & purification/metabolism, Base Sequence, Coffee/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Genes, Bacterial, Mexico, Molecular Sequence Data, Nitrogen Fixation, Nucleic Acid Hybridization, Phenotype, Phylogeny, Species Specificity, Terminology as Topic
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5103Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13595)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13595
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22919L E Fuentes-Ramírez, R Bustillos-Cristales, A Tapia-Hernández, T Jiménez-Salgado, E T Wang, E Martínez-Romero, J Caballero-Mellado10.1099/00207713-51-4-1305Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants.IJSEM 51: 1305-1314 200111491326
40689Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4565
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69729Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86041.1StrainInfo: A central database for resolving microbial strain identifiers
120354Curators of the CIPCollection of Institut Pasteur (CIP 107160)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107160